Author(s):
Bokov D.O., Nizamova L.A., Morokhina S.L., Marakhova A.I., Bobkova N.V., Sergunova E.V., Kovaleva T.Yu., Balobanova N.P., Prostodusheva T.V., Klyukina E.S., Chevidaev V.V., Samylina I.A., Lazareva N.B., Krasnyuk I.I.
Email(s):
fmmsu@mail.ru
DOI:
10.5958/0974-360X.2020.00772.6 
Address:
Bokov D.O.1,2*, Nizamova L.A.3, Morokhina S.L.4, Marakhova A.I.5, Bobkova N.V.1, Sergunova E.V.1, Kovaleva T.Yu.1, Balobanova N.P.1, Prostodusheva T.V.1, Klyukina E.S.1, Chevidaev V.V.1, Samylina I.A.1, Lazareva N.B.1, Krasnyuk I.I.1
1Sechenov First Moscow State Medical University, 8, Trubetskaya St., bldg. 2, 119991, Russian Federation.
2Federal Research Center of Nutrition, Biotechnology and Food Safety, 2/14, Ustyinsky pr., Moscow,109240, Russian Federation.
3Bashkir State Medical University, 3, Lenina street, Ufa, 450008, Republic of Bashkortostan, Russian Federation.
4Financial University under the Government of the Russian Federation (Financial University), 55, Leningradsky Prospekt, Moscow, 125057, Russian Federation.
5Рeoples’ Friendship University of Russia (RUDN University), 6, Miklukho-Maklaya Street, Moscow, 117198, Russian Federation.
*Corresponding Author
Published In:
Volume - 13,
Issue - 9,
Year - 2020
ABSTRACT:
Objective: Russian Oregano (Origanum vulgare L.) and Turkish oregano (Origanum onites L.) are pharmacopoeial plants that are used for producing medicinal plant raw materials (crude herbal drugs). The aim of the study was to determine the composition and content of biologically active substances in Origanum crude herbal drugs. Materials and methods: Complex of modern physicochemical methods was used in pharmacognostical research. Thin-layer chromatography, gas-liquid chromatography was applied for the determination of crude herbal drugs composition. The content of total flavonoids in terms of cynaroside was determined by differential UV spectrophotometry, total tannins content – by redox titration, essential oil – by distillation. Microscopic analysis was performed on a Biomed-C2 microscope with 5×, 10× eyepieces, 4×, 10× and 40× objectives, photographies – were taken with a Canon Power Short SX 210 IS digital camera.
Results: Thymol is a dominant component in essential oil. Two species contain a cynaroside (flavonoid). The content of essential oil in O. vulgare and O. onites herb was 0.12±0.03% and 2.60±0.02% respectively; the total flavonoids in terms of cinaroside – 0.98±0.02% and 1.25±0.01%; total polyphenols – 11.54±0.35% and 19.43± 0.72% (condensed polyphenols precipitated by gelatin – 4.94±0.15% and 13.62±0.21%); ascorbic acid – 0.35± 0.05% and 0.40±0.06%. Existing ones were modified and a number of new unified procedures were developed, allowing qualitative and quantitative analysis of phenolic compounds in O. vulgare and O. onites herb. A complex of anatomical and diagnostic features (the structure of the stomatal complex, simple hairs, ethereal-oil glands) was established to carry out CHD identification. Conclusion: Composition and content of the polyphenolic complex and hydrophilic biologically active compounds of Origanum vulgare L. and Origanum onites L. crude herbal drugs (CHD) were determined. The obtained data will be used to improve the existing regulatory documentation for Origanum crude herbal drugs standardization.
Cite this article:
Bokov D.O., Nizamova L.A., Morokhina S.L., Marakhova A.I., Bobkova N.V., Sergunova E.V., Kovaleva T.Yu., Balobanova N.P., Prostodusheva T.V., Klyukina E.S., Chevidaev V.V., Samylina I.A., Lazareva N.B., Krasnyuk I.I. Pharmacognostic studies of Origanum L. species Medicinal plant raw materials. Research J. Pharm. and Tech. 2020; 13(9):4365-4372. doi: 10.5958/0974-360X.2020.00772.6
Cite(Electronic):
Bokov D.O., Nizamova L.A., Morokhina S.L., Marakhova A.I., Bobkova N.V., Sergunova E.V., Kovaleva T.Yu., Balobanova N.P., Prostodusheva T.V., Klyukina E.S., Chevidaev V.V., Samylina I.A., Lazareva N.B., Krasnyuk I.I. Pharmacognostic studies of Origanum L. species Medicinal plant raw materials. Research J. Pharm. and Tech. 2020; 13(9):4365-4372. doi: 10.5958/0974-360X.2020.00772.6 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-9-60
REFERENCES:
1. Bokov DO, Morokhina SL. Pharmacotherapeutic action and use in practical medicine of Oregano vulgare L. herb. Medicine and Health: Proceedings of the international. Scientific. konf. Chita: Publishing young scientist. 2012; 52-59.
2. Plantarium. The determinant of plants on-line. Open Atlas of Plants and Lichens in Russia and Adjacent Countries. Origanum onites L. (Lamiaceae family) http://www.plantarium.ru/page/image/id/586110.html [Last accessed on 2019 Jan 7].
3. Plantarium. The determinant of plants on-line. Open Atlas of Plants and Lichens in Russia and Adjacent Countries. Origanum vulgare L. (Lamiaceae family) http://www.plantarium.ru/page/image/id/503865.html [Last accessed on 2019 Jan 7].
4. Plant Resources of the USSR: Flowering plants, their chemical composition, the use; Families Hippuridaceae - Lobeliaceae. St. Petersburg.: Science. 1991; 200 p.
5. Bokov DO, Morokhina SL. Prospects for the use of Turkish oregano (Origanum onites L.) herb material as a basis for the development of functional foods. Current trends in agriculture: the Collection of the I International Internet Conference. Kazan: Kazan University Publishing.2012; 32-38.
6. Chishti S, Kaloo ZA, Sultan P. Medicinal importance of genus Origanum: A review. Journal of Pharmacognosy and Phytotherapy. 2013; 5(10): 170-177.
7. Alyakin AA, Efremov AA, Kachin SV. Fraction compounds of essential oil of Origanum vulgare L. from Krasnoyarsk region. Chemistry of plant raw materials. 2010; 1: 99-104.
8. Kurkin VA. Pharmacognosy: a textbook for pharmaceutical universities (faculties). 2nd ed. rev. and add. Samara. 2007; 1239p.
9. Werker E, Putievky E, Ravid U. The essential oils and glandular hais in different chemotipes of Origanum vulgare L. Ann. Bot. 1985; 6 (55): 793-801.
10. Mirovich VM, Konenkina TA, Fedoseyeva GM, Golovnih NN. Study of the qualitative composition of the Oregano vulgare L. essential oil growing in Eastern Siberia. Chemistry of plant raw materials. 2008; 2: 61-64.
11. Bokov D.O., Morokhina S.L., Popov D.M. (2015). Phytochemical Study of Essential Oil from Turkish Oregano (Origanum onites L.) by gas chromatography/mass spectrometry. Pharmaceutical Chemistry Journal. 2015; 49(4): 259-267.
12. Bendini A, Toschi TG, Lercker G. Antioxidant activity of oregano (Origanum vulgare L.) leaves. Italian Journal of Food Science. 2002; 14(1): 17-24.
13. State register of medicines http://www.grls.rosminzdrav.ru/grls.aspx
14. Bokov DO, Morokhina SL, Tereshina NS. Turkish oregano (Origanum onites L.) herb as a basis for creating new homeopathic drugs: approaches to standardization of medicinal plant materials. Homeopathic yearbook. Proceedings of the XXIII Moscow International Homeopathic Conference “Development of a homeopathic method in modern medicine”. M.: Techpolygraphcentr. 2013; 167-170.
15. Wnag Y, Wang L, Liu L, Liu JX, Ma SX, Chen G. Anti-tumor activity of Origanum oil in vitro [J]. Chinese Journal of Microecology. 2010; 12: 015.
16. Baser KHC. The Turkish Origanum species. Oregano, the Genera Origanum and Lippia, Ed., Kintzios SE, London and New York, Taylor and Francis, 2002;109-126.
17. Swagatika Pradhan, Smaranika Pattnaik. Phytochemical Screening of Components Present Floral Essential oil of an Indigenous Variety of Lantana camara, Linn (Verbenaceae). Res. J. Pharmacognosy and Phytochem. 2017; 9(4): 203-209.
18. The State Pharmacopoeia of the USSR XI edition. Common methods of analysis. Herbal drugs. Moscow, Medicine. 1989. Issue 2. 400p.
19. Sbayou H, Boumaza A, Hilali A, Amghar S. Chemical composition and antibacterial and antioxidant activities of Thymus satureioides Coss. essential oil. Int J Pharm Pharm Sci 2016; 8(10): 183-187.
20. HAS Hussein M, Tkachenko K, Nkomo M, Mudau, F. Essential oil composition of Artemisia vulgaris grown in Egypt. Int J Pharm Pharm Sci 2016; 8(9): 120-123.
21. Akbar A, Kuanar A, Sandeep I, Kar B, Singh S, Mohanty S, Patnaik J, Nayak S. GC-MS analysis of essential oil of some high drug yielding genotypes of turmeric (Curcuma longa L.). Int J Pharm Pharm Sci 2015; 7(9): 35-40.
22. Bokov DO, Samylina IA, Nikolov SD. Amaryllidaceae alkaloids GC/MS analysis in Galanthus woronowii and Galanthus nivalis of Russian origin. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2016; 7(6): 1625-9.
23. Bokov DO, Samylina IA. Comparison of the chemical compositions of Galanthus woronowii Losinsk. and Galanthus nivalis L. homeopathic mother tinctures by gas chromatography with mass-selective detection. Pharmaceutical Chemistry Journal. 2017 50(10), 659-667.
24. The State Pharmacopoeia of the USSR XI edition. Common methods of analysis. Moscow, Medicine. 1987. Issue 1. 336 p.
25. Muthukumar N, Sami Veerappa N. Powder Microscopic analysis on Leaf, Petiole, Stem and Root on Thespesia populnea (L.) Soland ex Correa. Res. J. Pharmacognosy and Phytochem. 2019; 11(3):189-192.
26. Mahajan KG, Tamilvanan S, Sawarkar HS, Thenge RR, Adhao VS, Gangane PS. Preliminary Phytochemical and Anti-pyretic Screening of Crude extract of the leaf of Clerodendrum colebrookianum. Research J. Pharmacognosy and Phytochemistry 2009; 1(3): 191-193.
27. Malarkodi Velraj, Armstrong, Ravichandran, Jeyakumari, Hemalatha, Vijayalakshmi, Srikanth.J. Pharmacognostical and Preliminary Phytochemical Studies on the Stem Bark of Soymida febrifuga (Roxb). Research J. Pharmacognosy and Phytochemistry 2009; 1(3) 213-216.
28. Saravanan J, Shariff WR, Joshi Narasimhachar H, Varatharajan R, Joshi VG, Karigar Asif A. Preliminary Pharmacognostical and Phytochemical Studies of Leaves of Hemigraphis colorata. Research J. Pharmacognosy and Phytochemistry 2010; 2(1): 15-17.
29. Yogesh Shivhare, Priya Singh, K. K. Pate, Prashant Soni. Preliminary Phytochemical Screening and Antioxidant Activity of Petroleum Ether Extract of Trichosanthes dioica Roxb (Fruits). Research J. Pharmacognosy and Phytochemistry 2010; 2(2): 155-158.
30. Samylina IA, Antonova NP, Rudakova IP. A study to design a pharmacopoeial assay of the content of tanning agents in the raw medicinal plant materials. Pharmacy. 2009; 6: 3-6.
31. Wadkar KA, Magdum CS. Evaluation of Total Phenolic Content, Flavonoid Content and Antioxidant Activity of Stem Bark of Careya arborea Roxb. Research J. Pharmacognosy and Phytochemistry 2010; 2(1): 49-51.
32. Preeti Tiwari, Rakesh K. Patel. Total Phenolics and Flavonoids and Antioxidant Potential of Draksharishta Prepared by Traditional and Modern Methods. Research J. Pharmacognosy and Phytochemistry 2012; 4(5): 244-249.
33. Sheela D, Cheenickal M. Total phenolics and flavonoids among the selected species of Syzygium, Gaertn. Res. J. Pharmacognosy and Phytochem. 2017; 9(2): 101-104.
34. Kavitha Sagar, Soma Aneesha, Pooja Uppin, Gowthami. Phytochemical Studies and Quantification of total content of Phenols, Tannins and Flavonoids in selected endangered plant species. Res. J. Pharmacognosy and Phytochem. 2018; 10(4): 277-281.
35. Rindita, Hanani E, Saidah N. Pharmacognostical, Preliminary Phytochemical Evaluation and Flavonoids Content of Paederia foetida Linn. Res. J. Pharmacognosy and Phytochem. 2019; 11(2):81-86.
36. Kakorin PA, Tereshkina OI, Ramenskaya GV. Potential biological activity and chemical composition of Caragana jubata (Pall.) Poir. Pharmaceutical chemistry journal. 2018; 52(6): 531-535.
37. Smyslova OA, Bokov DO, Potanina OG, Litvinova TM, Samylina IA. Development and validation of spectrophotometric procedure for quantitative determination of flavonoid content used to control the quality of mixture herbal product. Journal of advanced pharmaceutical technology and research. 2019; 10(4), 155-162
38. Bokov DO. Standardization of snowdrop (Galanthus L.) herbal pharmaceutical substances by ultraviolet-spectrophotometry. Asian J Pharm Clin Res. 2008; 11(10): 207-211.
39. Kakorin PA, Perova IB, Rybakova ED, Éller KI, Ramenskaya GV, Pavlova LA, Teselkin YO. Biologically Active Compounds in Aqueous Extracts of Caragana jubata (Pall.) Poir. Pharmaceutical chemistry journal. 2018. 51(11); 1014-1020.
40. Bokov D.O., Samylina, I.A. Identification of flavonoids in homeopathic mother tinctures of Galanthus woronowii Losinsk. and Galanthus nivalis L. by ultra-performance liquid chromatography with photodiode arrays and tandem quadrupole mass-selective detectors. Pharmaceutical Chemistry Journal. 2016. 50(7), 458-464.
41. Guseinov MD, Bobkova NV, Svistunov AA, Tarasov VV, Bokov DO, Sergunova EV. Flavonoids in Passiflora incarnata L. Dry Extract of Russian Origin. Pharmacog J. 2019;11(5):1143-7.
42. Sorokina AA. Use of spectrophotometry in the analysis of the production prototypes of raw medicinal plant material. Pharmacy. 2012; 4: 43-44.
43. The requirements for medicines from plant materials [Electronic resource]: Good Manufacturing Practices (Good Manufacturing Practice). Mode of access: http://www.gmpua.com/GACP/Herbals.htm/ (date accessed: 21.04.2018).