ABSTRACT:
Objectives of this study were to determine the antimotility and antispasmodic effects of both methanolic and aqueous extracts of Nephelium lappaceum on contractile response induced by acetylcholine and histamine and on isolated chicken ileum. Methanolic and aqueous extracts of N. lappaceum were prepared by using maceration technique. Graded doses of acetylcholine and histamine were used to trigger the contractile response. Atropine, mepyramine and N. lappaceum extracts were used as the antagonists to reduce the intestinal contractions. Concentration response curves in the absence and in the presence of both extracts were plotted separately. Half maximal effective concentration (EC50) in the presence of extracts were higher than EC50 in the absence of extracts. EC50 of acetylcholine in the presence of 4mg/mL and 8 mg/mL methanolic extracts were 1.59µg/mL and 1.71µg/mL respectively whereas EC50 of acetylcholine alone is 1.38µg/mL. In the presence of 2mg/mL and 4mg/mL of aqueous extracts, EC50 of acetylcholine also increased to 1.98 and 2.58µg/mL respectively. EC50 of acetylcholine in the presence of 2µg/mL atropine was the lowest which was 9.84µg/mL. EC50 of histamine alone is 0.93µg/mL. After addition of 1 mg/mL and 2mg/mL methanolic extracts as well as 2mg/mL and 4 mg/mL aqueous extracts, EC50 increased to 1.08, 1.24, 1.03 and 1.09µg/mL respectively. In the presence of 2 µg/mL mepyramine, EC50 of histamine is 1.55µg/mL. In conclusion, both methanolic and aqueous extracts showed antimotility and antispasmodic properties by blocking acetylcholine and histamine.
Cite this article:
Sasikala Chinnappan, Mogana R., Tan Xue Qin. In vitro Antimotility and Antispasmodic effects of Nephelium lappaceum on isolated chicken ileum. Research J. Pharm. and Tech 2020; 13(9):4346-4350. doi: 10.5958/0974-360X.2020.00768.4
Cite(Electronic):
Sasikala Chinnappan, Mogana R., Tan Xue Qin. In vitro Antimotility and Antispasmodic effects of Nephelium lappaceum on isolated chicken ileum. Research J. Pharm. and Tech 2020; 13(9):4346-4350. doi: 10.5958/0974-360X.2020.00768.4 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-9-56
REFERENCES:
1. Troeger C, Forouzanfar M, Rao PC, Khalil I, Brown A, Reiner Jr et al. Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015. The Lancet Infectious Diseases. 2017;17(9):909-48.
2. Joo-Perez R, Avendano-Arrazate CH, Sandoval-Esquivez A, Espinoza-Zaragoza S, Alonso-Baez M, Moreno-Martinez JL, et al. Alternancy Study on Rambutan (Nephelium lappaceum L.) Tree in Mexico. American Journal of Plant Sciences. 2016;8(01):40.
3. Blancke R. Tropical fruits and other edible plants of the world: An illustrated guide. Cornell University Press; 2016. p.19.
4. Orwa C, Mutua A, Kindt R, Jamnadass R, Simons A. Agroforestree database: a tree species reference and selection guide version 4.0. World Agroforestry Centre ICRAF, Nairobi, KE. 2009.
5. Kaushik G, Satya S, Khandelwal RK, Naik SN. Commonly consumed Indian plant food materials in the management of diabetes mellitus. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 2010;4(1):21-40.
6. Sukmandari NS, Dash GK, Jusof WH, Hanafi M. A Review on Nephelium lappaceum L. Research Journal of Pharmacy and Technology. 2017;10(8):2819-27.
7. Burkill IH. A dictionary of the economic products of the Malay Peninsula. A Dictionary of the Economic Products of the Malay Peninsula. 1966; p.2.
8. Mohamadi M, Maskooki AM, Mortazavi SA. Evaluation of Antioxidant Properties of Barberry Fruits Extracts using Maceration and Subcritical Water Extraction (SWE). World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering. 2012 ;6(9):699-703.
9. Urbina AV, Martin ML, Montero MJ, Carron R, Sevilla MA, Roman L. Antihistaminic activity of pulegone on the guinea‐pig ileum. Journal of pharmacy and pharmacology. 1990;42(4):295-6.
10. Saba AB, Oridupa OA. Pharmacological reactivity of isolated guinea pig ileum to ethanol leaf extracts of Amaranthus caudatus and Solanum melongena. Niger J Physiol Sci. 2012 ;27(1):73-8.
11. Doggrell SA. M1 and M2-muscarinic receptors in the epididymal half of the rat vas deferens. General pharmacology. 1986;17(2):239-41.
12. Yamamura Hi, Snyder Sh. Muscarinic cholinergic receptor binding in the longitudinal muscle of the guinea pig ileum with [3H] quinuclidinyl benzilate. Molecular Pharmacology. 1974;10(6):861-7.
13. Piercey MF, Ruwart MJ. Naloxone inhibits the anti‐diarrhoeal activity of loperamide. British journal of pharmacology. 1979;66(3):373-5.
14. Carter D, Champney M, Hwang B, Eglen RM. Characterization of a postjunctional 5-HT receptor mediating relaxation of guinea-pig isolated ileum. European journal of pharmacology. 1995;280(3):243-50.
15. Ehlert FJ. Contractile role of M2 and M3 muscarinic receptors in gastrointestinal, airway and urinary bladder smooth muscle. Life sciences. 2003;74(2-3):355-66.
16. Leurs R, Brozius MM, Smit MJ, Bast A, Timmerman H. Effects of histamine H1‐, H2‐and H3‐receptor selective drugs on the mechanical activity of guinea‐pig small and large intestine. British journal of pharmacology. 1991;102(1):179-85.
17. Fitzsimons CP, Monczor F, Fernandez N, Shayo C, Davio CA. Mepyramine, a histamine H1 receptor inverse agonist, binds preferentially to a G protein coupled form of the receptor and sequesters G protein. Journal of Biological Chemistry. 2004;279(33):34431-9.
18. Sukmandari NS, Dash GK, Jusof WH, Hanafi M. A Review on Nephelium lappaceum L. Research Journal of Pharmacy and Technology. 2017;10(8):2819-27.
19. Rojas A, Cruz S, Rauch V, Bye R, Linares E, Mata R. Spasmolytic potential of some plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders. Phytomedicine. 1995;2(1):51-5.
20. Youmans WB, Karstens AI, Aumann KW. Relation of the extrinsic nerves of the intestine to the inhibitory action of atropine and scopolamine on intestinal motility. Journal of Pharmacology and Experimental Therapeutics. 1943;77(3):266-73.
21. Umadevi I, Daniel M. Chemosystematics of the Sapindaceae. Feddes Repertorium. 1991;102(7‐8):607-12.
22. Capasso A, Pinto A, Mascolo N, Autore G, Capasso F. Reduction of agonist‐induced contractions of guinea‐pig isolated ileum by flavonoids. Phytotherapy Research. 1991;5(2):85-7.