ABSTRACT:
Aim- A simple, exceptionally cost-effective, extremely accurate, quite precise and highly reproducible method was developed and validated for simultaneous estimation of Tizanidine and Nimesulide in bulk and Pharmaceutical formulation. Method-The separation has been done on Reverse Phase C18, 250X4.6 mm, 5µ column (40°C temperature). The mobile phase contain acetonitrile and water (ACN:WATER) (60:40).pH 4.5with ortho phosphoric acid(OPA), the flow rate was set at 1.0ml /min, and UV detection at 305nm , mobile phase was sonicated for 25 min. before use. Result- The retention times were obtained at 6.309min.and 2.492min. for Nimesulide and Tizanidine respectively. With accuracy of less than 2%, LOQ for Nimesulide and Tizanidine were found as 29.75µg/ml and 0.9649µg/ml respectively similarly LOD was 9.79µg/ml and 0.3184µg/ml. Precision % RSD for intraday and interday were found as 0.2 and 0.64 for Tizanidine , 0.68 and 0.63 for Nimesulide. Linearity was found in the range of 10-50µg/ml and 0.2-10µg/ml for Nimesulide and Tizanidine respectively. The method was found robust for changes in the flow rate, ratio of mobile phase and detection wavelength. Conclusion- The method was found to be satisfactory and can be used successfully for determination of Tizanidine and Nimesulide simultaneously in bulk and pharmaceutical dosage form.
Cite this article:
Bharatee Chaudhari, Kartika Daniel. A Validated RP-HPLC Method for Simultaneous Estimation of Tizanidine and Nimesulide in Bulk and Pharmaceutical Formulation. Research J. Pharm. and Tech 2020; 13(9):4207-4212. doi: 10.5958/0974-360X.2020.00743.X
Cite(Electronic):
Bharatee Chaudhari, Kartika Daniel. A Validated RP-HPLC Method for Simultaneous Estimation of Tizanidine and Nimesulide in Bulk and Pharmaceutical Formulation. Research J. Pharm. and Tech 2020; 13(9):4207-4212. doi: 10.5958/0974-360X.2020.00743.X Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-9-31
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