Author(s):
Michelle C. do Rosario, Prativa Poddar, Swarnkumar Reddy, W. Jabez Osborne
Email(s):
jabez.vit@gmail.com
DOI:
10.5958/0974-360X.2020.00747.7 
Address:
Michelle C. do Rosario, Prativa Poddar, Swarnkumar Reddy, W. Jabez Osborne*
School of Biosciences and Technology, Vellore Institute of Technology, Vellore - 632014, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 13,
Issue - 9,
Year - 2020
ABSTRACT:
Endophytes are group of microbes that colonize in plants and other organisms which can be isolated readily. Seaweeds and its endophytes are reservoirs of several bioactive metabolites likes biosurfactants, antimicrobial compounds and several compounds with biological interests. The present study on investigation of biotechnological potential of endophytes from Turbinaria ornata. The presence of endophytes was initially confirmed by SEM analysis and further 7 bacterial strains were isolated. These strains were further screened for their ability in production of biosurfactants and bioactive compounds. The endophytes were tested biofilm formation. The endophytes were tested for heavy metal tolerance in which all the isolates showed resistance to heavy metals and VITJMP7 the most effective isolate which showed most desirable in antibacterial activity and biosurfactant production, exhibited high resistance to heavy metals up to 4000ppm. The isolate was further examined for the uptake of heavy metals by bacterial biofilm by column study. In which the Ca-Ag beads were used as biocarrier matrix for biofilm formation. The Ca-Ag with the developed biofilm showed significantly increased absorption of up to 20% compared to the beads without bioflim. The effective isolate (VITJMP7) was further molecularly characterised by 16S rRNA gene sequencing and was identified to be the closest neighbour of Cobetia amphilecti (99.59% similarity). In addition, Turbinaria ornata extracts were also evaluated for their antibacterial properties against three pathogens, the methanolic extract showed better activity on all pathogens.
Cite this article:
Michelle C. do Rosario, Prativa Poddar, Swarnkumar Reddy, W. Jabez Osborne. Biotechnological Applications of Turbinaria ornata and its Endophytes. Research J. Pharm. and Tech 2020; 13(9):4231-4238. doi: 10.5958/0974-360X.2020.00747.7
Cite(Electronic):
Michelle C. do Rosario, Prativa Poddar, Swarnkumar Reddy, W. Jabez Osborne. Biotechnological Applications of Turbinaria ornata and its Endophytes. Research J. Pharm. and Tech 2020; 13(9):4231-4238. doi: 10.5958/0974-360X.2020.00747.7 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-9-35
REFERENCE:
1. Domettila, C., Joselin, J., and Jeeva, S. (2013). Phytochemical analysis on some south Indian seaweeds. Journal of Chemical and Pharmaceutical Research, 5(4).
2. Roy S, Anantharaman P, Biochemical Compositions of Seaweeds Collected from Olaikuda and Vadakkadu, Rameshwaram, Southeast Coast of India. J Marine Sci Res Dev, 2017, 7: 240
3. Rekha M. Gupta, Prathamesh S. Kale, Madhuri L. Rathi and Nikhil N. Jadhav, Isolation, characterization and identification of endophytic bacteria by 16S rRNA partial sequencing technique from roots and leaves of Prosopis cineraria, Asian Journal of Plant Science and Research, 2015, 5(6), PP. 36-43.
4. Fergusson JE. The heavy elements: Chemistry, environmental impact and health effects. Oxford: Pergamin press, 1990, 382-399.
5. Nicholson, F. A., Smith, S. R., Alloway, B. 2003. An inventory of heavy metals imputs to agricultural soils in England and Wales. Sci. Total Environ. 311: 205–219.
6. Brar S.K., Verma M., Surampalli R., Misra K., Tyagi R., Meunier N., Blais J. Bioremediation of hazardous wastes—A review. Pract. Period. Hazard. Toxic Radioact. Waste Manag. 2006; 10:59–72. doi: 10.1061/(ASCE)1090-025X(2006)10:2(59).
7. P. Kaaria, V.Matiru, J. Wakibia, C. Bii, and M. Ndung u, Antimicrobial Activity of Marine Algae Associated Endophytes and Epiphytes from the Kenya Coast, International Journal Of Current Microbiology And Applies Sciences, Volume 4 Number 8 (2015) pp. 17-22.
8. Lubobi SF, Matunda C, Kumar V and Omboki B, Isolation of Bioactive Secondary Metabolites from Seaweeds Amphiroa anceps against Chicken Meat Associated Pathogens, Journal of Antimicrobial Agents, 2016, 2: 113.
9. Chatterjee S, Mukherjee A, Sarkar A, Roy P (2012) Bioremediation of lead by lead-resistant microorganisms, isolated from industrial sam- ple. doi: 10.4236/abb.2012.33041
10. Sherman, N., Cappuccino, J.G. (1992). Biochemical activities of microorganisms. In: Microbiology, A Laboratory Manual. The Benjamin / Cummings Publishing Co. California, USA.
11. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Bio Evol 24:1596–1599. doi:10.1093/molbev/msm092
12. Al-Rub, F. A. A., El-Naas, M.., Benyahia, F., and Ashour, I. (2004). Biosorption of nickel on blank alginate beads, free and immobilized algal cells. Process Biochemistry, 39(11), 1767–1773.
13. Barquilha, C. E. R., Cossich, E. S., Tavares, C. R. G., and Silva, E. A. (2017). Biosorption of nickel (II) and copper(II) ions in batch and fixed-bed columns by free and immobilized marine algae Sargassum sp. Journal of Cleaner Production, 150, 58–64.
14. Santhanam shanmughapriya, aseer manilal, sugathan sujith, joseph selvin, george seghal kiran, kalimuthusamy Natarajaseenivasan, Antimicrobial activity of seaweeds extracts against multiresistant pathogens, Annals Of Microbiology, 2008, 58 (3) 535-541.
15. Bhagavathy, S., P. Sumathi, and I. Jancy Sherene Bell. "Green algae Chlorococcum humicola-a new source of bioactive compounds with antimicrobial activity." Asian Pacific Journal of Tropical Biomedicine 1, no. 1 (2011): S1-S7.
16. Sriram, Muthu Irulappan, Shanmugakani Gayathiri, Ulaganathan Gnanaselvi, Paulraj Stanly Jenifer, Subramanian Mohan Raj, and Sangiliyandi Gurunathan. "Novel lipopeptide biosurfactant produced by hydrocarbon degrading and heavy metal tolerant bacterium Escherichia fergusonii KLU01 as a potential tool for bioremediation." Bioresource technology 102, no. 19 (2011): 9291-9295.
17. Acharya, A., Joshi, D. R., Shrestha, K., and Bhatta, D. R. (2012). Isolation and screening of thermophilic cellulolytic bacteria from compost piles. Scientific world, 10(10),43-46. http://dx.doi.org/10.3126/sw.v10i10.6861
18. Gowd, S. Srinivasa, and Pradip K. Govil. "Distribution of heavy metals in surface water of Ranipet industrial area in Tamil Nadu, India." Environmental monitoring and assessment 136, no. 1-3 (2008): 197-207.
19. Carrillo PG, Mardaraz C, Pitta-Alvarez SJ, Giulietti AM (1996) Isolation and selection of biosurfactant-producing bacteria. World J Microbiol Biotechnol 12:82- 84.
20. Ibanez, Juan Patricio, and Yoshiaki Umetsu. "Potential of protonated alginate beads for heavy metals uptake." Hydrometallurgy 64, no. 2 (2002): 89-99.
21. Bodour AA, Miller-Maier R (1998) Application of a modified drop collapse technique for surfactant quantification and screening of biosurfactant-producing microorganisms. J Microbiol Methods 32: 273-280.
22. Thavasi R, Sharma S, Jayalakshmi S (2011) Evaluation of Screening Methods for the Isolation of Biosurfactant Producing Marine Bacteria. J Pet Environ Biotechnol, 2011.
23. Walter V, Syldatk C, Hausmann R (2010) Screening concepts for the isolation of biosurfactant producing microorganisms. In: Biosurfactants: advances in Experimental Medicine and Biology (Sen R, Ed.), Springer-Verlag, Heidelberg,Vol. 672 pp 1-13.
24. Elansary, Hosam O., Mohamed ZM Salem, Nader A. Ashmawy, Kowiyou Yessoufou, and Ahmed AA El-Settawy. "In vitro antibacterial, antifungal and antioxidant activities of Eucalyptus spp. leaf extracts related to phenolic composition." Natural product research 31, no. 24 (2017): 2927-2930.
25. Ibrahim, Wael M. "Biosorption of heavy metal ions from aqueous solution by red macroalgae." Journal of Hazardous Materials 192, no. 3 (2011): 1827-1835.