Author(s): Saurabh Singh, Shivangi Ahuja, Lakshmi Thangjam, Neha Kumari, Daljeet Singh Dhanjal, Chirag Chopra, Reena Singh

Email(s): reena.19408@lpu.co.in

DOI: 10.5958/0974-360X.2021.00038.X   

Address: Saurabh Singh1, Shivangi Ahuja1, Lakshmi Thangjam1, Neha Kumari1, Daljeet Singh Dhanjal1, Chirag Chopra1, Reena Singh1*
1School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 1,     Year - 2021


ABSTRACT:
Proteases, the enzymes that digest proteins, are present in all organism and play an essential role in proliferation and differentiation. They are crucial for different applications in industries like detergent, food, laundry and pharmaceutical. Proteases from bacteria and fungi have emerged as a salvation to the industrial sectors. In the presented work, protease producing Aspergillus terreus was grown from the field soil of Birring village, Jalandhar. Further, Aspergillus terreus was screened for growth on different substrates like Okara, Broken Wheat, Chick Pea and Black Gram. After, substrate selection, the solid-state fermentation was found to be an efficient method for enzyme production. Upon characterisation, the crude enzyme was found to be extracellular. The optimum enzyme activity was at pH of 10.0 and a temperature of 55oC. Also, Mn2+enhanced the enzyme activity. Thus, the low-cost substrate and effective protease activity, can make this enzyme as ideal candidate for various industrial applications.


Cite this article:
Saurabh Singh, Shivangi Ahuja, Lakshmi Thangjam, Neha Kumari, Daljeet Singh Dhanjal, Chirag Chopra, Reena Singh. Comparative Evaluation of Protease Production from Okara, Broken wheat, Chickpea and black gram by the soil isolate of Aspergillus terreus. Research J. Pharm. and Tech. 2021; 14(1):219-224. doi: 10.5958/0974-360X.2021.00038.X

Cite(Electronic):
Saurabh Singh, Shivangi Ahuja, Lakshmi Thangjam, Neha Kumari, Daljeet Singh Dhanjal, Chirag Chopra, Reena Singh. Comparative Evaluation of Protease Production from Okara, Broken wheat, Chickpea and black gram by the soil isolate of Aspergillus terreus. Research J. Pharm. and Tech. 2021; 14(1):219-224. doi: 10.5958/0974-360X.2021.00038.X   Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-1-38


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