Author(s):
Umamaheswari. R, S. Kothai
Email(s):
aarthi.r90@gmail.com
DOI:
10.5958/0974-360X.2020.00770.2
Address:
Umamaheswari. R*1, S. Kothai*1
1*Research Scholar, Department of Chemistry, Ethiraj College for Women, Chennai, Tamilnadu-600008, India.
2*Principal, Ethiraj College for Women, Chennai, Tamilnadu-600008, India.
*Corresponding Author
Published In:
Volume - 13,
Issue - 9,
Year - 2020
ABSTRACT:
The aim of the study was to evaluate the green synthesized copper nanoparticles loaded microsponges for the better wound healing activity. Being an excellent antimicrobial and antioxidant agent, proven already, now the loaded microsponges also checked for its in- vitro drug release study in which it fits best into the korsemeyer-peppas kinetic model. It was subjected to cytotoxicity test against Vero cell lines using MTT assay. It showed IC50 value of 125 µg. Wound healing activity of this microsponge was analyzed through in-vitro scratch assay in which it possessed a potential wound healing property.
Cite this article:
Umamaheswari. R, S. Kothai. Effectiveness of Copper nanoparticles loaded microsponges on Drug release study, Cytotoxicity and Wound healing activity. Research J. Pharm. and Tech 2020; 13(9):4357-4360. doi: 10.5958/0974-360X.2020.00770.2
Cite(Electronic):
Umamaheswari. R, S. Kothai. Effectiveness of Copper nanoparticles loaded microsponges on Drug release study, Cytotoxicity and Wound healing activity. Research J. Pharm. and Tech 2020; 13(9):4357-4360. doi: 10.5958/0974-360X.2020.00770.2 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-9-58
REFERENCES:
1. Aloorkar NH et al. Microsponges as Innovative Drug Delivery Systems. International Journal of Pharmaceutical Sciences and Nanotechnology. 2012; 5(1):1597–1606.
2. Resmi DS et al. Formulation and Evaluation of Topical Econazole Nitrate Microsponge Loaded Hydrogel. Indian Journal of Pharmacy and Pharmaceutical Research. Human. 2018; 12(1): 27-64.
3. Zhou Y et al. Antibacterial activities of gold and silver nanoparticles against Escherichia coli and bacillus Calmette-Guerin. Journal of Nanobiotechnology. 2012; 10(19): 1-9.
4. Travan A et al. Noncytotoxic silver nanoparticle polysaccharide nanocomposites with antimicrobial activity. Biomacromolecules. 2009; 10(6): 1429-1435.
5. Pinto RJ et al. Antibacterial activity of nanocomposites of silver and bacterial or vegetable cellulose fiber. Acta Biomaterilia. 2009; 5(6): 2279-2289.
6. Syed Ahamed Hussain I, Jaisankar V. An eco-friendly synthesis, Characterisation and antibacterial applications of novel almond Gum- poly (Acrylamide) based hydrogel silver Nanocomposite. Polymer Testing. 2017; 62: 154-161.
7. Bailong Jao et al. Copper- nanoparticle-embedded hydrogel for killing bacteria and promoting wound healing with photothermal therapy, Journal of Material Chemistry B, 2019; 7: 2534-2548.
8. Nathan BM et al. Impaired Wound Healing. Clinics in Dermatology. 2007; 25: 19–25.
9. Nwomeh BC et al. Physiology of the Chronic Wound. Clinics in Plastic Surgery. 1998; 25: 341–356.
10. Brem H et al. Healing of Diabetic Foot Ulcers and Pressure Ulcers with Human Skin Equivalents. Archives of Surgery. 2000; 135: 627–634.
11. Tang J. A small peptide with potential ability to promote wound healing. PLoS One. 2014; 9(3).
12. Schmidt C. Biological studies on Brazilian plants used in wound healing. Journal of Ethnopharmacology. 2009;122(3): 523–32.
13. Todaro, G J et al. The initiation of cell division in a contact-inhibited mammalian cell line. Journal of Cellular Physiology. 1965; 66, 325–333.
14. Liang CC et al. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols. 2007; 2(2): 329–33.
15. Kothai S and Umamaheswari R, Evaluation of Antimicrobial and Antioxidant Activity of Green Synthesized Copper Nanoparticles from the Leaf Extract of Hibiscus Rosa-Sinensis, International Journal of Green and Herbal Chemistry. 2018; Vol.7(4), 834-842.
16. Kothai S, Umamaheswari R. Formulation and Evaluation of Copper Nanoparticles Loaded Microsponges. International Journal of Pharmaceutical Science and Drug Research. 2019; 11(4): 141-146.
17. Edsman K et al. Rheological evaluation of poloxamer as an in situ gel for ophthalmic use. European Journal of Pharmaceutical sciences. 1998; 16: 105-112.
18. Ganguly S, Dash AK. A novel in situ gel for sustained drug delivery and targeting. International Journal of Pharmaceutics. 2004; 276: 83-92.
19. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983; 65(1-2):55–63.
20. Balakrishnan S et al. gold nanoparticle-conjugated quercetin inhibits epithelial-mesenchymal transition, angiogenesis and invasiveness via EGFR/VEGFR-2-mediated pathway in breast cancer, Wiley Cell Proliferation, 2016;49:678-697.