Author(s): Ashpak. M. Tamboli, Kiran. A. Wadkar

Email(s): ashpak.tamboli@gmail.com

DOI: 10.52711/0974-360X.2021.00946   

Address: Ashpak. M. Tamboli1*, Kiran. A. Wadkar2
1Department of Pharmaceutical Chemistry, S.C.P.M, Sangola.
2Department of Pharmacognosy, A.B.C.P, Sangli.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
This study was subjected to investigate brine shrimp lethality bioassay of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) in different polarities of leaves extracts. Brine shrimp organisms were used to assess the cytotoxic activities of various polarities of leaves extracts while using brine shrimp lethality bioassay the cytotoxicity had been assessed and correlated with standard. Five concentrations (20, 50,100,200,500µg/ml) of each extract were used to find out the cytotoxicity. The Artemia salina lethality bioassay result was evaluated in terms of LC50 (lethality concentration).In each plant extracts into three replicates concentration naupli were added. After 24 h the surviving Artemia salina larvae were counted and LC50 was assessed. The cytotoxic activity of all polarities of extracts displayed activity within the value of LC50 (132.10–266.76 µg/ml) for Convolvulus pluricaulis and (213.86–279.47 µg/ml) for Mimosa rubicaulis (Lam.) Results revealed that the ethyl acetate extracts of Convolvulus pluricaulis and ethanolic extracts of Mimosa rubicaulis (Lam.) elicit significant activity compared to other extracts it indicated biologically active components are present in ethyl acetate and ethanolic extracts that could be accounted for its pharmacological effects.


Cite this article:
Ashpak. M. Tamboli, Kiran. A. Wadkar. Anticancer activity of leaves extracts of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) by Brine Shrimp Lethality Bioassay. Research Journal of Pharmacy and Technology 2021; 14(10):5427-2. doi: 10.52711/0974-360X.2021.00946

Cite(Electronic):
Ashpak. M. Tamboli, Kiran. A. Wadkar. Anticancer activity of leaves extracts of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) by Brine Shrimp Lethality Bioassay. Research Journal of Pharmacy and Technology 2021; 14(10):5427-2. doi: 10.52711/0974-360X.2021.00946   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-64


REFERENCES:
1.    Greenwell M, Rahman, PKSM, Medicinal plants: their use in anticancer treatment. Int. J. Pharm. Sci. Res.2015; (6): 4103–4112.
2.    Roy A., Shruti AS., Bharadvaja N. A review on medicinal plants against cancer. J. Plant Sci. Agric. Res. 2017; 2(1): 1–5.
3.    Willis J, A Dictionary of the Flowering Plant and Ferns. Cambridge University Press, USA,1996; 64–120.
4.    Ronakzahan M, AlamB, Saifal Islam, M, Gopal, C. Anti-cancer activity of Alangium salvifolium flower in enriches ascites carcinoma bearing mice. Int. J. Cancer Res. 2011; (7): 254–262.
5.    Preethi R, Devanathan VV, Loganathan M. Antimicrobial and antioxidant efficacy of some medicinal plants against food borne pathogens. Adv. Bio. Res. 2010; 4(2): 122–125.
6.    Maity P, Hansda D, Bandyopadhyay U, Mishra DK. Biological activities of crude extracts and chemical constituents of Bael, Aegle marmelos(L.) Corr. Indian J. Exp. Biol. 2009; (47): 849–861.
7.    Gibellini L, Pinti M, Nasi M, De Biasi, S., Roat E., Bertoncelli L., Cossarizza A. Interfering with ROS metabolism in cancer cells: the potential role of quercetin. Cancers 2010; (2): 1288–1311.
8.    Pritha Chakraborty, Shivani Sharma, Soumita Chakraborty, Ashwini Siddapurand, Jayanthi Abraham* Cytotoxicity and Antimicrobial Activity of Ipomoea batatas.Research J. Pharm. and Tech. July 2018; 11(7): 2741-46.
9.    Mavundza EJ, Tshikalange TE, Lall N., Hussein AA., Mudau, FN., Meyer, JJM. Antioxidant activity and cytotoxicity effect of flavonoids isolated from Athrixia phylicoides. J. Med. Plant Res.2010; (4): 2584–2587.
10.    Pezzuto JM., Plant-derived anticancer agents, Biochem. Pharmacol. 1997; 53 (2):121–133.
11.    SrivastavaV,Negi AS, KumarJK, Gupta M.M, Khanuja S.P, Plant-based  anticancer molecules: a chemical and biological profile of some important leads, Bioorg.   Med. Chem. 2005; 13(21): 5892–5908.
12.    Di Carlo G, Mascolo N, Izzo AA, Capassao F. Flavonoids: old and new aspects of a class of natural Therapeutic drugs. Life Sci 1999; 65: 337–353.
13.    .Keith MW, Sally AL, Michael WS,Thomas JG, Garry MM. Taxus Spp.Needles contain amounts of taxol comparable to the stem bark of Taxus brevifolia: analysis and isolation j Nat Prod 1990; 53: 1249–1255.
14.    Roja G, Heble MR. The quinoline alkaloid Camptothecin and 9-methoxy camptothecin from tissue cultures and mature trees of Nathapodytes foetida. Phytochem 1994; 36: 65–66.
15.    RamakrishnaYA, Manohor AI,Mamata P. Plants and novel anti-tumour agents; a review. Ind Drugs 1984; 21: 173–185.
16.     Indian pharmacopoeia. , the controller of publication; New Delhi, India 1996.
17.    Amarnath Reddy .G, Anil Chowdary. Y, Ravi.P, Madhavareddy.Pv,Anti-bacterial investigation on different root extracts of Mimosa rubicaulis(Lam.), Ijjpr;2010; 2(4): 18-21.
18.    Amir Muhammad, Rizwana Aleem Qureshi, Falzan Ullah, Zabtakhan Shinwari, Jafarkhan Flavanoids distribution in selected medicinal plants of Margalla hills and surroundings, Pak.j.Bot.,2010; 44(4);1241-1245.
19.    Singh RH, Mehta AK. Studies on the psychotropic effect of the Medhya Rasayana drug ‘Shankhpushpi’ (Convolvulus Pluricaulis) Part 1 (Clinical Studies). J Res Indian Med Yoga Homeopathy 1977; 12(3): 18.
20.    Sinha SN, Dixit VP, Madnawat AVS, Sharma OP. The possible potentiation of cognitive processing on administration of Convolvulus microphyllus in rats. Indian Med 1989; 1(3): 1-6.
21.    Shukla SP. Anti-anxiety agents of plant origin. Probe 1981; 20(3): 201.
22.    Singh GK, Bhandari A. Text book of Pharmacognosy, 1st ed. New Delhi: CBS Publishers: 2000; 193-94.
23.    Hossain MA., Zawan HM., Kawther, KR., Weli MA., Qasim, AR., Effect of temperature and extraction process on antioxidant activity of various leaves crude extracts of Thymus vulgaris. J. Coast Life Med. 2013; 1(2): 118–122.
24.    M. Kavya1, D. Priya2*Green Chemistry approach for the synthesis of some Benzimidazole Derivatives and their Biological Evaluation, Research J. Pharm. and Tech. March 2019; 12(3): 1023-1030.
25.    Afaf M. Weli, Ahmed Al-Harrasi, Noorulhuda H. Al Baiti, Anil Philip, Amzad Hossain, Syed Abdullah Gilani, Nadia Banioraba, Biological and toxicological evaluation of aerial parts extracts of locally grown Cleome austroarabica, Journal of King Saud University – Science. 2020; 32: 753–757.
26.    Mohammad Shah Hafez Kabir1, Mohammed Munawar Hossain1, Md. Imtiazul Kabir1, Md. Mominur Rahman1, Abul Hasanat1, Talha Bin Emran2, Md. Atiar Rahman, Phytochemical screening, Antioxidant, Thrombolytic, α-amylase inhibition and cytotoxic activities of ethanol extract of Steudnera colocasiifolia K. Koch leaves,  J Young Pharm, 2016; 8(4): 391-397.
27.    Meyer B, Ferrigni N, Putnam J et al. Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica 1982: 31-4.
28.    Sandeep B. Patil, Chandrakant S. Magdum. Determination of LC50 Values of Extracts of Euphorbia hirta Linn and Euphorbia nerifolia Linn Using Brine Shrimp Lethality Assay. Asian J. Res. Pharm. Sci. 1(3): July-Sept. 2011; Page 69-70.
29.    Rahman MA, Sultana R, Bin Emran T et al. Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity. BMC Complement Altern Med 2013; 13: 1472-6882.
30.    Saranya A, Nithya S* Assessment of Heavy Metal Induced Organ Toxicity in marketed Ayurvedhic Formulation and Report its LD50 value with Brine Shrimp Lethality Assay. Research J. Pharm. and Tech. January 2017; 10(1): 263-268.
31.    Md. Saif Uddin1, Mohammad Shah Hafez Kabir1, and Syed Md. Abdul Kader1* brine shrimp lethality bioassay, thrombolytic and antibacterial activities of methanol extract of Boehmeria platyphylla d don leaves Int J Pharm 2016; 6(1): 29-36.
32.    Krishnaraju, AV, Rao TVN, Sundararaju D, Vanisree M, Tsay HS, Subbaraju GV. Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. Int. J. Appl. Sci. Eng. 2005; 3: 125–134.
33.    McLaughlin J, Chang C, Smith D. Simple bench-top bioassays (brine shrimp and potato discs) for the discovery of plant anti-tumour compounds. In proceedings of the 18th National Seminar and UNESCO Workshop on Natural Products, Kuala Lumpur, Malaysia, and 20–25 September 1993.
34.    NasimunIslam N1., Jemimah Naine S.2, Shaik Jameel2, S.S. Khora2, Venkat Kumar, Mohanasrinivasan V.2, C. Subathra Devi2, Panneerselvam A.1*  Cytotoxic Property of Cocos nucifera shell Extracts on HeLa Cells Research J. Pharm. and Tech. May 2014;7(5): 521-525.
35.    Chowdhury TA, HasanatAbul *, Md.Jakaria, AT M .Mostofa Kamal, Thrombolytic and cytotoxic activity of methanolic extract of Commelina benghalensis (Family: Commelinaceae) Leaves Journal of Scientific and Innovative Research 2015; 4(2): 100-104.
36.    Nur Kuswanti1, 2, Sri Widyarti1, Widodo Widodo1, Muhaimin Rifa’i1*Cytotoxicity of Ethanolic Extract of Plumeria rubraL. Stem bark to Cancer Cells and Lymphocytes.Research J. Pharm. and Tech. December 2018; 11(12): 5545-5550.
37.    Ramasamy Arivukkarasu*, Aiyalu Rajasekaran, Vishal Kankaria, Madesh Selvam In Vitro Anti-Cancer Activity and detection of Quercetin, Apigenin in Methanol extract of Euphorbia nivulia Buch.-Ham. By HPTLC Technique. Research J. Pharm. and Tech. August 2017; 10(8): 2637-2640.
38.    B. Nagaraju1*, K. Sunitha1, A. Anitha2, and V.V. Rathnamma, Evaluation of the acute Toxicity of Profenofos and its Effect on the behavioural Changes in Freshwater Fish Labeo rohita Research J. Pharm. and Tech. February 2013; 6(2): 184-186.
39.    Mamatha A.*Brine Shrimp Lethality Test of Andrographis paniculataResearch J. Pharm. and Tech, July 2014; 7(7): 743-745.
40.    Jobin Jose*, R Narayana Charyulu, Prashant Nayak  In Vitro Cytotoxicity Studies of Pamam Dendrimer with an AntifungalAgent,Research J. Pharm. and Tech. January 2016; 9(1): 17-19.
41.    Swati Paul, Dibyajyoti Saha . Cytotoxic Activity of Ethanol Extract of Leea indica Leaf. Asian J. Res. Pharm. Sci. 2(4): Oct.-Dec. 2012; Page 137-139.

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