Girgis Samuel, Uddin Nazim, Ahmed S.G. Srag El-Din
Girgis Samuel1*, Uddin Nazim1, Ahmed S.G. Srag El-Din2
1Department of Pharmaceutical Science, School of Pharmacy, University of Sunderland.
2Department of Pharmaceutics, Faculty of Pharmacy, Delta University for Science and Technology, Egypt.
Volume - 14,
Issue - 10,
Year - 2021
The main objective of this project was to formulate novel amphiphilic PLGA nanoparticles having better physicochemical properties for the delivery of the novel peptide (CK-10) to be used for targeting the cancerous/tumour tissue. Double emulsion/Solvent evaporation and novel microfluidic techniques were used to formulate the nanoparticles. Loading efficiency and in-vitro release were measured by a modified Lowry assay. Size and zeta potential were characterized by dynamic light scattering, tuneable pore resistive sensing, and laser obscuration time. Images were scanned by scanning, transmission electron microscopes, and laser obscuration time. Stability was checked by high-performance liquid chromatography and capillary zone electrophoresis. Water absorption and its associated changes in the physicochemical properties were measured by various titration techniques. PLGA/Poloxomer nanoparticles had the highest peptide loading efficiency by 56.13 % for the novel microfluidic technique as well as the highest in-vitro release and water absorption values. It also had the smallest size with the lowest PDI (208.90 nm, 0.11) which are vital parameters for targeting cancer/tumour tissue. The successful development of better physicochemical properties for the CK-10 loaded PLGA nanoparticles could improve the RAN blocking by CK-10.
Cite this article:
Girgis Samuel, Uddin Nazim, Ahmed S.G. Srag El-Din. Optimization of PLGA nanoparticles for delivery of Novel anticancer CK-10 peptide. Research Journal of Pharmacy and Technology 2021; 14(10):5371-9. doi: 10.52711/0974-360X.2021.00937
Girgis Samuel, Uddin Nazim, Ahmed S.G. Srag El-Din. Optimization of PLGA nanoparticles for delivery of Novel anticancer CK-10 peptide. Research Journal of Pharmacy and Technology 2021; 14(10):5371-9. doi: 10.52711/0974-360X.2021.00937 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-55
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