Author(s): Nastiti Faradilla Ramadhani, Alexander P. Nugraha, Igo S. Ihsan, Yoni A. Agung, Fedik A. Rantam, Diah S. Ernawati, Rini D. Ridwan, Ida B. Narmada, Arif N. M. Ansori, Suhaila Hayaza, Tengku N.E.B.T.A. Noor

Email(s): alexander.patera.nugraha@fkg.unair.ac.id

DOI: 10.52711/0974-360X.2021.00911   

Address: Nastiti Faradilla Ramadhani1,2, Alexander P. Nugraha1,2,3*, Igo S. Ihsan4, Yoni A. Agung5, Fedik A. Rantam6, Diah S. Ernawati7, Rini D. Ridwan8, Ida B. Narmada2,3, Arif N. M. Ansori9, Suhaila Hayaza10, Tengku N.E.B.T.A. Noor11
1Graduate Student of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Dental and Biomaterial Research Group, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
3Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
4Stem Cell Research and Development Center, Universitas Airlangga Surabaya, Surabaya, Indonesia.
5Dental Medicine Research Center, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
6Laboratory of Virology, Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
7Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indones

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
The gingival medicinal signaling cells conditioned medium (GMSCs-CM) is a biocompatible material which possessed beneficial cytokine, anti-microbial peptide, growth factor that can be collected after culture. GMSCs- CM may inhibit bone resorption in order to improve the patient’s quality of life. In this study, the potential effect of GMSCs-CM on the number of osteoclasts and osteoblasts in Lipopolysaccharide (LPS)-induced calvaria bone resorption in wistar rats (Rattus novergicus) has been analyzed. Twenty-eight male and healthy wistar rats (R. novergicus) at the age of 1-2 months old with 250-300 grams body weight were divided into 4 groups, namely PBS group: 100µg PBS day 1-7; LPS group: 100µg LPS day 1-7; LPS and GMSCs group: 100µg LPS + 100µg GMSCS-CM day 1 1-7, GMSCs group: 100µg M-GMSCs day 1-7. Escherichia Coli LPS was used to induce the bone resorption on the calvaria with subcutaneous injection. GMSCs-CM was collected after passage 4-5 then injected subcutaneously on the calvaria. All samples were examined on the. 8th day through cervical dislocation. The number of osteoblasts and osteoclasts in calvaria was then observed under 400x magnification. One Way ANOVA and Tukey HSD were conducted to analyze differences between groups (p<0.01). The number of osteoclasts in calvaria decreased significantly in the LPS + GMSCs-CM group compared to LPS group (p<0.01). The number of osteoblasts in calvaria increased significantly in the LPS + GMSCs-CM group compared to LPS group (p<0.01). GMSCs-CM can reduce the amount of osteoclast significantly and increases the production of osteoblast in LPS-induced calvaria bone resorption in wistar rats (R. novergicus).


Cite this article:
Nastiti Faradilla Ramadhani, Alexander P. Nugraha, Igo S. Ihsan, Yoni A. Agung, Fedik A. Rantam, Diah S. Ernawati, Rini D. Ridwan, Ida B. Narmada, Arif N. M. Ansori, Suhaila Hayaza, Tengku N.E.B.T.A. Noor. Gingival Medicinal Signaling Cells Conditioned Medium effect on the Osteoclast and Osteoblast number in Lipopolysaccharide-induced Calvaria Bone Resorption in Wistar Rats’ (Rattus novergicus). Research Journal of Pharmacy and Technology. 2021; 14(10):5232-7. doi: 10.52711/0974-360X.2021.00911

Cite(Electronic):
Nastiti Faradilla Ramadhani, Alexander P. Nugraha, Igo S. Ihsan, Yoni A. Agung, Fedik A. Rantam, Diah S. Ernawati, Rini D. Ridwan, Ida B. Narmada, Arif N. M. Ansori, Suhaila Hayaza, Tengku N.E.B.T.A. Noor. Gingival Medicinal Signaling Cells Conditioned Medium effect on the Osteoclast and Osteoblast number in Lipopolysaccharide-induced Calvaria Bone Resorption in Wistar Rats’ (Rattus novergicus). Research Journal of Pharmacy and Technology. 2021; 14(10):5232-7. doi: 10.52711/0974-360X.2021.00911   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-29


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