Author(s):
Mohammed K. Abbas, Duaa A. Kadhum, Adnan Kamel Shabeeb, Saif Ali Mohammed
Email(s):
husamshaft@gmail.com
DOI:
10.5958/0974-360X.2020.00779.9 
Address:
Mohammed K. Abbas1, Duaa A. Kadhum2, Adnan Kamel Shabeeb3*, Saif Ali Mohammed4
1Department of Public Health Techniques, Baqubah-Technical Institute, Middle Technical University, Baghdad, Iraq.
3Department of Medical Laboratories Techniques, Kut -Technical Institute, Middle Technical University, Iraq.
2,4Department of Medical Laboratories Techniques, Baqubah- Technical Institute, Middle Technical University, Baghdad, Iraq.
*Corresponding Author
Published In:
Volume - 13,
Issue - 9,
Year - 2020
ABSTRACT:
Purpose: To investigates the synergistic efficacy of synergistic antibiotic combinations effect on P. aeruginosa strains and evaluates the typical antimicrobial effects for Ciprofloxacin and Streptomycin with Cefotaxime on P. aeruginosa strains. Methods: Pseudomonas Isolation Agar (PIA) has been employed to isolate a clinical P. aeruginosa. For minimum inhibitory concentration (MIC) of Cefotaxime, Ciprofloxacin, and Streptomycin, the combination antimicrobial effect of these antibiotics also determined based on half-chess board method. Standard strain of E. coli MM294 E. and E.coli HB101 has been used as control. Results: The clinical samples were resistant to Cefixime Ciprofloxacin by rates of 80%, 5%, respectively. Correspondingly, MICs of the Cefixime, Ciprofloxacin, and Streptomycin were between 32-1024µg/ml, 2–128µg/ml, 128-1224µg/ml respectively. The results of Ciprofloxacin-Streptomycin combination with Cefotaxime showed a synergic effect and a significant reduction in the MIC value at a ratio (1: 3) for 20 strains with percentage of 95.23%, while the other combinations have depicted different fallouts based on synergic, addition, indifference and Antagonism. Conclusions: Cefotaxime with Streptomycin can weaken antibiotic resistance mechanisms of P.aeruginosa strains. It can make the Bacterium, as mentioned above, more susceptible to weaker concentrations of antibiotics.
Cite this article:
Mohammed K. Abbas, Duaa A. Kadhum, Adnan Kamel Shabeeb, Saif Ali Mohammed. Combination Effect of Ciprofloxacin and Streptomycin with Cefotaxime against Multi-Drug Resistant Pseudomonas aeruginosa from Different Clinical Samples. Research J. Pharm. and Tech. 2020; 13(9):4403-4408. doi: 10.5958/0974-360X.2020.00779.9
Cite(Electronic):
Mohammed K. Abbas, Duaa A. Kadhum, Adnan Kamel Shabeeb, Saif Ali Mohammed. Combination Effect of Ciprofloxacin and Streptomycin with Cefotaxime against Multi-Drug Resistant Pseudomonas aeruginosa from Different Clinical Samples. Research J. Pharm. and Tech. 2020; 13(9):4403-4408. doi: 10.5958/0974-360X.2020.00779.9 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-9-67
REFERENCES:
1. Hassan, J.F. Al-Safar, M.A. Abdul Rheman, T.R. The Role of DNA Gyrase (gyrA) in Ciprofloxacin-Resistant Locally Isolates Pseudomonas aeruginosa in Al-Khadhmiya Teaching Hospital Baghdad [dissertation], Iraq, J Pure Appl. Microbiol., 2019; 13(1):499-503.
2. Suleyman G, Alangaden G, Bardossy AC. The role of environmental contamination in the transmission of nosocomial pathogens and healthcare-associated infections. Current infectious disease reports. 2018 Jun 1; 20(6):12.
3. Martinez JA, Cobos-Trigueros N, Soriano A, Almela M, Ortega M, Marco F, Pitart C, Sterzik H, Lopez J, Mensa J. Influence of empiric therapy with a β-lactam alone or combined with an aminoglycoside on prognosis of bacteremia due to gram-negative microorganisms. Antimicrobial agents and chemotherapy. 2010 Sep 1; 54(9):3590-6.
4. Rani A, Jain S, Dureja P, Kumar R, Kumar A. Synergistic interaction between synthetic and natural products: a promising tool for the development of environmentally safe potent antimicrobial agents. World Appl Sci J. 2009; 5(Special Issue for Environment): 59-63.
5. Casey JR, Pichichero ME. Metaanalysis of short course antibiotic treatment for group a streptococcal tonsillopharyngitis. The Pediatric infectious disease journal. 2005 Oct 1; 24(10):909-17.
6. Lambert PA. Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. Journal of the royal society of medicine. 2002; 95(Suppl 41):22.
7. Kamatou GP, Viljoen AM, Van Vuuren SF, Van Zyl RL. In vitro evidence of antimicrobial synergy between Salvia chamelaeagnea and Leonotis leonurus. South African Journal of Botany. 2006 Nov 1; 72(4):634-6.
8. Rammo RN. Evaluation of some Antibiotics in Combination Activity Against Isolates of Staphylococcus aureus and Pseudomonas aeruginosa. Journal of Al-Nahrain University-Science. 2014; 17(4):174-9.
9. Shree DB, Telagi VT, Jain SN, Gowda RV, Sampath AS, KG SK, Gopal VN. Evaluation of in vitro synergy between amipicillin and kanamycin against staphylococcus aureus. Journal of Drug Delivery and Therapeutics. 2012 Jul 15; 2(4).
10. Garcia LS, editor. Clinical microbiology procedures handbook. American Society for Microbiology Press; 2010 Aug 1.503-642p.
11. National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial susceptibility testing; 15th informational supplement M100-S15. National Committee for Clinical Laboratory Standards. Wayne, PA. 2007
12. Mandal S, Mandal MD, Pal NK. Evaluation of combination effect of ciprofloxacin and cefazolin against Salmonella enteric serovar typhi isolates by in vitro methods. Calicut Med J. 2004; 2(2): e2.
13. Chung PY, Navaratnam P, Chung LY. Synergistic antimicrobial activity between pentacyclic triterpenoids and antibiotics against Staphylococcus aureus strains. Annals of Clinical Microbiology and Antimicrobials. 2011 Dec; 10(1):25.
14. Vincent JL. Nosocomial infections in adult intensive-care units. The lancet. 2003 Jun 14; 361(9374):2068-77.
15. Munita JM, Arias CA. Mechanisms of antibiotic resistance. Microbiology spectrum. 2016 Apr; 4(2).
16. Hamza, S.J,.Al-Zubaidy, S.A.H. and Al-Amir, L.A.K. Effect of sub inhibitory concentrations of antibiotics on adherance of pseudomonas aeruginosa. Iraqi Journal of Biotechnology. 2006; 5(1):99-111.
17. Shawar RM, MacLeod DL, Garber RL, Burns JL, Stapp JR, Clausen CR, Tanaka SK. Activities of tobramycin and six other antibiotics against Pseudomonas aeruginosa isolates from patients with cystic fibrosis. Antimicrobial agents and chemotherapy. 1999 Dec 1; 43(12):2877-80.
18. Hope, Arian, Sharifi. The effect of methanolic extract of oak, wild pistachio and onion on the biofilm formation of Pseudomonas aeruginosa. Provides knowledge. 2017 Jan 15; 21 (10): 999-1012. In this case, we have the following:
19. Braide W, Madu LC, Adeleye SA, Korie MC, Akobondu CI. Prevalence of extended spectrum beta lactamase producing Escherichia coli and Pseudomonas aeruginosa isolated from clinical samples. International Journal of Science. 2018; 7:89-93.
20. Abdullah, R.M.; Samaan, S.F. and AL-Shwaikh, A.M. study the effect of antibiotic combination of beta – Lactam and amino glycoside with another group of antibiotics and their synergism effect. Journal of Arab Board of Health specializatio. 2010; Vol.11, No.1.
21. Chang CM, Lee HC, Lee NY, Huang GC, Lee IW, Ko WC. Cefotaxime‐ciprofloxacin combination therapy for nontyphoid Salmonella bacteremia and paravertebral abscess after failure of monotherapy. Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy. 2006 Nov; 26(11):1671-4.
22. Al Marjani MF, Al-Ammar M, Kadhem EQ. Occurrence of ESBL and MBL genes in Pseudomonas aeruginosa and Acinetobacter baumannii isolated from Baghdad, Iraq. Int J Cur Res. 2013; 5:2482-6.
23. Fadhel R. Inhibition of Biofilm Production of Pseudomonas aeruginosa Isolated from Patients with Diabetic Foot Ulcer Grade II [dissertation] University of Mustansiriyha; 2017 Oct. 125 p.
24. Chapman JS, Georgopapadakou NH. Routes of quinolone permeation in Escherichia coli. Antimicrobial agents and chemotherapy. 1988 Apr 1; 32(4):438-42.
25. Nishino T. The synergic effects of quinolones and oral cephem antibiotics on Serratia marcescens. Journal of Antimicrobial Chemotherapy. 1996 Nov 1; 38(5):771-6.
26. Chang C, Chuang Y, Wu J, Wang L, Lee H, Lee N, Chen H, Wu C, Ko W. In vitro combination of cefotaxime and ciprofloxacin against non-typhoid salmonellae. International journal of antimicrobial agents. 2009; 33(6):593-4.
27. Ali MK, Sultana S. Antimicrobial sensitivity patterns of Salmonella typhi in children. Bangladesh J Med Sci 2016; 15:416-8.
28. Hasan B, Nahar SG, Akter L, Saleh AA. Antimicrobial sensitivity pattern of Salmonella typhi isolated from blood culture in a referral hospital. Bangladesh J Med Microbiol 2011; 5:16-20.