Vivek Keshri, K. Rajeshwar Dutt
Vivek Keshri1*, Dr. K. Rajeshwar Dutt2
1Research Scholar, Department of Pharmacy, Shri Jagdish Prasad Jhabarmal Tibrewala University, Jhunjhun, Rajasthan, India.
2Guide, Department of Pharmacy, Shri Jagdish Prasad Jhabarmal Tibrewala University, Jhunjhun, Rajasthan, India.
Volume - 14,
Issue - 1,
Year - 2021
The present work was targeted to determine the total phenolic and flavonoidal content along with the determination of anti-oxidant behavior of methanolic root extract of Heliotropium indicum linn. The result reveals that 400 ± 7.31 mg Gal1ic acid equiva1ent of phenolic content and 540 ±59.34 mg quercetin equiva1ents of flavonoidal content was present in per gram dry weight of plant extract. The antioxidant data obtained from the work reveal that as the concentration increases the radical scavenging activity also increased. At 1000 µg/ml it has shown 91% inhibition. From this study, It was understood that anti-oxidant behavior of root extract is associated with total phenolic and flavonoidal content of extract.
Cite this article:
Vivek Keshri, K. Rajeshwar Dutt. Inhibitory effect of Phenolic and Flavonoidal content of H. indicum Root Extract on 1,1- diphenyl-2-picrylhydrazyl radicals. Research J. Pharm. and Tech. 2021; 14(1):235-238. doi: 10.5958/0974-360X.2021.00041.X
Vivek Keshri, K. Rajeshwar Dutt. Inhibitory effect of Phenolic and Flavonoidal content of H. indicum Root Extract on 1,1- diphenyl-2-picrylhydrazyl radicals. Research J. Pharm. and Tech. 2021; 14(1):235-238. doi: 10.5958/0974-360X.2021.00041.X Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-1-41
1. Raman, W, and Khalid, A. right Nin. Studies on free radicals, antioxidants, and co-factors. Clinical Interventions in Aging. (2007). , 2(2), 219-36.
2. Juránek, I, and Bezek, Š. S. Controversy of Free Radical Hypothesis: Reactive Oxygen Species- Cause or Consequence of Tissue Injury? Gen Physiol Biophys (2005), 24, 263-278 263. (2005). , 24, 2263-278.
3. Stocker R, Keaney JF Role of oxidative modifications in atherosclerosis. Physiol. Rev. 2004; 84: 1381-1478.
4. Grassi D, Desideri G, Tiberti S, Ferri C Oxidative stress endothelial dysfunction and prevention of cardiovascular diseases. Agro Food Industry Ii-tech. 2009; 20: 76-79.
5. Devasagayam TPA, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS and Lele RD Free radicals and antioxidants in human health: Current status and future prospects. J. Assoc. Physicians India2004; 52:794–804.
6. Lee, D, Lim, B, Lee, Y. K, and Yang, H. C. Effects of hydrogen peroxide (H2O2) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines. Cell Biol Toxicol 22. (2006). , 22, 39-46.
7. Manolagas, S, and Parfitt, A. What old means to bone. Trends Endocrinol Metab. 2010;Epub (2010). Mar 11., 21(6), 369-3744.
8. Mate, J. M, and Perez-gomez, C. Nunez de Castro I. Antioxidant Enzymes and Human Diseases. Clinical Biochemistry. (1999). , 32(8), 595-603.
9. Rao, L. Lycopene and the Prevention of Osteoporosis. AV R, editor. Scotland: Caledonia Science; (2006).
10. Willcox, J, Ash, S, and Catignani, G. Antioxidants and prevention of chronic disease. Crit Rev Food Sci Nutr. (2004). , 44, 275-95.
11. Rao, A, and Carotenoids, L. G R. and human health (Review). Pharmacological Research. (2007). , 55(3), 207-16.
12. Dash KG, Abdullah SM. A Review on Heliotropium indicum L. (Boraginaceae). International Journal of Pharmaceutical Sciences and Research. 2013; 4(4):1253-1258.
13. Das Kumar Ajit, Dutta KB, Sharma DG. Medicinal plants used by different tribes of Cachar district, Assam. Indian Journal of Traditional Knowledge. 2008; 7(3):446-454.
14. Kumar Pandi P, Ayyanar M, Ignacimuthu S. Medicinal plants used by Malasar tribes of Coimbatore district, Tamil Nadu. Indian Journal of Traditional Knowledge.2007; 6(4):579-582.
15. Michalak A Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol. J. Environ. Stud.2006; 15: 523-5.
16. McDonald S, Prenzler PD, Autolovich M, Robards K Phenolic content and antioxidant activity of olive extracts. Food Chem. 2001; 73:73-84.
17. Dewanto V, Wu X, Adom KK, Liu RH Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of Agricultural and Food Chemistry 2002; 50:3010–3014.
18. Sreejayan N and Rao MNA Free radical scavenging activity of curcuminoids. Drug Res. 1996; 46: 169-178.
19. Rice-Evans CA, Miller NJ, Bolwell PG, Gramley PM, Pradham JB The relative antioxidant activities of plant derived polyphenolic flavonoids. Free Radical Res. 1995; 22:375-383.
20. Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I Study on the inhibitory effect of tannins and flavonoids against the 1, 1-diphenyl-2-picrylhydrazyl radicals. Biochem Pharmacol 1998; 56: 213-222.
21. Obayed Ullah M, Sultana S, Haque A, Tasmin S., (2009), Eur J Sci Res, Antimicrobial, cytotoxic and antioxidant activity of Centella asiatica, 3, 2009, 260-264.