Author(s): Rini Hamsidi, Wahyuni, Adryan Fristiohady, Muhammad Hajrul Malaka, Idin Sahidin, Wiwied Ekasari, Aty Widyawaruyanti, Ahmad Fuad Hafid

Email(s): rini.hamsidi@vokasi.unair.ac.id

DOI: 10.52711/0974-360X.2021.00924   

Address: Rini Hamsidi1*, Wahyuni2, Adryan Fristiohady2, Muhammad Hajrul Malaka2, Idin Sahidin2, Wiwied Ekasari3, Aty Widyawaruyanti3,4, Ahmad Fuad Hafid3,4
1Department of Health, Faculty of Vocational Studies, Universitas Airlangga, Surabaya 60286, Indonesia.
2Faculty of Pharmacy, Universitas Halu Oleo, Kendari 93232, Indonesia.
3Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60115, Indonesia.
4Natural Product Medicine Research and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
Carthamus tinctorius Linn, also known as safflower, is a plant with the potential of being used in the production of antimalarial drugs. The purpose of this study was to isolate and identify the steroid compounds in the safflower and determine its antimalarial activity in vitro. The isolation process was conducted through extraction and chromatography methods. Then, the characterization of the isolated compounds was conducted through spectroscopic techniques which include Fourier Transform Infrared Spectroscopy (FT-IR), NMR 1-D (1H and 13C-NMR), and NMR 2-D (HMQC, HMBC, and H-H COZY) as well as comparing data with the existing literatures. In addition, the tests conducted were with variations of isolate concentrations (10, 1, 0.1, 0.01, and 0.001 µg/mL) against 3D7 strain of Plasmodium falciparum. Based on the FT-IR spectroscopic data, the steroid compounds isolated from safflowers might be stigmasterols. In addition, the isolates had -OH functional group in the region of 3431 cm-1, C-O in the region of 1053 cm-1, and Csp3-H in regions of 2960, 2934, and 2865 cm-1. The NMR 1-D data showed presence of 29 carbon atoms, while the protons were 48 in number. Furthermore, the IC50 value of the compound was 34.03 µg/mL with a percentage inhibition of 43.92% against the growth of P. falciparum. Therefore, it was classified as inactive agent in inhibiting the growth of malaria parasites, however, it could be used as a marker compound in C. tinctorius Linn extract.


Cite this article:
Rini Hamsidi, Wahyuni, Adryan Fristiohady, Muhammad Hajrul Malaka, Idin Sahidin, Wiwied Ekasari, Aty Widyawaruyanti, Ahmad Fuad Hafid. Steroid Compounds Isolation from Carthamus tinctorius Linn as Antimalarial. Research Journal of Pharmacy and Technology. 2021; 14(10):5297-4. doi: 10.52711/0974-360X.2021.00924

Cite(Electronic):
Rini Hamsidi, Wahyuni, Adryan Fristiohady, Muhammad Hajrul Malaka, Idin Sahidin, Wiwied Ekasari, Aty Widyawaruyanti, Ahmad Fuad Hafid. Steroid Compounds Isolation from Carthamus tinctorius Linn as Antimalarial. Research Journal of Pharmacy and Technology. 2021; 14(10):5297-4. doi: 10.52711/0974-360X.2021.00924   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-42


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