Author(s): Raghad Helaliy, Fadi Alrouh, Saleh Trefi, Yaser Bitar


DOI: 10.5958/0974-360X.2020.00717.9   

Address: Raghad Helaliy1, Fadi Alrouh2, Saleh Trefi1, Yaser Bitar1
1Department of Pharmaceutical Chemistry and Quality Control-Faculty of Pharmacy, University of Aleppo-Syria.
2Department of Organic Chemistry -Faculty of Medicine-University of Al-Hawash-Syria.
*Corresponding Author

Published In:   Volume - 13,      Issue - 9,     Year - 2020

Objective: The objective of this study was developed and validated of a simple, rapid and accurate GC method for quantitative determination of thymol which considers as a chemical marker in thyme extract and in the final formulation by GC-FID chromatography. Method: thymol analysis was done by using menthol as internal standard, chromatographic conditions were TRB-5-625 capillary column and its specification 60m?0.25mm i.d0.25µm film thickness and nitrogen as a carrier gas at a flow rate of 0.6mL/min. The oven temperature was programmed between 185? and 235? for 16min. The injector and detector temperatures were maintained 290?. Detection was done by using flame ionization detector FID. Results: the developed method was validated according to ICH guidelines. The developed method was given accepted linearity which R>0.99 in the concentration range of 1-40mg/10ml of thymol, accuracy with mean recovery values between 98%-102%, precision with relative standard deviation of the calculated concentration was less than 2%. Conclusion: The results showed that the developed method was effective in determination of thymol in pharmaceutical forms and routine analysis in quality control laboratories simply.

Cite this article:
Raghad Helaliy, Fadi Alrouh, Saleh Trefi, Yaser Bitar. Determination Thymol in Thyme extract and its Pharmaceutical forms by using Gas Chromatography method. Research J. Pharm. and Tech 2020; 13(9):4055-4060. doi: 10.5958/0974-360X.2020.00717.9

Raghad Helaliy, Fadi Alrouh, Saleh Trefi, Yaser Bitar. Determination Thymol in Thyme extract and its Pharmaceutical forms by using Gas Chromatography method. Research J. Pharm. and Tech 2020; 13(9):4055-4060. doi: 10.5958/0974-360X.2020.00717.9   Available on:

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