Author(s): Shefali Sharma, Rupali Chaudhary, Rajan Rolta, Nitin Sharma, Anuradha Sourirajan, Kamal Dev, Vikas Kumar

Email(s): vikaskmr59@gmail.com

DOI: 10.5958/0974-360X.2021.00057.3   

Address: Shefali Sharma1, Rupali Chaudhary1, Rajan Rolta1, Nitin Sharma2, Anuradha Sourirajan1, Kamal Dev1, Vikas Kumar1*
1Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, PO Sultanpur, Distt. Solan-173229 (HP), India.
2Department of Biotechnology, Chandigarh College of Technology, CGC Landran, 140307, Mohali, Punjab, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 1,     Year - 2021


ABSTRACT:
Rhododendron arboreum (family- Ericaceae) is one among the medicinally and economically important plant species from Himalayan region. Aim of present study was to study the effect of solvent on extraction yield, phytochemicals and antioxidant potential of various parts of R. arboretum. Extract of various solvents (n-hexane, methanol and water) were prepared using cold maceration method. Spectrophotometric method was used to quantify total phenolic and flavonoid content of different extracts. DPPH, FRAP and ABTS method was used to evaluate antioxidant activity of different extracts of various parts of R. arboreum. Among all solvent extract of different part of R. arboretum, highest extraction yield was observed in aqueous extract of leaf (10.78%) followed by methanolic extract of flowers (10.42%). Methanolic extract of flower showed higher amount of TPC (107.46±8.74 mg/g GAE) and TFC (254.26±29.33 mg/g RE). DPPH, FRAP and ABTS assay showed lowest IC50 value in flower extracts (methanolic and water) and hence validating the utilization of flower of this tree as source of natural antioxidants.


Cite this article:
Shefali Sharma, Rupali Chaudhary, Rajan Rolta, Nitin Sharma, Anuradha Sourirajan, Kamal Dev, Vikas Kumar. Effect of solvent on yield, phytochemicals and in vitro antioxidant potential of Rhododendron arboreum. Research J. Pharm. and Tech. 2021; 14(1):311-316. doi: 10.5958/0974-360X.2021.00057.3

Cite(Electronic):
Shefali Sharma, Rupali Chaudhary, Rajan Rolta, Nitin Sharma, Anuradha Sourirajan, Kamal Dev, Vikas Kumar. Effect of solvent on yield, phytochemicals and in vitro antioxidant potential of Rhododendron arboreum. Research J. Pharm. and Tech. 2021; 14(1):311-316. doi: 10.5958/0974-360X.2021.00057.3   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-1-57


REFERENCES:
1.    Laloo RC, Kharlukhi L, Jeeva S, Mishra BP. Status of medicinal plants in the disturbed and the undisturbed sacred forests of Meghalaya, northeast India: population structure and regeneration efficacy of some important species. Current Science. 2006: 225-32.
2.    Haq F, Ahmad H, Ullah R, Iqbal Z. Species diversity and ethno botanical classes of the flora of Allai valley district Battagram Pakistan. International Journal of Plant Sciences. 2012; 2 (4):111-23.
3.    Uniyal SK, Singh KN, Jamwal P, Lal B. Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya. Journal of Ethnobiology and Ethnomedicine. 2006: 2 (1):14.
4.    Sharma P, Samant SS. Diversity, distribution and indigenous uses of medicinal plants in Parbati Valley of Kullu district in Himachal Pradesh, Northwestern Himalaya. Asian Journal of Advanced Basic Sciences 2014; 2:77-98.
5.    Zhasa NN, Hazarika P, Tripathi YC. Indigenous knowledge on utilization of plant biodiversity for treatment and cure of diseases of human beings in Nagaland, India, A case study. International Research Journal of Biological Sciences 2015; 4 (4): 89-106.
6.    Paul A, Khan ML, Das AK. Utilization of rhododendrons by Monpas in western Arunachal Pradesh, India. Journal American Rhododendron Society. 2010; 64 (2): 81-4.
7.    Yadav VK, Deoli J, Rawat L, Adhikari BS. Traditional Uses of Medicinal Tree Species in Renuka Forest Division, Western Himalaya. Asian Pacific Journal of Health Sciences, ISSN. 2014: 2349-0659.
8.    Sharma N, Sharma UK, Gupta AP, Sinha AK. Simultaneous determination of epicatechin, syringic acid, quercetin-3-O-galactoside and quercitrin in the leaves of Rhododendron species by using a validated HPTLC method. Journal of Food Composition and Analysis. 2010; 23(3): 214-9.
9.    Swaroop A, Gupta AP, Sinha AK. Simultaneous determination of quercetin, rutin and coumaric acid in flowers of Rhododendron arboreum by HPTLC. Chromatographia. 2005; 62 (11-12): 649.
10.    Hariharan V, Rangaswami S. Chemical investigation of the bark of Rhododendron arboreum Sm. Current Science. 1966; 35(15): 390-1.
11.    Orwa C, Mutua A, Kindt R, Jamnadass R, Anthony S. Agroforestree Database: a tree reference and selection guide version 4.0. World Agroforestry Centre, Kenya. 2009;15.
12.    Rangaswami S, Sambamurthy K. Chemical examination of the leaves of Rhododendron nilagiricum Zenk. Proceedings Mathematical Sciences. 1959; 50: 366-73.
13.    Verma N, Singh AP, Amresh G, Sahu PK, Rao CV. Protective effect of ethyl acetate fraction of Rhododendron arboreum flowers against carbon tetrachloride-induced hepatotoxicity in experimental models. Indian Journal of Pharmacology. 2011; 43: 291.
14.    Rangaswami S, Sambamurthy K. Crystalline chemical components of the flowers of Rhododendron nilagiricum Zenk. In Proceedings of the Indian Academy of Sciences-Section A. Springer India.1960: 516, pp. 322-327.
15.    Swaroop A, Gupta AP, Sinha AK. Simultaneous determination of quercetin, rutin and coumaric acid in flowers of Rhododendron arboreum by HPTLC. Chromatographia. 2005; 62 (11-12): 649.
16.    Kumar V, Dev K, Sourirajan A, Khosla PK. Comparative antioxidant potential of bark and leaves of Terminalia arjuna (Roxb) Wight & Arn from Himachal Pradesh. International Journal of Pharmaceutical and Phytopharmacological Research. 2016; 6(1): 27-33.
17.    Kumar V, Sharma N, Sourirajan A, Khosla PK, Dev K. Comparative evaluation of antimicrobial and antioxidant potential of ethanolic extract and its fractions of bark and leaves of Terminalia arjuna from north-western Himalayas, India. Journal of Traditional and Complementary Medicine. 2018; 8(1):100-6.
18.    Chandel SR, Kumar V, Guleria S, Sharma N, Sourirajan A, Khosla PK, Baumler DJ, Dev K. Sequential Fractionation by Organic Solvents Enhances the Antioxidant and Antibacterial Activity of Ethanolic Extracts of Fruits and Leaves of Terminalia bellerica from North Western Himalayas, India. Pharmacognosy Journal. 2019;11(1).
19.    Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in Enzymology. Academic Press.1999 Vol. 299, pp. 152-178.
20.    Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 1999; 64(4): 555-9.
21.    Barros L, Baptista P, Ferreira IC. Effect of Lactarius piperatus fruiting body maturity stage on antioxidant activity measured by several biochemical assays. Food and Chemical TOXICOLOGY. 2007; 45(9): 1731-7.
22.    Rolta R, Sharma A, Kumar V, Kumar Chauhan P, Sourirajan A, Dev K. Traditional Medicinal Plants of Higher Altitude of Himachal Pradesh as Functional Food Ingredient Cum Food Preservative and Bioavailability Enhancer of Antifungal Antibiotics. Available at SSRN 3299285. 2018(a); 11.
23.    Rolta R, Sharma A, Kumar V, Sourirajan A, Baumler DJ, Dev K. Methanolic Extracts of the Rhizome of R. emodi Act as Bioenhancer of Antibiotics against Bacteria and Fungi and Antioxidant Potential. Medicinal Plant Research. 2018(b); 28: 8.
24.    Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry. 1996; 239(1): 70-6.
25.    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999; 26(9-10): 1231-7.
26.    Razak MF, Yong PK, Shah ZM, Abdullah LC, Yee SS, Yaw TC. The Effect of Varying Solvent Polarity on Extraction Yield of Orthosiphon stamineus leaves. Journal of Applied Sciences. 2012; 12(11):1207-10.
27.    Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis. 2014; 22(3): 296-302.
28.    Bhandari L, Rajbhandari M. Isolation of quercetin from flower petals, estimation of total phenolic, total flavonoid and antioxidant activity of the different parts of Rhododendron arboreum smith. Scientific World. 2014; 12(12): 34-40.
29.    Kashyap P, ANAND S. Phytochemical and GC-MS analysis of Rhododendron arboreum flowers. International Journal of Farm Sciences. 2017; 6(4): 145-51.
30.    Acharya K, Giri S, Biswas G. Comparative study of antioxidant activity and nitric oxide synthase activation property of different extracts from Rhododendron arboreum flower. International Journal of PharmTech Research. 2011; 3(2):757-62.
31.    Mani TT, Badami S, Kumar A, Suresh B, Singh VK, Govil JN. Antioxidant and hepatoprotective activities of Rhododendron arboretum leaf. Phytopharmacol Therap Valu. 2008; 3:3.
32.    Prakash D, Upadhyay G, Singh BN, Dhakarey R, Kumar S, Singh KK. Free-radical scavenging activities of Himalayan rhododendrons. Current Science (00113891). 2007; 92(4).
33.    Kumar D, Arora S, Kumar M, Thakur MK, Singh AP. Membrane stabilizing activity and antioxidant effect of Sida cordata (Burm F.) Bioss and Rhododendron arboreum Sm. leaves. International Journal of Pharmaceutical Sciences and Research. 2014; 5(1): 165-70.
34.    Painuli S, Joshi S, Bhardwaj A, Meena RC, Misra K, Rai N, Kumar N. In vitro antioxidant and anticancer activities of leaf extracts of Rhododendron arboreum and Rhododendron campanulatum from Uttarakhand region of India. Pharmacognosy Magazine. 2018; 14(57): 294.
35.    Kashyap P, Anand S, Thakur A. Evaluation of antioxidant and antimicrobial activity of Rhododendron arboreum flowers extract. International Journal of Food and Fermentation Technology. 2017; 7(1): 123-8.

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