Author(s): V.L.N. Balaji Gupta Tiruveedhi, Venkateswara Rao Battula, Kishore Babu Bonige

Email(s): tvlbalaji79@gmail.com

DOI: 10.52711/0974-360X.2021.00947   

Address: V.L.N. Balaji Gupta Tiruveedhi1,2*, Venkateswara Rao Battula2, Kishore Babu Bonige2
1Department of Basic Science, Vishnu Institute of Technology (A), Bhimavaram, Andhra Pradesh, India.
2Department of Engineering Chemistry, Andhra University, Visakhapatnam, Andhra Pradesh, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
This research work was designed to establish and validate a novel stability indicating RP-HPLC method for the combined determination of Benidipine hydrochloride (BHE) and Nebivolol hydrochloride (NHE) in bulk and tablets, dependent on ICH guidelines.The assay method to analyse BHE and NHE was optimized with isocratic elution using acetonitrile: 0.1M acetate buffer (45:55, pH 5.1), Lichrospher ODS RP-18 column and flow pace of 1 ml/min. Total time for single run was 14 min. The injection quantity was 20µl, and was detected at 249nm. The method was verified on a concentration series of 1.25-10µg/ml (NHE) and 1.0-10µg/ml (BHE) for precision, accuracy and linearity. The LOD values were 0.059µg/ml and 0.028µg/ml for NHE and BHE, respectively. The LOQ values were 0.196µg/ml for NHE and 0.094µg/ml for BHE. The recovery percentages were 98.60-100.11% (BHE) and 98.94-101.50% (NHE) with relative standard deviation 0.250-0.694% (BHE) and 0.183-0.400% (NHE). The method was also observed to be efficient, and was sufficiently specific to measure BHE and NHE in the presence of stress-produced degradation products.


Cite this article:
V.L.N. Balaji Gupta Tiruveedhi, Venkateswara Rao Battula, Kishore Babu Bonige. Novel Determination of Anti-Hypertensive Combination; Benidipine Hydrochloride and Nebivolol Hydrochloride by High Performance Chromatographic Method. Research Journal of Pharmacy and Technology 2021; 14(10):5433-5438. doi: 10.52711/0974-360X.2021.00947

Cite(Electronic):
V.L.N. Balaji Gupta Tiruveedhi, Venkateswara Rao Battula, Kishore Babu Bonige. Novel Determination of Anti-Hypertensive Combination; Benidipine Hydrochloride and Nebivolol Hydrochloride by High Performance Chromatographic Method. Research Journal of Pharmacy and Technology 2021; 14(10):5433-5438. doi: 10.52711/0974-360X.2021.00947   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-65


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