Ravindra Babu Sajja, Prasad Konduri, Eswar Kumar Kilari
Ravindra Babu Sajja1,3*, Prasad Konduri1, Eswar Kumar Kilari2
1Department of Pharmacology, Shri Vishnu College of Pharmacy, Bhimavaram, Andhrapradesh 534202.
2Department of Pharmacology, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530003.
3Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Secunderabad 500100.
Volume - 14,
Issue - 10,
Year - 2021
This work was mainly aimed to study the mast cell stabilizing, anti-anaphylactic and bronchodilatory activities of methanolic extract of Averrhoa carambola (ACME). Mast cell stabilization activity was investigated by Compound 48/80 induced mast cell degranulation in rats and antianaphylactic activity was performed by determining the mortality rate of mice upon exposure to compound 48/80. The bronchodilatory effect of ACME was studied on histamine aerosol-induced bronchospasm using guinea pigs, in which occurrence of preconvulsive dyspnea (PCD) was noted as end point. Treatment with ACME (100, 200 and 400mg/kg) showed significant (p<0.05) protection of rat peritoneal mast cells and significantly (p<0.05) reduced the mortality of mice in a dose dependent manner. ACME significantly (p<0.05) increased the time of preconvulsive dyspnea (PCD) in a dose dependent manner that suggestive of bronchodilating activity. Phytochemical studies observed presence of saponins, tannins, steroids, alkaloids, flavonoids and glycosides. From these finding, we concluded that ACME possesses mast cell stabilizing; anti anaphylactic and bronchodilatory activity which might be used in treatment of asthma.
Cite this article:
Ravindra Babu Sajja, Prasad Konduri, Eswar Kumar Kilari. Mast Cell Stabilizing, Antianaphylactic and Bronchodilatory activity of Methanolic extract of Averrhoa carambola Fruit. Research Journal of Pharmacy and Technology. 2021; 14(10):5258-3. doi: 10.52711/0974-360X.2021.00916
Ravindra Babu Sajja, Prasad Konduri, Eswar Kumar Kilari. Mast Cell Stabilizing, Antianaphylactic and Bronchodilatory activity of Methanolic extract of Averrhoa carambola Fruit. Research Journal of Pharmacy and Technology. 2021; 14(10):5258-3. doi: 10.52711/0974-360X.2021.00916 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-34
1. Suresh R, Mhaske GP, Nehru Sai Suresh CH, Ashok Kumar J, Benito Johnson D, Venkatanarayanan R. Pharmacological Evaluation of Antiasthmatic Activity of Tamarindus indica Seed. Research J. Pharmacology and Pharmacodynamics. 2011; 3(3): 115-122.
2. Susanta R, Saptarshi D, Bhabagrahi R. Evaluation of Broncho-Protective Effect of Zingiber officinale Roscoe in Histamine Induced Broncho-Spasm. Research J. Pharm. and Tech. 2010; 3(2): 589-591.
3. Da Silva EZ, Jamur MC, Oliver C. Mast cell function: a new vision of an old cell. J Histochem Cytochem. 2014; 62(10): 698–738.
4. Pavithra H. Dave, Preetha. Pathogenesis and Novel Drug for Treatment of Asthma – A Review. Research J. Pharm. and Tech 2016; 9(9):1519-1523. doi: 10.5958/0974-360X.2016.00297.3
5. Church MK, Levi-Schaffer F. The human mast cell. J. Allergy Clin. Immunol. 1997 Feb; 99(2): 155-160. doi: 10.1016/s0091-6749(97)70089-7.
6. Petersen LJ, Mosbech H, Skov PS. Allergen-induced histamine release in intact human skin in vivo assessed by skin microdialysis technique: characterization of factors influencing histamine releasability. J. Allergy Clin Immunol. 1996; 97(2): 672- 679.
7. Jain VK, Dheeraj A, Jain B, Ahirwar B. Evaluation of Acute Oral Toxicity and Mast Cell Degranulation of an aqueous ethanolic extract of Tritium aestivum Linn. Research J. Pharm. and Tech 2018; 11(2):643-648. doi: 10.5958/0974-360X.2018.00120.8
8. Ennis M, Pearce FL, Weston PM. Some studies on the release of histamine from mast cells stimulated with polylysine. British Journal of Pharmacology. 1980 Oct; 70(2):329–334. doi: 10.1111/j.1476-5381. 1980.tb07940.x
9. Kim MS, Na HJ, Han SW, Jin JS, Song UY, Lee EJ, Song BK, Hong SH, Kim HM. Forsythiafructus inhibits the mast-cell mediated allergic inﬂammatory reactions. Inﬂammation. 2003 Jun; 27:129–135. doi: 10.1023/a: 1023865727780.
10. Madhavachetty K, Sivaji K, Tulasi Rao K. Flowering Plants of chittoor District. 1st Ed. Tirupati: Students off set printers; 2008.p.55.
11. Warrier PK, Nambiar VPK, Ramankutty C. Indian medicinal plants: A compendium of 500 species. Vol. III. Chennai: Orient Longman Ltd; 1994.p. 423.
12. Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Dehradun: International Book Distributors; 1987. p. 355-440.
13. 13.Peach D, Tracey MV. Modem Methods of Plant Analysis. Berlin: Springer-Verlog; 1955. p.373-374
14. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 30th ed. Pune: Nirali Prakashan; 1996. p. 447.
15. Evans WC. Trease and Evans’ Pharmaconosy. 15th ed. London: WB Sounders Company Ltd; 2005.p. 224, 230, 336, 541.
16. Patel LS, Patel RS. Preliminary Phytochemical Analysis and Antimicrobial Activity of In -vitro Condition Asparagus racemosus Willd. leaf. Research J. Pharm. and Tech. 2013; 6(12):1387-1390.
17. Balamurugan G, Arunkumar MP, Muthusamy P, Anbazhagan S. Preliminary Phytochemical Screening, Free radical Scavenging and Antimicrobial activities of Justicia tranquebariensis Linn. Research J. Pharm. and Tech. 2008; 1(2):116-118.
18. 18.Vijayalakshmi A, Suganya M, Pankaj Kumar Giri, Jayakumari S, Shanmugasundaram P. Bronchodilator and Mast Cell Stabilizer Effect of Siddha Formulation Seenthil Chooranam. Research J. Pharm. and Tech. 2017; 10(1): 252-256. doi: 10.5958/0974-360X.2017. 00052. X
19. Gupta PP, Srimal RC, Srivastava M, Singh KL and Tandon JS. Antiallergic activity of Arbortristosides from Nyctanthus arbortristis. International Journal of Pharmacognosy. 1995; 33(1): 70–72.
20. Bharti A, Dheeraj A, Ram A. Antihistaminic Effect of Sitopaladi Churna Extract. Research J. Pharm. and Tech. 2008; 1(2): 89-92.
21. Suralkar Anupama A, Jadhav Asha S, Vaidya Gayatri S, Ashish S, Meghraj Chaudary, Vivek B. Antiallergic, Antianaphylactic and Mast Cell Stabilizing, Activity of Embelia ribes brum. Research J. Pharm. and Tech. 2013; 6(11): 1190-1194.
22. Chitme HR, Mallikarjun M, Chandrashekhar VM, Prasant PM. Antiallergic activity of Aristolochia bracteolata Lank in animal model. Indian J Exp Biol. 2010Jan; 48(1): 46–52.
23. Sheth UK, Dadkar NK, Kamat UG. Selected topics in experimental pharmacology. 1st ed. Bombay: Kothari book depot; 1972. p.5, 63.
24. Patel T, Rajshekar C, Parmar R. Mast cell stabilizing activity of Myrica nagi bark. J Pharmacog Phytother. 2011; 3(8): 114-117.
25. Suh WM, Park SB, Lee S, Kim HH, Suk K, Son JH, et al. Suppression of mast-cell-mediated allergic inflammation by Lindera obtusiloba. Exp Biol Med. 2011; 236 (2): 240-246. DOI: 10.1258/ebm.2010.010274
26. Kim SH, Jun CD, Suk K, Choi BJ, Seunja P, Lee SH, et al. Gallic acid inhibits histamine release and pro-inflammatory cytokine production in mast cells. Toxicol Sci. 2006 May; 91(1): 123-131. doi: 10.1093/toxsci/kfj063. Epub 2005 Dec 1.
27. John RK, Zutshi U, Kameshwaran L. Effect of quercitin and Albizzia saponins on rat mast cell. Indian Journal of Physiology and Pharmacology. 1985; 29(1): 43–46.
28. Gupta SS, Tripathi RM. Effect of chronic treatment of the saponin of Clerodendron serratum on disruption of the mesenteric mast cells of rats. Aspects of Allergy and Applied Immunology. 1973; 4: 177–188.
29. Mahajan SG, Mehta AA.Inhibitory Action of Ethanolic Extract of Seeds of Moringa oleifera Lam. On Systemic and Local Anaphylaxis. Journal of Immunotoxicology.2007; 4(4):287 – 294.https://doi.org/10.1080/15476910701680137