Author(s): Fatimah A. Jasim, Hameed Salman Al-Hilu

Email(s): ,

DOI: 10.52711/0974-360X.2021.00897   

Address: Fatimah A. Jasim1, Hameed Salman Al-Hilu2 Histopathology, Biology Instructor, The Gifted Students' School- Basra, Ministry of Education Basra, Iraq, 61030.
2Fourth Stage, The Gifted Students’ School- Basra, Iraq, 61030.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021

Garlic is one of many medicinal plants that used over thousands of years due to its organosulfur compounds. Materials and methods: commercial garlic was used for the extraction, phytochemicals detections and Gas Chromatography-Mass (GC- Mass) analysis utilized to determine the main compounds in the aqueous extract. Antioxidant activity was evaluated in the garlic extract using total antioxidant capacity (TAC) and DPPH for detecting the concentration that takes half of the free radicals. The phytochemical tests revealed several compounds in the extract, included alkaloid, glycosides, tannins, and flavonoid and phenol while saponin compound was not detected. GC-Mass spotted 15 different compounds included allyl trisulfide with the concentration (1.89%) followed by ascorbic acid 2, 6-dihexadecanoate (22.24%), 9, 12-Octadecadienoic acid (Z, Z) (11.44), and 6-Octadecenoic acid, (Z) (40, 28). Total Antioxidant Activity of garlic aqueous extract reached 60 mg/ml compared to that of ascorbic acid. IC50 examination observed that the aqueous extract of garlic had 308.57µg/ml value.

Cite this article:
Fatimah A. Jasim, Hameed Salman Al-Hilu. Chemical Datasets, Antioxidant, Free Radicals Scavenger activities estimate in Aqueous Garlic (Allium sativum) extract. Research Journal of Pharmacy and Technology. 2021; 14(10):5157-2. doi: 10.52711/0974-360X.2021.00897

Fatimah A. Jasim, Hameed Salman Al-Hilu. Chemical Datasets, Antioxidant, Free Radicals Scavenger activities estimate in Aqueous Garlic (Allium sativum) extract. Research Journal of Pharmacy and Technology. 2021; 14(10):5157-2. doi: 10.52711/0974-360X.2021.00897   Available on:

1.    Rangasamy P, Hansiya VS, Maheswari PU, Suman T, Geetha N. Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoria ternatea L. Blue Flowered Leaves. Asian J Pharm Anal. 2019; 9(2): 67. Available from:
2.    Gini EJ, Sivakkumar T, Kuppuswami S. Determination of antioxidant activity of various extracts of pajanelia longifolia (Willd.) K. Schum, isolation and characterization of flavonoid from ethanol extract by column chromatography. Res J Pharm Technol. 2017 Oct 1; 10(10): 3391–7.
3.    Saleh NE, Michael FR, Toutou MM. Evaluation of garlic and onion powder as phyto-additives in the diet of sea bass (Dicentrarcus labrax). Egypt J Aquat Res. 2015; 41(2): 211–7. Available from:
4.    El-Sebaey AM, Abdelhamid FM, Abdalla OA. Protective effects of garlic extract against hematological alterations, immunosuppression, hepatic oxidative stress, and renal damage induced by cyclophosphamide in rats. Environ Sci Pollut Res. 2019;
5.    Stearn WT. HOW MANY SPECIES OF ALLIUM ARE KNOWN?. The Kew Magazine. Wiley; 1992, (9) :180–2. Available from:
6.    Arfa ABEN, Najjaa H, Yahia B, Tlig A, Neffati M. Antioxidant capacity and phenolic composition as a function of genetic diversity of wild Tunisian leek (Allium ampeloprasum L.). 2015; 21(2): 957–68.
7.    Labrador JRP, Guinares RC, Hontiveros GJS. Effect of garlic powder- supplemented diets on the growth and survival of Pacific white leg shrimp (Litopenaeus vannamei). Cogent Food Agric. 2016; 2(1). Available from:
8.    Warshafsky S. Effect of Garlic on Total Serum Cholesterol. Ann Intern Med. 1993; 119(7_Part_1): 599. Available from:
9.    Sharifi-Rad M, Mnayer D, Tabanelli G, Stojanovic-Radic ZZ, Sharifi- Rad M, Yousaf Z, et al. Plants of the genus Allium as antibacterial agents: From tradition to pharmacy. Cell Mol Biol. 2016; 62(9):57–68.
10.    Pyun MS, Shin S. Antifungal effects of the volatile oils from Allium plants against Trichophyton species and synergism of the oils with ketoconazole. Phytomedicine. 2006; 13(6): 394–400.
11.    Alyasari HF, Al-Khafaji JKT, Al-Masoudi HK. Inhibitory effects of garlic extract on uropathogenic escherichia coli; proteus mirabilis and trichomonas vaginalis isolated from urogenital tract cases. Res J Pharm Technol. 2018; 11(3): 1071–7.
12.    Shang A, Cao SY, Xu XY, Gan RY, Tang GY, Corke H, et al. Bioactive compounds and biological functions of garlic (allium sativum L.). Foods. 2019; 8(7): 1–31.
13.    Bayan L, Koulivand PH, Gorji A. Garlic: a review of potential therapeutic effects. Avicenna J phytomedicine. 2014; 4(1):1–14. Available from:
%0A 103721
14.    Alam K, Abu M, Nyeem B, Samad A. Effects of garlic on hyperlipidemia : A review. 2018; 6(2): 44–8.
15.    Parvathi P. Garlic-A golden wonder. Res J Pharm Technol.; 2018 (11): 393–6.    Available    from:
16.    Sandeep DS, Nayak P, Jose J, Rishal Relita M, Sumana DR. Formulation and evaluation of antibacterial herbal gels of Murraya koenigii leaves extract. Res J Pharm Technol. 2017; 10(6):1798–801.
17.    Thomson M, Alnaqeeb MA, Bordia T, Al-Hassan JM, Afzal M, Ali M. Effects of aqueous extract of onion on the liver and lung of rats. J Ethnopharmacol. 1998; 61(2): 91–9.
18.    Harborne JB. Phytochemical Methods. second edi. Chapman and Hall. London New York: Chapman and Hall; 1984.
19.    Parthiban C, Saranya C. Evaluation of in vitro antioxidant properties of some selected seaweeds from Tuticorin coast. Int J Curr Microbiol … 2013;    2(9):64–73.    Available    from:
_vitro_antioxidant_properties_of_some_selected_seaweeds_from_Tuti corin_coast/file/3deec5230546e1f3f6.pdf
20.    Godavari A, Amutha K. In vitro Antidiabetic Activity of Garcinia mangostana by Enzymatic Inhibition Assay. Res J Pharm Technol. 2017; 10(2): 508.
21.    Anusha D, Kancherla N, Chellathai D. In vitro Screening of Antioxidant, Anti-Inflammatory activity of Vitex negundo Methanolic leaf extract. Res J Pharm Technol. 2019; 12(6): 2824.
22.    Rahman A, Rahman MM, Mominul M, Sheik I, Rahman MM, Shadli SM, et al. Free radical scavenging activity and phenolic content of Cassia sophera L. African J Biotechnol. 2008; 7(10):
1591–3. Available from:
23.    Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci Technol. 1995; 28(1): 25–30.
24.    Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011; 48(4): 412–22.
25.    Shang HM, Zhou HZ, Yang JY, Li R, Song H, Wu HX. In vitro and in vivo antioxidant activities of inulin. PLoS One. 2018; 13(2): 1–12.
26.    Gorinstein S, Leontowicz H, Leontowicz M, Drzewiecki J, Najman K, Katrich E, et al. Raw and boiled garlic enhances plasma antioxidant activity and improves plasma lipid metabolism in cholesterol-fed rats. Life Sci. 2006; 78(6): 655–63.
27.    Jastrzebski Z, Leontowicz H, Leontowicz M, Namiesnik J, Zachwieja Z, Barton H, et al. The bioactivity of processed garlic (Allium sativum L.) as shown in vitro and in vivo studies on rats. Food Chem Toxicol. 2007; 45(9): 1626–33.
28.    Rahman MM, Fazlic V, Saad NM. Antioxidant properties of raw garlic (Allium sativum) extract. Int Food Res J. 2012; 19(2): 589–91.
29.    Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004; 74(17): 2157–84.
30.    El-Demerdash FM, Yousef MI, El-Naga NIA. Biochemical study on the hypoglycemic effects of onion and garlic in alloxan-induced diabetic rats. Food Chem Toxicol. 2005; 43(1): 57–63.
31.    Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. Food Chem. 2001; 49: 5165–70.
32.    Sun J, Chu Y-F, Wu X, Liu RH. Antioxidant and antiproliferative activities of common fruits. JAgric Food Chem. 2002; 50: 7449–54.
33.    Al Asaad N, Al Diab D. Determination of total antioxidant activity of fruit juices widely consumed in Syria. Res J Pharm Technol. 2017; 10(4):957.
34.    Teeli RA, Ganie SA, Dar MS, Raja W, Yadav SS. Antioxidant activity of Euphorbia hirta l. Leaf extracts. Res J Pharm Technol. 2018 Jan 1; 11(1):199–202.    Available    from:
35.    Pal R, Girhepunge K, Shrivastava N, Hussain MM, N T. Antioxidant and Free Radical Scavenging Activity of Ethanolic Extract of Morinda citrifolia. Res J Pharm Technol. 2011; 4(8): 1224–6. Available from: Journal of Pharmacy and Technology; PID=2011-4-8-4
36.    Ataei ML, Ebrahimzadeh-Bideskan AR. The effects of nano-silver and garlic administration during pregnancy on neuron apoptosis in rat offspring hippocampus. Iran J Basic Med Sci. 2014; 17(6): 411–8.
37.    Bozin B, Mimica-Dukic N, Samojlik I, Goran A, Igic R. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem. 2008; 111(4): 925–9.
38.    Lanzotti V. The analysis of onion and garlic. J Chromatogr A. 2006; 1112(1–2): 3–22.
39.    Shetty S, Udupa S, Udupa L. Evaluation of antioxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats. Evidence-based Complement Altern Med. 2008; 5(1): 95– 101.
40.    Gorinstein S, Park YS, Heo BG, Namiesnik J, Leontowicz H, Leontowicz M, et al. A comparative study of phenolic compounds and antioxidant and antiproliferative activities in frequently consumed raw vegetables. Eur Food Res Technol. 2009; 228(6): 903–11.
41.    Zouari Chekki R, Snoussi A, Hamrouni I, Bouzouita N. Chemical composition, antibacterial and antioxidant activities of Tunisian garlic (Allium sativum) essential oil and ethanol extract. Mediterr J Chem. 2014; 3(4): 947–56.
42.    Lawrence R, Lawrence K. Antioxidant activity of garlic essential oil (Allium Sativum) grown in north Indian plains. Asian Pac J Trop Biomed. 2011 Sep 1; 1(SUPPL. 1): S51–4.
43.    Hemalatha A, Girija K, Parthiban C, Saranya C, Anantharaman P. Antioxidant properties and total phenolic content of a marine diatom, Navicula clavata and green microalgae, Chlorella marina and Dunaliella salina. Pelagia Res Libr. 2013; 4(5): 151–7.

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