Author(s): Girgis Samuel, Uddin Nazim, Ahmed S.G. Srag El-Din

Email(s): girgissamuel2@gmail.com

DOI: 10.52711/0974-360X.2021.00937   

Address: Girgis Samuel1*, Uddin Nazim1, Ahmed S.G. Srag El-Din2
1Department of Pharmaceutical Science, School of Pharmacy, University of Sunderland.
2Department of Pharmaceutics, Faculty of Pharmacy, Delta University for Science and Technology, Egypt.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
The main objective of this project was to formulate novel amphiphilic PLGA nanoparticles having better physicochemical properties for the delivery of the novel peptide (CK-10) to be used for targeting the cancerous/tumour tissue. Double emulsion/Solvent evaporation and novel microfluidic techniques were used to formulate the nanoparticles. Loading efficiency and in-vitro release were measured by a modified Lowry assay. Size and zeta potential were characterized by dynamic light scattering, tuneable pore resistive sensing, and laser obscuration time. Images were scanned by scanning, transmission electron microscopes, and laser obscuration time. Stability was checked by high-performance liquid chromatography and capillary zone electrophoresis. Water absorption and its associated changes in the physicochemical properties were measured by various titration techniques. PLGA/Poloxomer nanoparticles had the highest peptide loading efficiency by 56.13 % for the novel microfluidic technique as well as the highest in-vitro release and water absorption values. It also had the smallest size with the lowest PDI (208.90 nm, 0.11) which are vital parameters for targeting cancer/tumour tissue. The successful development of better physicochemical properties for the CK-10 loaded PLGA nanoparticles could improve the RAN blocking by CK-10.


Cite this article:
Girgis Samuel, Uddin Nazim, Ahmed S.G. Srag El-Din. Optimization of PLGA nanoparticles for delivery of Novel anticancer CK-10 peptide. Research Journal of Pharmacy and Technology 2021; 14(10):5371-9. doi: 10.52711/0974-360X.2021.00937

Cite(Electronic):
Girgis Samuel, Uddin Nazim, Ahmed S.G. Srag El-Din. Optimization of PLGA nanoparticles for delivery of Novel anticancer CK-10 peptide. Research Journal of Pharmacy and Technology 2021; 14(10):5371-9. doi: 10.52711/0974-360X.2021.00937   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-55


REFERENCES:
1.    Kohane DS. Microparticles and nanoparticles for drug delivery. Biotechnology and bioengineering. 2007; 96(2): 203-209.
2.    Sujani S, Babu RH, Reddy KR. Preparation of meloxicam spherical agglomerates to improve dissolution rate. Asian Journal of Pharmaceutical Research. 2012; 2(1): 32-36.
3.    Bankhele SC, Harale RB, Rao MR, Dhoka MV. Thermoreversible in-situ ophthalmic gelling system of levofloxacin hemihydrate: formulation and optimization by factorial design. Asian Journal of Pharmaceutical Research. 2012; 2(3): 100-106.
4.    Reddy YK, Tasleem J. Formulation and Evaluation of Bioadhesive Buccal Drug Delivery of Sumatriptan Succinate Tablets. Asian Journal of Pharmaceutical Research. 2020; 10(2): 105-109.
5.    Saiyyad IM, Bhambere D, Kshirsagar S. Formulation and Optimization of Silymarin Loaded PLGA Nanoparticle for liver targeting. Asian Journal of Pharmacy and Technology. 2017; 7(4): 209-220.
6.    Vasam E, Raj G. Photodegradation of Amido Black-10B on Nitrogen-doped Titania Nanoparticles under Visible Light Irradiation. Asian Journal of Research in Chemistry. 2012;5(7): 866-870.
7.    Acharya S, Sahoo SK. PLGA nanoparticles containing various anticancer agents and tumour delivery by EPR effect. Advanced drug delivery reviews. 2011; 63(3): 170-183.
8.    Wu XS. Preparation, characterization, and drug delivery applications of microspheres based on biodegradable lactic/glycolic acid polymers. Encyclopedic handbook of biomaterials and bioengineering. 1995: 1151-1200.
9.    Ahmed VA, Kumar H, Paranjothy K. Ophthalmic Drug Delivery of Diclofenac Potassium from Different Polymer Formulations: In Situ Sol Gels. Research Journal of Pharmaceutical Dosage Forms and Technology. 2009;1(2): 158-161.
10.    Saindane D, Kulkarni A, Sagri A, et al. Physicochemical Characterization of Solid Dispersion of Cefexime with Poloxamer 188. Research Journal of Pharmaceutical Dosage Forms and Technology. 2009; 1(2): 162-166.
11.    dos Santos PP, Flôres SH, de Oliveira Rios A, Chisté RC. Biodegradable polymers as wall materials to the synthesis of bioactive compound nanocapsules. Trends in Food Science and Technology. 2016; 3: 23-33.
12.    Patel V, Akbari B, Deshmukh A, Goyani M, Patel A. A Review on Long Acting PLGA Based in Situ Forming Microparticles Formulation for a Novel Drug Delivery System. Research Journal of Pharmaceutical Dosage Forms and Technology. 2016; 8(2): 127-134.
13.    Shaji J, Kumbhar M. Linezolid Loaded Biodegradable Polymeric Nanoparticles Formulation and Characterization. Research Journal of Pharmaceutical Dosage Forms and Technology. 2018; 10(4): 272-278.
14.    Rajarajan S, Chandramouli R. Preparation, Numerical Optimization and Evaluation of Ciprofloxacin PLGA and PLA Nanoparticles by Solvent Displacement Technique. Research Journal of Pharmacy and Technology. 2009;2(1):186-190.
15.    Bourdenx M, Daniel J, Genin E, et al. Nanoparticles restore lysosomal acidification defects: Implications for Parkinson and other lysosomal-related diseases. Autophagy. 2016;12(3):472-483.
16.    Bilati U, Allémann E, Doelker E. Poly (D, L-lactide-co-glycolide) protein-loaded nanoparticles prepared by the double emulsion method—processing and formulation issues for enhanced entrapment efficiency. Journal of microencapsulation. 2005;22(2):205-214.
17.    Jung T, Breitenbach A, Kissel T. Sulfobutylated poly (vinyl alcohol)-graft-poly (lactide-co-glycolide) s facilitate the preparation of small negatively charged biodegradable nanospheres. Journal of controlled release. 2000;67(2-3):157-169.
18.    Navarro SM, Swetledge S, Morgan T, et al. Biodistribution of orally administered poly (lactic-co-glycolic) acid nanoparticles for 7 days followed by 21 day recovery in F344 rats. NanoImpact. 2017;5:1-5.
19.    Owens III DE, Peppas NA. Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles. International journal of pharmaceutics. 2006;307(1):93-102.
20.    Buske J, König C, Bassarab S, Lamprecht A, Mühlau S, Wagner K. Influence of PEG in PEG–PLGA microspheres on particle properties and protein release. European journal of pharmaceutics and biopharmaceutics. 2012;81(1):57-63.
21.    Wei Y, Wang Y, Zhang H, Zhou W, Ma G. A novel strategy for the preparation of porous microspheres and its application in peptide drug loading. Journal of colloid and interface science. 2016;478:46-53.
22.    Kashi TSJ, Eskandarion S, Esfandyari-Manesh M, et al. Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method. International journal of nanomedicine. 2012;7:221.
23.    Lim E-K, Kim T, Paik S, Haam S, Huh Y-M, Lee K. Nanomaterials for theranostics: recent advances and future challenges. Chemical reviews. 2015;115(1):327-394.
24.    Kulhari H, Pooja D, Kota R, et al. Cyclic RGDfK peptide functionalized polymeric nanocarriers for targeting gemcitabine to ovarian cancer cells. Molecular pharmaceutics. 2016;13(5):1491-1500.
25.    Valencia PM, Farokhzad OC, Karnik R, Langer R. Microfluidic technologies for accelerating the clinical translation of nanoparticles. Nature nanotechnology. 2012;7(10):623-629.
26.    Bertrand N, Wu J, Xu X, Kamaly N, Farokhzad OC. Cancer nanotechnology: the impact of passive and active targeting in the era of modern cancer biology. Advanced drug delivery reviews. 2014;66:2-25.
27.    Xu L, Peng J, Yan M, Zhang D, Shen AQ. Droplet synthesis of silver nanoparticles by a microfluidic device. Chemical Engineering and Processing: Process Intensification. 2016;102:186-193.
28.    Khan IU, Serra CA, Anton N, Vandamme TF. Production of nanoparticle drug delivery systems with microfluidics tools. Expert opinion on drug delivery. 2015;12(4):547-562.
29.    Mirzaee I, Song M, Charmchi M, Sun H. A microfluidics-based on-chip impinger for airborne particle collection. Lab on a Chip. 2016;16(12):2254-2264.
30.    El-Menshawe SF, Ali AA, Halawa AA, El-Din ASS. A novel transdermal nanoethosomal gel of betahistine dihydrochloride for weight gain control: in-vitro and in-vivo characterization. Drug Design, Development and Therapy. 2017;11:3377-3388.
31.    Rahimian S, Fransen MF, Kleinovink JW, et al. Polymeric nanoparticles for co-delivery of synthetic long peptide antigen and poly IC as therapeutic cancer vaccine formulation. Journal of Controlled Release. 2015;203:16-22.
32.    Badri W, Miladi K, Nazari QA, Fessi H, Elaissari A. Effect of process and formulation parameters on polycaprolactone nanoparticles prepared by solvent displacement. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2017;516:238-244.
33.    D’Souza S, Dorati R, DeLuca PP. Effect of hydration on physicochemical properties of end-capped PLGA. Advances in Biomaterials. 2014;2014.
34.    Yáñez-Mó M, Siljander PR-M, Andreu Z, et al. Biological properties of extracellular vesicles and their physiological functions. Journal of extracellular vesicles. 2015;4(1):27066.

Recomonded Articles:

Author(s): R. Narayana Charyulu, P. Parvathy Devi, Jobin Jose, A. Veena Shetty

DOI: Not Available         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Pande S. D., Wagh A.S., Bhagure L.B., Patil S.G., Deshmukh A.R.

DOI: 10.5958/0974-360X.2015.00070.0         Access: Open Access Read More

Author(s): Vaseeha Banu T.S., Sandhya K.V., K.N. Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): Mouli Chandar. M Anton Smith. A

DOI: 10.5958/0974-360X.2019.00884.9         Access: Open Access Read More

Author(s): Sandesh More, Javed Mirza, Nanasaheb Kale, Mayur Gandhi, Rakesh Chaudhari

DOI: Not Available         Access: Open Access Read More

Author(s): Mayanka Singh, Manoj Charde, Rajesh Shukla, Rita M. Charde

DOI: Not Available         Access: Open Access Read More

Author(s): Moghal. Roohi Shabreen, S. Sangeetha

DOI: 10.5958/0974-360X.2020.00355.8         Access: Open Access Read More

Author(s): Poonam Karekar, Nitin Salunkhe, Adhikrao Yadav, Dnyaneshwar Bangar, Dhanashri Yadav

DOI: Not Available         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Samip Shah, T. Y. Pasha, Dipti Desai, Anil Bhandari

DOI: Not Available         Access: Open Access Read More

Author(s): Rituraj Singh Chundawat, Y.S. Sarangdevot, R.P.S. Rathore, Dharmendra Singh Sisodiya, Udaibhan Singh Rathore

DOI:         Access: Open Access Read More

Author(s): Anket Sharma, Vinod Kumar, Parminder Kaur, Ashwani Kumar Thukral, Renu Bhardwaj

DOI: 10.5958/0974-360X.2015.00299.1         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags