Author(s): Krishnaveni R, S. Rajan


DOI: 10.52711/0974-360X.2021.00908   

Address: Krishnaveni* R, Dr. S. Rajan
PG and Research Department of Microbiology, M.R. Govt. Arts College, Mannargudi, Affiliated to Bharathidasan University, Thiruchirapalli, Tamil Nadu - India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021

Clerodendrum phlomid is was a common shrub of arid plains, low hills, and tropical deserts. It belongs to the family Verbenaceae. Many species of this genus have been documented in traditional system of medicine in India. The roots of the plant have been extensively studied in Ayurveda but in folk and traditional medicine, it is the leaf that finds more application in alleviating many diseases. Oxidation byproducts of metabolism was responsible for many pathophysiological conditions. Anti-oxidants are molecules that quench the free radicals. The present study aims at evaluating the in-vitro anti-oxidant and anti-inflammatory activity of the aqueous and ethanol leaf extract of Clerodendrum phlomidis. The anti-oxidant activity was evaluated by three in vitro models, namely the DPPH radical scavenging activity, hydroxyl radical scavenging activity and NO radical scavenging activity. The anti-inflammatory activity was evaluated by the RBC membrane Inhibition method. The aqueous extract was found to have better anti- oxidant activity than the ethanol extract in DPPH and NO radical scavenging activity. The RBC membrane inhibition method clearly states that the aqueous leaf extract of Clerodendrum phlomidis was found to have a comparatively an effective anti-inflammatory activity than ethanol extract.

Cite this article:
Krishnaveni R, S. Rajan. In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F. Research Journal of Pharmacy and Technology. 2021; 14(10):5217-1. doi: 10.52711/0974-360X.2021.00908

Krishnaveni R, S. Rajan. In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F. Research Journal of Pharmacy and Technology. 2021; 14(10):5217-1. doi: 10.52711/0974-360X.2021.00908   Available on:

1.    Gowri R and Madhavan V. Evaluation of Antioxidant activity of Ethanolic extract of Sphaeranthus amaranthoides Burm. f. International Journal of Drug delivery and Research. 2013; 5(4): 320-329.
2.    Sharmistha Banerjee, Shuchi Kaushik, Rajesh Singh Tomar. Efficacy Assay of Crude Leaf Extracts of Calotropis procera and Azadirachta indica for Anti-oxidant Activity. Research J. Pharm. and Tech 2018; 11(10): 4480-4486. doi: 10.5958/0974- 360X.2018.00820.X
3.    Bruce N Ames, Mark K. Shigenaga, and Tory M. Hagen. Oxidants, antioxidants, and the degenerative diseases of aging Proc. Natl. Acad. Sci. USA. 1993:90:7915-7922.
4.    Rekha Rajendran, Hemachander R, Ezhilarasan T, Keerthana C, Saroja DL, Saichand KV, Mohamed Gasim Abdullah. Phytochemical Analysis and In-Vitro Antioxidant Activity of Mimosa pudica Lin., Leaves. Research J. Pharm. and Tech. 2010; 3(2): 551-555.
5.    B. Suneetha, K.V.S.R.G. Prasad, B.R. Soumya, P. Deepthi Nishantha, B. Sampath Kumar, Rajaneekar D. Evaluation of In- vitro anti-oxidant activity of various extracts of Actinodaphne madraspatana leaves. Res. J. Pharmacognosy and Phytochem. 2014; 6(1): 01-04.
6.    M. Elayarani, P. Shanmuganathan, P. Muthukumaran. In Vitro Anti-Oxidant Activity of    the Various Extracts of Cassia auriculata L. Flower by UV Spectrophotometer. Asian J. Pharm. Tech. 1(3): July-Sept. 2011; Page 70-72.
7.    Rohit Kumar Bargah, Anil Kushwaha, Arun Tirkey, Bimlesh Hariwanshi. In Vitro Antioxidant and Antibacterial Screening of flowers Extract from Cassia auriculata Linn. Research J. Pharm. and Tech 2020; 13(6): 2624-2628.
8.    Deepa babu, Prema Gurumurthy, Sai Krishna Bora and KM Cherian. Anti-oxidant and free radical scavenging activity of triphala determined by using different invitro models. Journal of Medicinal plant research.2013; 7(39): 2898-2905.
9.    Jabeen A, Rani S and Ibrahim M. HPTLC finger printing and anti- oxidant activity of Lepidium sativum and Tigonella foenum seeds. International Journal of Biology, Pharmacy and Allied sciences.2020;9(7): 1582-1593.
10.    Vani Mamillapalli, Ratna Harika Chapala, Tejaswi Komal Sai Sareddu, Latha Sri Kondaveeti, Santhi Pattipati, Padmalatha Khantamneni. Evaluation of Phytochemical and in Vitro Anti- Inflammatory activity of Leaf and Fruit Extracts of Casuarina equisetifolia. Asian J. Pharm. Tech. 2020; 10(3):143-148. doi: 10.5958/2231-5713.2020.00025.2
11.    Moharram HA and MM Youseff. Methods for determining the Anti-oxidant activity: A review. Alex. J. Fd. Sci and technol.2014; 11(1): 31-42
12.    Uma Sankar Gorla, M. Savithri, G.S.N. Koteswara Rao, Y. Niharika, P. Devi Sree Sathya, V. Harika. Evaluation of anti- inflammatory activity of Hydroalcoholic extract of Ananas cosmosus fruit peel by HRBC membrane stabilization. Asian J. Pharm. Res. 2018; 8(1):33-35. doi: 10.5958/2231- 5691.2018.00006.0
13.    Swati Jain, Nilesh Gupta, UK Jain, AS Raghuvanshi, Ajay Patel. Anti-Inflammatory Activity of Butea monosperma Leaves. Research J. Pharmacology and Pharmacodynamics. 2010; 2(5): 351-352.
14.    Anusha. D, Nagaraju Kancherla, Darling Chellathai. In vitro Screening of Antioxidant, Anti-Inflammatory activity of Vitex negundo Methanolic leaf extract. Research J. Pharm. and Tech. 2019; 12(6): 2824-2827. doi: 10.5958/0974-360X.2019. 00475.X
15.    .Dewasya Pratap Singh, Brijeshkunvar Mishra , Richa Mishra. Anti-nociceptive and Anti-inflammatory Activity of Annona squamosa L. Leaf Extract in Mice and Rats. Research J. Pharmacognosy and Phytochemistry 2012; 4(3): 182-185.
16.    Salha Mahdjar, Chahrazad Bakka, Hocine Dendougui, Mohamed Hadjadj. Phytochemical profile and In vitro Anti-inflammatory Activity of Anvillea radiata (Coss and Dur) flowers Extracts; Asian J. Research Chem. 2020; 13(1): 44-47. doi: 10.5958/0974- 4150.2020.00010.3
17.    Evalyn Asante Kwantia, Abraham Yebsah Mensah and Michael Frimpong Baidoo. Analgesic and anti-inflammatory effect of Ghanian medicinal plants-2020.DOI: w.5772/intechopen.90154.
18.    Jin Win Wang, Fei Luan, Xiang Dong He, Yong Wong and Mao Xinghi . Traditional uses and Pharmacological properties of Clerodendrum Phytochemicals. Journal of traditional and complementary medicine. 2018;(8):24-38
19.    Muthu kumaradoss Mohan Maruga Raja and Sri Hari Mishra. Comprehensive review of Clerodendrum phlomidis: a traditionally used bitter. Journal of Chinese integrative medicine. 2010; 8(6): 510-525
20.    Kokate CK. Practical Pharmacognosy. New Delhi, Vallabh Prakashan, 1999, pp 107-121.
21.    Harborne JB. Phytochemical Methods. 3rd ed. London, Chapman and Hall, 1998, pp 4-7.
22.    Blois MS. Antioxidant determination by the use of stable free radical. Nature 1958; 181: 1199 –1200.
23.    Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethylsulphoxide by hydroxyl radical generating system. Biochemistry. 1991;20: 6006–6012.
24.    Thabrew MI, Robin D. Hughes ,Ian G. McFarlane. Antioxidant activity of Osbeckia aspera. Phyto Therapy Research.1998;12 (4):288-290.
25.    Green LC, Wagner DA, J. Glogowski J, Skipper PL Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and(15N) nitrate in biological fluids. Anal. Biochem. 1982; 126(1):131-138.
26.    Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti- inflammatory activity of methonal extract of Oxalis corniculate linn. International Journal of Pharma and Pharmacological Sciences (2010); 2(1): 146-155.
27.    Mishra A., Sharma A.K., Kumar S., Saxena A.K., Pandey
A.K. Bauhinia variegata leaf extracts exhibit considerable antibacterial, antioxidant, and anticancer activities. Biomed. Res. Int. 2013;2013: 915436. doi: 10.1155/2013/915436.
28.        Meng X.H., Liu C., Fan R., Zhu L.F., Yang S.X., Zhu H.T., Wang D., Yang C.R., Zhang Y.J. Antioxidative flavan-3-ol dimers from the   leaves   of Camellia   fangchengensis. J.   Agric.    Food Chem. 2018; 66:247–254. doi: 10.1021/acs.jafc.7b04572
29.    Middleton E., Kandaswami C. Effects of flavonoids on immune and inflammatory cell functions. Biochem. Pharmacol. 1992; 43:1167–1179. doi: 10.1016/0006-2952(92)90489-6.
30.        Vasantha Rupasinghe H.P., Boehm M.M.A., Sekhon-Loodu S., Parmar I., Bors B., Jamieson A.R. Anti-inflammatory activity of haskap cultivars is polyphenols-dependent. Biomolecules. 2015;5: 1079–1098. doi: 10.3390/biom5021079
31.    Kumar Singh S., Patra A. Evaluation of phenolic composition, antioxidant, anti-inflammatory and anticancer activities of Polygonatum verticillatum (L.) J. Integr. Med. 2018;16: 273– 282. doi: 10.1016/j.joim.2018.04.005

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available