Author(s): Erna Prawita Setyowati, Purwantiningsih, Fidya Maulina Yulianny Erawan, Suci Rahmanti, Ni’mah Rifka Hanum, Natasya Cendikia Moeksa Devi

Email(s): erna_prawita @ ugm.ac.id

DOI: 10.52711/0974-360X.2021.00893   

Address: Erna Prawita Setyowati*1,2, Purwantiningsih1,3, Fidya Maulina Yulianny Erawan1, Suci Rahmanti1, Ni’mah Rifka Hanum1, Natasya Cendikia Moeksa Devi1
1Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.
2Department of Pharmaceutical Biology, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.
3Department of Pharmacology, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.
*Corresponding Author

Published In:   Volume - 14,      Issue - 10,     Year - 2021


ABSTRACT:
This study was to investigate the cytotoxic and antimicrobial activities of ethyl acetate extract yielded from fermented fungi Trichoderma reesei strain: JCM 2267, Aspergillus flavus strain MC-10-L, Penicillium sp, and Aspergillus fumigatus associated with Stylissa flabelliformis sponge, and also to investigate the significant component in ethyl acetate extract of it. Antimicrobial experimentation was performed to various microbes with liquid microdilution method. The assessment of antimicrobials was undertaken by observing the value of MIC50. Cytotoxic testing was performed using MTT assay method. The cytotoxic activity evaluation was conducted by finding the value of IC50. The compound analysis in the ethyl acetate extract was used GC-MS. The result showed that in the antimicrobial experimentation, there was a variation in the percentage of inhibition where the smallest MIC50 values were found in Aspergillus fumigatus fungi ethyl acetate extract against Candida albicans ATCC 10231 at 1.3?g/mL. The result of the cytotoxic test against tumor cell line T47D, the lowest IC50 was found in Penicillium sp fungi with 111?g/mL value. Aspergillus flavus strain MC-10-L fungi, Penicillium sp, and Aspergillus fumigatus obtained from sponge isolation Stylissa flabelliformis have antimicrobial and cytotoxic activity with various range. All fungi extracts are not toxic against normal cells (Vero cells). The class of compounds in ethyl acetate fungi extract Trichoderma reesei strain JCM 2267 are mostly cyclohexane.


Cite this article:
Erna Prawita Setyowati, Purwantiningsih, Fidya Maulina Yulianny Erawan, Suci Rahmanti, Ni’mah Rifka Hanum, Natasya Cendikia Moeksa Devi. Cytotoxic and Antimicrobial Activities of Ethyl Acetate Extract from Fungus Trichoderma reesei strain JCM 2267, Aspergillus flavus strain MC- 10-L, Penicillium sp, and Aspergillus fumigatus Associated with Marine Sponge Stylissa flabelliformis. Research Journal of Pharmacy and Technology. 2021; 14(10):5126-2. doi: 10.52711/0974-360X.2021.00893

Cite(Electronic):
Erna Prawita Setyowati, Purwantiningsih, Fidya Maulina Yulianny Erawan, Suci Rahmanti, Ni’mah Rifka Hanum, Natasya Cendikia Moeksa Devi. Cytotoxic and Antimicrobial Activities of Ethyl Acetate Extract from Fungus Trichoderma reesei strain JCM 2267, Aspergillus flavus strain MC- 10-L, Penicillium sp, and Aspergillus fumigatus Associated with Marine Sponge Stylissa flabelliformis. Research Journal of Pharmacy and Technology. 2021; 14(10):5126-2. doi: 10.52711/0974-360X.2021.00893   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-10-11


REFERENCES:
1.    Ashwini K, Kumar S. Partial-Purification of Alpha-Amylase from Marine Streptomyces gancidicus-ASD_KT852565. Research J. Pharm and Tech. 2016. 9(6); 731-734
2.    Badisa RB, Reed SFD, Joseph P, Cooper hood JS, Latinwo LM, Goodman CB. Selective Cytotoxic Activities of Two Novel Synthetic Drugs on Human Breast Carcinoma MCF-7 Cells. Anticancer Res, 2009; 29 (8), 2993-2996.
3.    Balouiri M, Sadiki M, Ibnsouda K. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 2015; 6 (2), 71-79.
4.    Brickmann M, Marker A, Kurtböke DI. An Overview on Marine Sponge-symbiotic bacteria as Unex hausted source natural product Discovery Diversity, 2017; 9 (40), 1-31.
5.    CCRC (Cancer Chemoprevention Research Center). Protocol of Cytotoxic assay (MTT Methods). 2013. Faculty of Pharmacy, Gadjah Mada University, Yogyakarta.
6.    Chhibber S, Bansal S, Kaur S. Disrupting the mixed-species biofilm of Klebsiella pneumoniae B5055 and Pseudomonas aeruginosa PAO using bacteriophages alone or in combination with xylitol. Microbiology, 2015; 161, 1369-1377.
7.    CLSI (Clinical and Laboratory Standards Institute). Methods for Dilution Antimicrobial Susceptibility Test for Bacteria that Grow Aerobically; Approved Standard, 7th Edition, 2006. Wayne, Pensylvania.
8.    Cohen E., Koch L., Thu K.M., Rahamim Y., Aluma Y., Ilan M., Yarden O., Carmeli S. Novel terpenoids of the fungus Aspergillus insuetus isolated from the Mediterranean sponge Psammocinia sp. collected along the coast of Israel. Bioorgan. Med. Chem. 2011; 19, 6587–6593.
9.    Devi, NCM, Aktivitas Sitotoksik Ekstrak Etil Asetat Fungi Sal 3 Asosiasi Spons Stylissa flabelliformis Terhadap Sel Kanker T47d Dengan Metode Mtt Assay, 2018, Undergraduate Thesis, Universitas Gadjah Mada, Yogyakarta, Indonesia
10.    Devi SS, Sreenivasulu Y, Rao KVB. Talaromyces verruculosus, A Novel Marine Fungi as a Potent Polyhydroxybutyrate Degrader. Research J. Pharm and Tech. 2014. 7(4); 433-438
11.    Dobretsov S, Dahms HU, Qian PY. Inhibition of Biofouling by Marine Microorganisms and Their Metabolites. Biofouling, 2006; 22, 43–54.
12.    Dogan SM, Ercetin AP, Altun Z, Dursun D, Aktas S. Gene expression characteristic of breast cancer stem cell. J BUON, 2015; 20, 1304-1313.
13.    Egan S, Thomas T, Kjelleberg S. Unlocking the Diversity and Biotechnological Potential of Marine Surface Associated Microbial Communities. Current Opinion in Microbiology, 2008; 11, 219–25.
14.    Erawan, FMY, Uji Aktivitas Sitotoksik Ekstrak Etil Asetat Metabolit Fungi Kode Sal 9 Asosiasi Spons Stylissa Flabelliformis Terhadap Sel T47d Secara In Vitro, 2018, Undergraduate Thesis, Universitas Gadjah Mada, Yogyakarta, Indonesia
15.    Gopi M, Kumaran S, Kumar TTA, Deivasigamani B, Alagappan K, Prasad SG. Antibacterial Potential of Sponge Endosymbiont Marine Enterobacter sp. at Kavaratti Island, Lakshadweep Archipelago. Asian Pacific Journal of Tropical Medicine, 2012; 5, 142–46.
16.    Hanum, NR, Potensi Ekstrak Etil Asetat Fungi Kode Sal 6 Asosiasi Spons Stylissa Flabelliformis Dalam Menghasilkan Senyawa Sitotoksik Terhadap Sel Kanker Payudara T47d (Metode MTT Assay), 2018, Undergraduate Thesis, Universitas Gadjah Mada, Yogyakarta, Indonesia
17.    Hentschel U, Usher KM, Taylor T. Marine Sponges as Microbial Fermenters. FEMS Microbiology Ecology, 2006; 55, 167–77.
18.    Holliday DL and Speirs V. Choosing Right Cell Line for Breast Cancer Research. Breast Cancer Research, 2011; 13, 215.
19.    Indraningrat AA, Smidt H, Sipkema D. Bioprospecting Sponge- Associated Microbes for Antimicrobial Compounds. Mar. Drug, 2016; 14 (5).
20.    Jadulco R, Proksch, P., Wray, V., Sudarsono, Berg, A., and Graffe, U., 2001, New macrolides and furan carboxylic acid derivative from the sponge-derived fungus Cladosporium herbarum. J Nat Prod, 64 (4), 527-530.
21.    Jayaprakashyel GK, Abundance M. Diversity and Prospect of Marine fungi and Biotechnological Application. Research J. Pharm and Tech. 2017. 10(12); 4386-4388.
22.    Larichev VF, Smirnova IP, Syatkin SP, Myandina GI, Chibisov SM, Ryskina EA. Effect of L-lysine-α-Oxidase from Trichoderma In Vitro Experiment on The model of Viruses as Sindbis, Tick- borne encephalistis, West Nile, Tahyna and Dhori. Research J. Pharm and Tech. 2017. 10(3); 765-768
23.    Kolarevic S, Milovanovic, D., Avdovic, M., 2016, Optimisation оf the microdilution method for detection of minimum inhibitory concentration values in selected bacteria. Botanica Serbica, 40 (1), 29-36.
24.    König GM, Kehraus S, Seibert SF, Lateff SA, Müller D. Natural Products from Marine Organisms and Their Associated Microbes. Chembiochem, 2006; 7, 229–38.
25.    Lee YM, Li H, Hong J, Cho HY, Bae KS, Kim MA, Kim DK, Jung JH. Bioactive metabolites from the sponge-derived fungus Aspergillus versicolor. Arch. Pharm. Res. 2010; 33: 231–235.
26.    Liu Y, Marmann A, Abdel-Aziz MS, Wang CY, Müller WEG, Lin H., Mándi A, Kurtán T, Daletos G, Proksch P.tetrahydroanthraquinonee Derivatives from the Endophytic Fungus Stemphylium globuliferum. Eur. J. Org. Chem. 2015; 2646–2653.
27.    Maboni G, Gressler LT, Espindola JP, Schwab M, Tasca C, Potter L, Vargas AC. Differences in the antimicrobial susceptibility profiles of Moraxella bovis, M. bovocul, M .ovis. Brazilian Journal of Microbiology, 2015; 46 (2), 545-549.
28.    Martins A, Viera H, Gaspar H, Santos S. Marketed Marine Natural Products in the Pharmaceutical and Cosmeceutical Industries: Tips for Success. Mar Drugs, 2014; 12 (2), 1066-1101.
29.    Mehbub MF, Lei JC, Zhang W. Marine Sponge Derived Natural Products between 2001 and 2010: Trends and Opportunities for Discovery of Bioactive. Mar. Drugs, 2-14; 12(8), 4539-4577.
30.    Muralihdaran A, Chandrasekhar R, Rao JV. Marine Microbial Metabolites as Drug Candidate for Alzheimer’s Disease. Research J. Pharm and Tech. 2019. 12(12); 6081-6086
31.    Muralihdaran V, Deecaraman M. A Source of Novel Therapeutic Drugs-Marine Actinomycetes. Research J. Pharm and Tech. 2017. 10(10); 3598-3600
32.    Nunes BC, Martins MM, Chang R, Morais SAL, Nascimento EA, Oliveira A, Cunha LCS, Silva CV, Teixeira TL, Ambrosio MALV, Martins CHG, Aquino FJT. Antimicrobial activity, cytotoxicity and selectivity index of Banisteriopsis laevifolia (A. Juss.) B. Gates leaves. Industrial Corp and product, 2016; 92, 277-289.
33.    Ozaki T, Nakagawara A. Role of P53 in Cell Death and Human Cancers. Cancers, 2011; 3, 994-1013.
34.    Proksch P, Edrada RA, Ebel, R. Drugs from the Seas-Current Status and Microbiological Implications. Applied Microbiology Biotechnology, 2002; 59, 125–34.
35.    Quave CL, Plano LRW, Pantuso T, Bennet BC. Effect of extract from Italian Medicinal Plant on Planktonic Growth, BiofilmFormationo, and Adherence of Methicillin Resistantt Staphylococcus aureus. Journal Ethnopharmacoll, 2008; 118 (3), 418-428.
36.    Pitamber J, Hitender P, Pradeep S, Kumar SRS, Veena S, Rao KVB. Production of L-Asparaginase by Marine Actinobacteria Isolated from the Rhizosphere of Mangrove Plants. Research J. Pharm and Tech. 2014. 5(6); 526-530
37.    Prayong P, Barusrux S, and Weerapreeyakul N. Cytotoxic Activity Screening of Some Indigenous Thai Plants. Fitoterapia, 2008; 79, 598-601.
38.    Rahmanti, S, Aktivitas Sitotoksik Ekstrak Etil Asetat Metabolit Fungi Kode Sal 7 Asosiasi Spons Stylissa flabelliformis Terhadap Sel T47d Dengan Metode MTT Assay, 2018, Undergraduate Thesis, Universitas Gadjah Mada, Yogyakarta, Indonesia
39.    Rio JL, Recio MC. Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology, 2005; 100: 80-84.
40.    Rungprom W, Siwu ERO, Lambert LK. Cyclic Tetrapeptides from Marine Bacteria Associated with the Seaweed Diginea sp. and the Sponge Halisarca ectofibrosa. Tetrahedron, 2008; 64, 3147–3152.
41.    Sarkar A, Philip AM, Thakker DP, Wagh MS, Rao KVB. In vitro Antioxidant activity of extracellular L-glutaminose enzyme isolated from marine yeast Rhodopola sp. DAMB. Research J. Pharm and Tech. 2020. 13(1); 209-215
 
42.    Schafer JM, Lee ES, O’regam RM, Yao K, Jordan VC. Rapid Development of Tamoxifen-Stimulated Mutant P53 Breast Tumors (T47D) In Athymic Mice. Clin. Cancer Res, 2000; 6, 4373-4380.
43.    Selvin J, Shanmughapriya S, Gandhimathi R, Seghal KS, Rajeetha RR, Natarajaseenivasan K, Hema TA. Optimization and production of novel antimicrobial agents from sponge associated marine actinomycetes Nocardiopsis dassonvillei MAD08. Appl Microbiol Biotechnol, 2009; 83 (3), 435-445.
44.    Sharmakumar M, Arunagirinathan N, Anand D, Girivasan KP, Baskar M, Rameshkumar MR, Elumalai A, Kumar MR. In-vitro study on Antibacterial and Anticancer activities of marine Sponge Clathria frondera Associated Bacteria. Research J. Pharm and Tech. 2020. 13(8); 3753-3758
45.    Setyowati EP, Sylvia UTP, Hertiani T, Samara O. Bioactivity of Fungi Trichoderma reesei Associated with Sponges Stylissa flabelliformis Collected from National Park West Bali, Indonesia. Journal of Biological Sciences, 2017; 17 (8), 362-368.
46.    Setyowati EP, Sylvia UTP, Purwantiningsih, Samara O. Antimicrobial activity and Identification of fungus associated Stylissa flabelliformis sponge collected from Menjangan Island West Bali National Park, Indonesia. Indonesian Journal of Pharmacy, 2018; 29 (2), 66-73.
47.    Setyowati EP, Purwantiningsih, Purwantini I. In vitro selectivity and apoptosis mechanism of cytotoxic of ethyl acetate extract from fungus Trichoderma reesei strain TV221 associated with marine sponge Stylissa flabelliformis. Journal of Applied Pharmaceutical Science, 2018; 8(9), 151-157.
48.    Suman T, Kaleeswaran B, Titus I, Dam RS, Krishnan P. Antibacterial Activity of Pseudoporea purpurea – A Marine Sponge from Andaman. Research J. Pharm and Tech. 2013. 6(5); 544-549
49.    Sutejo IR, Putri H, Meiyanto E. The Selectivity of Ethanolic Extract of Buah Makassar (Brucea javanica) on In Vitro Study of Metastatic Breast Cancer. Journal of Agromedicine and Medical Sciences, 2016; 2(1), 1-5.
50.    Torres VR, Encinar JA, Lopez, MH, Sanchez AP, Galiano V, Catalan EB, Micol V. An Updated Review on Marine Anticancer Compounds: The Use of Virtuallyy screening for the Discovery of Small-Molecule Cancer Drugs. Molecules, 2017; 22, 1-37.

Recomonded Articles:

Author(s): Lovely Joylen Castelino, Anoop Narayanan V, Swapnil Dylan Fernandes, Pankaj Kumar, Sandeep D S

DOI: 10.5958/0974-360X.2018.00590.5         Access: Open Access Read More

Author(s): Raj Kumar R, Praveen D, Palani Shanmugasundaram

DOI: 10.5958/0974-360X.2016.00164.5         Access: Open Access Read More

Author(s): K. Vanmathi Selvi, S.Aruna, S. Rajeshkumar

DOI: 10.5958/0974-360X.2017.00332.8         Access: Open Access Read More

Author(s): Monisha N.

DOI: Not Available         Access: Open Access Read More

Author(s): Subhashree Singh, Bhaskar Chandra Sahoo, Subrat Kumar Kar, Ambika Sahoo, Sanghamitra Nayak, Basudeba Kar, Suprava Sahoo

DOI: 10.5958/0974-360X.2020.00834.3         Access: Open Access Read More

Author(s): Iroko Emamuzo Matthew, Khokhlenkova Natalya

DOI: 10.5958/0974-360X.2016.00109.8         Access: Open Access Read More

Author(s): Hisham A. Abbas, Fathy M. Serry, Eman M. EL-Masry

DOI: Not Available         Access: Open Access Read More

Author(s): M. K. Shelar, M. J. Patil, S. S. Bhujbal

DOI: 10.5958/0974-360X.2019.00216.6         Access: Open Access Read More

Author(s): A. Rajasekaran, R. Arivukkarasu, Joyce Mathew P, Raja Bonagiri, Ramadasu Partha Saradhi

DOI: Not Available         Access: Open Access Read More

Author(s): Arun Chauhan, Sarabjeet Kaur, Rahul Singh

DOI: 10.5958/0974-360X.2018.00939.3         Access: Open Access Read More

Author(s): Fatima Grace Xavier, Anbarasan Balu, Shanmuganathan Seetharaman, Akila Lakshmikandhan, Arul Amutha Elizabeth Lawrence

DOI: 10.5958/0974-360X.2019.00786.8         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags