Maria Matoetina Suprijono, Hidayat Sujuti, Dikdik Kurnia, Simon Bambang Widjanarko
Maria Matoetina Suprijono1,2*, Hidayat Sujuti3, Dikdik Kurnia4, Simon Bambang Widjanarko5
1Department of Food Technology, Faculty of Agricultural Technology-Widya Mandala Catholic University Surabaya, Jl. Dinoyo 42-44 Surabaya 60265 Indonesia.
2Postgraduate Program in Agriculture Science, Major in Agricultural Product Technology, Brawijaya University Malang, Indonesia, Jl. Veteran, Malang, East Java, Indonesia 65145.
3Faculty of Medicine-Brawijaya University Malang, Indonesia, Jl. Veteran, Malang, East Java, Indonesia 65145.
4Department of Chemistry, Faculty of Mathematics and Natural Sciences-Padjajaran University Bandung, Indonesia, Jl. Raya Bandung Sumedang KM 21 Hegarmanah, Jatinangor, Sumedang, Indonesia 45363.
5Department of Food Science and Technology, Faculty of Agricultural Technology-Brawijaya University, Malang, Indonesia, Jl. Veteran, Malang, East Java, Indonesia 65145.
Volume - 13,
Issue - 9,
Year - 2020
Many studies done in Red Fruit/RF as an antioxidant source, but there were some controversies because of the abundant fruit variety and growing place. Most researches focus on Red Fruit oil/RFO. In this work, we analyzed the antioxidant activity of Short-Red RF methanol/ME and ethyl acetic extracts/EE with multiple assays, then evaluated the relationship between phenolic content and antioxidant activity then phenolic effectiveness and antioxidant capacity. Total phenolic content/TPC and antioxidant activities (TAA, DPPH, ABTS, RP) were determined. The flavonoid identification was made using LCMS. RACI and PAC were calculated. EE showed significantly higher TPC (20.11µg GAE/ml extract) and scavenging activity for anion and cation radicals than ME (TPC= 9.88µg GAE/ml extract). The correlation between TAA and TPC (R2= 0.999) or TAA and radicals scavenging activity was significantly strong (R2= 0.997-1.000). EE had high and positive RACI (0.71). ME had higher PAC in TAA (1.82) and ABTS scavenging activity (4.75), whereas EE and ME had the same PAC in DPPH scavenging (2.08). The main phenolic identified in the extracts were taxifolin, quercetin, and quercetin-3-glucoside contributed in the radicals scavenging and also ferric ion reducing activity. Based on this study, Short-Red RF extracts are a potential anion and cation radical scavenger.
Cite this article:
Maria Matoetina Suprijono, Hidayat Sujuti, Dikdik Kurnia, Simon Bambang Widjanarko. Methanol and Ethyl Acetate Extracts of Red Fruit (Pandanus conoideus Lam.) Short-Red showed high of Phenolic and Radicals Scavenging Activities. Research J. Pharm. and Tech 2020; 13(9):4158-4164. doi: 10.5958/0974-360X.2020.00734.9
1. Santos-Zea L, Villela-Castrejón J, and Gutriérrez-Uribe JA. Bound Phenolics in Foods. In Merillon JM and Ramawat KG. Bioactive molecules in food, reference series in phytochemistry. Switzerland AG: Springer Nature. 2019; pp. 973-989
2. Sadsoeitoeboen MJ. Pandanaceae: aspek botani dan etnobotani dalam kehidupan suku Arfak di Irian Jaya. (Master Thesis). Bogor, Indonesia: Program Pascasarjana, Institut Pertanian Bogor. 1999.
3. Rohman A, Riyanto S, Yuniarti N, Saputra WR, Utami R, and Mulatsih W. Antioxidant activity, total phenolic, and total flavonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). International Food Research Journal. 2010; 17: 97-106
4. Sandhiutami NMD and Indrayani AAW. Uji aktivitas antioksidan, kandungan fenolik total, dan kandungan flavonoid total buah merah. Jurnal Ilmu Kefarmasian Indonesia. 2012; 10: 13-19
5. Handayani MDN, Soekarno PA, and Wanandi SI. Red fruit oil supplementation fails to prevent oxidative stress in rats. Universa Medicina. 2013; 32: 86-91
6. Nainggolan D. Aspek ekologis kultivar buah merah panjang (Pandanus conoideus Lam) di daerah dataran rendah Manokwari. (Research Report). Manokwari, Indonesia: Jurusan Kehutanan, Fakultas Pertanian, Universitas Cenderawasih. 2001.
7. Limbongan J, and Malik A. Peluang pengembangan buah merah (Pandanus conoideus Lamk.) di provinsi Papua. Jurnal Litbang Pertanian. 2009; 28: 134-141
8. Zebua LI, Supriatna J, Walujo EB, and Chikmawati T. Inventory of red fruit pandan (Pandanus conoideus Lamarck) in Papua, Indonesia. In Wickneswari Ratnam. Editor. New Trends and Challenges in Science and Technology: Proceedings the Second UKM-UI Joint Seminar Bangi, Selangor: Pusat Pengajian Biosains dan Bioteknologi, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia. 2009; pp. 360-363).
9. Murtiningrum, Sarungallo ZL, and Mawikere NL. The exploration and diversity of red fruit (Pandanus conoideus L.) from Papua based on its physical characteristics and chemical composition. Biodiversitas. 2012; 13: 124-129
10. Sarungallo ZL, Hariyadi P, Andarwulan N, and Purnomo EH. Characterization of chemical properties, lipid profile, total phenol, and tocopherol content of oils extracted from nine clones of red fruit (Pandanus conoideus). Kasetsart Journal (Natural Science). 2015; 49: 237-250
11. Sarungallo ZL, Murtiningrum, Santoso B, Roreng MK, and Latumahina. Nutrient content of three clones of red fruit (Pandanus conoideus) during the maturity development. International Food Research Journal. 2016; 23: 1217-1225
12. Taher A, and Repasi MS. Aktivitas antioksidan minyak buah merah dari kultivar Pandanus conoideus L yang berbeda. Jurnal Natural. 2009; 8: 58
13. Schlesier K, Harwat M, Bohm V, and Bitsch R. Assessment of antioxidant activity by using different in vitro Methods. Free Radical Research. 2002; 36: 177–187
14. Piluzza G, and Bullitta S. Correlations between phenolic content and antioxidant properties in twenty-four plant species of traditional ethnoveterinary use in the mediterranean area. Pharmaceutical Biology. 2011; 49: 240–247
15. Rebaya A, Belghith SI, Baghdikian B, Leddet VM, Mabrouki F, Olivier E, Cherif JK, and Ayadi MT. Total phenolic, total flavonoid, tannin content, and antioxidant capacity of Halimium halimifolium (Cistaceae). Journal of Applied Pharmaceutical Science. 2015; 5: 052-057
16. Büyüktuncel E, Porgali E, and Çolak C. Comparison of total phenolic content and total antioxidant activity in local red wines determined by spectrophotometric methods. Food and Nutrition Science. 2014; 5: 1660-1667
17. Achadiyani, Septiani L, Faried A, Bolly HMB, and Kurnia D. Role of the red fruit (Pandanus conoideus LAM) ethyl acetate fraction on the induction of apoptosis vs. downregulation of survival signaling pathways in cervical cancer cells. European Journal of Medicinal Plants. 2016; 13: 1-9
18. Salamah N, and Farahana L. Uji aktivitas antioksidan ekstrak etanol herba pegagan (Centella asiatica (L.) Urb) dengan metode fosfomolibdat. Pharmaçiana. 2014; 4: 23-30
19. Chanda S, and Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: an overview. African Journal of Microbiology Research. 2009; 3: 981-996
20. Ahmed D, Khan MM, and Saeed R. Comparative analysis of phenolics, flavonoids, and antioxidant and antibacterial potential of methanolic, hexanic, and aqueous extracts from Adiantum caudatum leaves. Antioxidants. 2015; 4: 394-409
21. Sun T, and Tanumihardjo SA. An integrated approach to evaluate food antioxidant capacity. Journal of Food Science. 2007; 72: R159-R165
22. Gorjanović SẐ, Alvarez-Suarez JM, Novaković MM, Pastor FT, Pezo L, Battino M, and Sužnjevic DẐ. Comparative analysis of antioxidant activity of honey of different floral sources using recently developed polarographic and various spectrophotometric assays. Journal of Food Composition and Analysis. 2013; 30: 13-18
23. Petrović M, Sužnjević DẐ, Pastor F, Veljović M, Pezo L, Antić M, and Gorjanović SẐ. Antioxidant capacity determination of complex samples and individual phenolics - multilateral approach. Combinatorial Chemistry High Throughput Screening. 2016; 19: 58-65
24. Salas PG, Soto AM, Carretero AS, and Gutiérrez AF. Phenolic-compound-extraction systems for fruit and vegetable samples. Molecules. 2010; 15: 8813-8826
25. Xu CC, Wang B, Pu YQ, Tao JS, and Zhang T. Advances in extraction and analysis of phenolic compounds from plant materials. Chinese Journal of Natural Medicines. 2017; 15: 0721-0731
26. Rajendran R, Hemachander R, Ezhilarasan T, Keerthana C, Saroja DL, Saichand KV, Abdullah MG. Phytochemical analysis and in-vitro antioxidant activity of Mimosa pudica Lin. leaves. Research Journal of Pharmacy and Technology. 2010; 3: 551-555
27. Roy A, and Saraf S. Antioxidant and antiulcer activities of an ethnomedicine: Alternanthera sessilis. Research Journal of Pharmacy and Technology. 2008; 1: 75-79
28. Faruk MO, Saha D, Chowdhury S, Paul S, and Kabir MG. In vitro antioxidant activity of methanolic aerial part extract of Mimosa pudica. Research Journal of Pharmacology and Pharmacodynamics. 2012; 4: 202-205
29. Sravanthi J, and Gangadhar RS. Quantification of antioxidant-phytochemical studies in Vitis vinifera L. varieties. Asian Journal of Pharmaceutical and Clinical Research. 2015; 8: 295-301
30. Salomi S, Muthukumaran P, and Umamaheshwari R. In-vitro antioxidant activity of Azima tetracantha leaves. Research Journal of Science and Technology. 2012; 4: 148-151
31. Raj AJ, and Dorairaj S. Phytochemical screening and in-vitro antioxidant activity of Cissus quadrangualris. Asian Journal of Research in Chemistry. 2011; 4: 876-878
32. Pryor WA. Cigarette smoke radicals and the role of free radicals in chemical carcinogenicity. Environmental Health Perspectives 1997; 105: S875-882
33. Elias J, Rajesh MG, Anish NP, Deepa P, and Jayan N. In vitro antioxidant activity of the methanolic extract of Simaruba glauca DC. Asian Journal of Research in Chemistry. 2010; 3: 312-315
34. Jayasri R, and Anuradha R. In vitro antioxidant and antibacterial activity of Boerhaavia diffusa. Research Journal Pharmacognosy and Phytochemistry. 2012; 4: 223-225
35. Suneetha B, Prasad KVSRG, Soumya BR, Nishantha PD, Kumar BS, and Rajaneekar D. Evaluation of in-vitro antioxidant activity of various extracts of Actinodaphne madraspatana leaves. Research Journal of Pharmacognosy and Phytochemistry. 2014; 6: 1-4
36. Mohite MS, Dr. Yadav AV, Raje VN, Mohite YG, and Thombre SA. Preliminary phytochemical investigation and in-vitro antioxidant activity of Borassus flabellifer Linn. Fruit Pulp. Research Journal of Pharmacognosy and Phytochemistry. 2012; 4: 326-329
37. Elias J, Rajesh MG, Anish NP, Manu MS, and Varkey IC. Terminalia chebula Retz. stem bark extract: a potent natural antioxidant. Asian Journal of Research in Chemistry. 2011; 4: 445-449
38. Suprijono MM, Sujuti H, Kurnia D, and Widjanarko SB. Computational study of antioxidant activity and bioavailability of Papua red fruit (Pandanus conoideus Lam.) flavonoids through docking toward human serum albumin. AIP Conference Proceeding 2019; 2108:020020 https://doi.org/10.1063/1.5109995
39. Topal F, Nar M, Gocer H, Kalin P, Kocyigit UM, Gülçin I, and Alwasel SH. Antioxidant activity of taxifolin: an activity-structure relationship. Journal of Enzyme Inhibition and Medicinal Chemistry. 2015; Early online: 1-10
40. Watak S, and Patil SS. Evaluation and Comparison of Antioxidant activity of Herbomineral Complex. Research Journal of Pharmacognosy and Phytochemistry. 2012; 4: 171-177
41. Shubina VS and Shatalin YV. Antioxidant and iron-chelating properties of taxifolin and its condensation product with glyoxylic acid. Journal of Food Science and Technology. 2017; 54: 1467-1475.