Amr A. El-Waseif, Hussein S. Gaber, Emad A. Ewais
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Amr A. El-Waseif*, Hussein S. Gaber, Emad A. Ewais
Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
Volume - 14,
Issue - 10,
Year - 2021
The Hypocholesterolemic is an effective and friendly process for the removal of cholesterol and an important aspect of human health. Removal of cholesterol by probiotic bacteria was investigated in this study. The potential probiotic bacterium was preliminarily identified as Lactobacillus casei. In addition, the various operating parameters like cholesterol concentration, incubation time, bile salts concentration, probiotic dose and initial pH was studied. The optimum parameters for most probiotics studied are cholesterol concentration is 100 µg /ml, incubation time is 48 h, bile salts concentration is 0.5%, probiotic dose is 200µl and initial pH is 6. The maximum percentage removal of cholesterol using Lactobacillus casei is 93.6. The probiotics bacteria are favorable for Hypocholesterolemic.
Cite this article:
Amr A. El-Waseif, Hussein S. Gaber, Emad A. Ewais. Hypocholesterolemic Operating Parameters of Novel Probiotics In vitro. Research Journal of Pharmacy and Technology. 2021; 14(10):5197-1. doi: 10.52711/0974-360X.2021.00904
Amr A. El-Waseif, Hussein S. Gaber, Emad A. Ewais. Hypocholesterolemic Operating Parameters of Novel Probiotics In vitro. Research Journal of Pharmacy and Technology. 2021; 14(10):5197-1. doi: 10.52711/0974-360X.2021.00904 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-22
1. FAO/WHO, 2001. Report of a Joint FAO/WHO expert consultation on evaluation of health nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. World Health Organization and Food and Agriculture Organization of the United Nations, London, Ontario, Canada.
2. Indira M., Venkateswarulu T.C., Abraham Peele K., Vidya Prabhakar K., Krupanidhi S. Characterization of Bacteriocin Producing Probiotic Properties of Enterococcus casseliflavus MI001 Isolated from Curd Sample. Current Trends in Biotechnology and Pharmacy. 2019; 13(1): 64-71.
3. Ezhumalai M., G. Hemalatha, J.P. Poornima, K.V. Pugalendi. Inhibition of Lactobacillus growth by amino acids and phytochemicals in the fermentation of curd by disc diffusion method. Asian J. Pharm. Res. 2013; 3(4): 189-193.
4. Shanthi V., Jemima Florence Borgia, Bhavani S. and Sathya M. Inhibitory Effects of Lactobacillus Species against Human Pathogens. Research J. Pharmacognosy and Phytochemistry. 2011; 3(4): 174-177.
5. Achanta H. and Suneetha V. Isolation and Biochemical Characterization of Probiotic Lactobacillus Species Isolated from Curd Samples of Southern Regions of Vellore, Tamil Nadu. Research J. Pharm. and Tech. 2017; 10(6): 1734-1741.
6. Hossain M. K., Nahar K., Shokryazdan P., Abdullah N., Hamid K., and Jahromi M. F. Probiotic Potential of Lactic Acid Bacteria Isolated from Cheese, Yogurt and Poultry Faeces. Research J. Pharm. and Tech. 2017; 10(9): 2991-2998.
7. Nguyen H. H., Nguyen D. A. and Nguyen T. K. Biological Activities of poly (Lactic acid) polymer produced from Lactobacillus rhamnosus PN04. Research J. Pharm. and Tech. 2018; 11(7): 3057-3062.
8. Raju M. K., Kapali B. S., Jason Jeba Singh G. V., Y. Subathra and Dernita Maria Nithya A. Isolation, Characterization and Sequencing of Lactobacillus from the Oral and Fecal Samples of Healthy Dogs. Research J. Pharm. and Tech. 2018; 11(11): 5061- 5065.
9. Laili R. D., Martati E. and Rifa’i M. Immunomodulator effect of Moringa oleifera Leaves Fermented by Lactobacillus plantarum FNCC 0137 on Salmonella typhi infected Balb/C Mice. Research J. Pharm. and Tech. 2019; 12(8): 3595-3601.
10. Chauhan S. B., Singh V. and Chauhan R. Enteric coated Microbeads as a Potential Delivery System for improved probiotic effect of Lactobacillus rhamnosus GG. Research J. Pharm. and Tech. 2019; 12(12): 6049-6056.
11. Haroun B. M., Refaat B. M., El-Waseif A. A., El- Menoufy H. A. and Amin H. A. Hypolipidemic Activity of the Probiotic Lactobacillus plantarum NRRL B-4496 and their Prebiotic Exopolysaccharide In Vitro and In Vivo, Journal of Applied Sciences Research. 2013; 9(1): 1015-1020.
12. De Jongh, S., Ose L., Szamosi T., Gagne C., Lambert M., Scott R., Perron P., Dobbelaere D., Saborio M., Tuohy M.B., Stepanavage M., Sapre A., Gumbiner B., Mercuri M., Van Trotsenburg A.S., Bakker H.D., and Kastelein J.J. Efficacy and Safety of Statin Therapy in Children with Familial Hypercholesterolemia: A Randomized, Double-Blind, Placebo-Controlled Trial with Simvastatin. Circulation. 2002; 106: 2231-7.
13. Shehata M. G., El-Sahn M. A., El Sohaimy S. A. and Youssef M.
M. In vitro assessment of hypocholesterolemic activity of Lactococcus lactis subsp. Lactis, Bulletin of the National Research Centre. 2019; 43: 60-69.
14. Fazeli, H., Moshtaghian J., Mirlohi M., and Shirzadi M. Reduction in serum lipid parameters by incorporation of a native strain of Lactobacillus plantarum A7 in Mice. Iranian Journal of Diabetes and Lipid Disorders. 2010; 9: 1-7.
15. Bangaraiah P. Optimization of Operating Parameters Using Response Surface Method for Biosorption of Zinc. Current Trends in Biotechnology and Pharmacy. 2020; 14(1): 7-15.
16. De Man, J.C., Rogosa M., and Sharpe E. A medium for the cultivation of Lactobacilli. J. Appl. Bacteriol. 1960; 23: 130-135.
17. Shehata M. G, El Sohaimy S. A, El-Sahn M. A. and Youssef M.
M. Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity. Ann Agri Sci. 2016; 61: 65–75.
18. Ong, Y.Y., Wen S. T., Rosfarizan, M., Sieo, C.C. and Beng, T. T. Biochemical and molecular identification of Enterococcus spp. from red pitaya. Process Biochemistry. 2014; 49: 563-568.
19. Artiss JD. and Zak B. Measurement of cholesterol concentration. In: Rifai N, Warnick GR, Dominiczak MH, eds. Handbook of lipoprotein testing. Washington: AACC Press; 1997: 99-114.
20. Lye, H., Rahmat-Ali G. R., and Liong M. Mechanisms of cholesterol removal by lactobacilli under conditions that mimic the human gastrointestinal tract. International Dairy Journal. 2010; 20: 169-175.
21. Kimoto H., Ohmomo S., and Okamoto T. Cholesterol Removal from Media by Lactococci. American Dairy Science Association 2002; 85: 3182-3188.
22. Liong M.T. and Shah N.P. Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of lactobacilli strains. Int. Dairy J. 2005; 15: 391-398.
23. Kim G.B, Yi S.H and Lee B.H. Purification and characterization of three different types of bile salt hydrolases from Bifidobacterium strains. J Dairy Sci. 2004; 87: 258-66.
24. Jaleel S. and Kiliç A. O. Antimicrobial Action of isolated Probiotic Lactobacillus plantarum from Different Fermented Dairy Products from Trabzon City. Research J. Pharm. and Tech. 2020; 13(5): 2445-2451.
25. Sundaramoorthy S., Selvaraj S. and Kameshwari V. Antibacterial Activity of Lactobacillus Species Isolated from Dairy products against Antibiotics. Research J. Pharm. and Tech. 2020; 13(8): 3915-3917.