Author(s):
S Brintha, C Sivaraj, K Saraswathi, P Arumugam, L Jeyanthi Rebecca
Email(s):
shivaraj27@gmail.com
DOI:
10.5958/0974-360X.2020.00656.3 
Address:
S Brintha1, C Sivaraj2*, K Saraswathi2, P Arumugam2, L Jeyanthi Rebecca3
1Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selayur, Chennai 600073.
2Phytochemistry and Natural Products, Armats BIOTEK Training and Research Institute, Guindy, Chennai 600032.
3Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Selayur, Chennai 600073.
*Corresponding Author
Published In:
Volume - 13,
Issue - 8,
Year - 2020
ABSTRACT:
Hypericum hookerianum belongs to the family Hypericaceae, is a perennial shrub which has southern and eastern Asia as its native. In India, it is found in higher altitudes of Nilgiris, Khasi and jaintia hills of Himalayas and Palani hills of Western Ghats. The plant is used as folk medicine in various parts of the world and is used to treat an antidepressant and antiviral activity. The study is performed for the antioxidant and antibacterial activities of buds of Hypericum hookerianum. DPPH? radical scavenging, ABTS?+ radical cation scavenging, Fe3+ reducing power and phosphomolybdenum reduction assays were carried out for antioxidant activity. GC-MS analysis was carried out for the detection of volatile and semi-volatile compounds. The buds showed DPPH? radical inhibition of 86.16% at 120 µg/mL concentration and ABTS?+ radical cation inhibition of 83.65% at 30 µg/mL concentration. The phosphomolybednum reduction and Fe3+ reduction assays were showed 95.94% and 91.78% reduction at 120µg/mL concentration of the extract. Also, this extract exhibited good antibacterial activity against Escherichia coli. GC-MS analysis showed the presence of flavones (2H-1-Benzopyran-4,7-diol, 3,4-dihydro-2-phenyl- and 4H-1-Benzopyran-4-one, 2-(3,4-dimethoxyphenyl)-7-hydroxy-) which are responsible for antioxidant activity of the extract.
Cite this article:
S Brintha, C Sivaraj, K Saraswathi, P Arumugam, L Jeyanthi Rebecca. Antioxidant, Antibacterial activities and GC-MS analysis of Methanol extract of buds of Hypericum hookerianum Wight and Arnott. Research J. Pharm. and Tech. 2020; 13(8):3709-3714. doi: 10.5958/0974-360X.2020.00656.3
Cite(Electronic):
S Brintha, C Sivaraj, K Saraswathi, P Arumugam, L Jeyanthi Rebecca. Antioxidant, Antibacterial activities and GC-MS analysis of Methanol extract of buds of Hypericum hookerianum Wight and Arnott. Research J. Pharm. and Tech. 2020; 13(8):3709-3714. doi: 10.5958/0974-360X.2020.00656.3 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-8-29
REFERENCE:
1. Mahendrakumar M, Nirmalraj S, Ravikumar M, Bharath B, Seeni S, Perinbam K. Pharmacognostic, Phytochemical and Physicochemical Investigations of Hypericum hookerianum Wight & Arn.(Hypericaceae) of Palni Hills, India. Pharmacognosy Journal. 2017; 9(6).
2. Hypericum Depression Trial Study Group. Effect of Hypericum perforatum (St John's wort) in major depressive disorder: a randomized controlled trial. Jama. 2002 Apr 10; 287(14):1807-1814.
3. Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of agricultural and food chemistry. 1995 Jan;43(1):27-32.
4. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 1999 May 1; 269(2):337-341.
5. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958 Apr; 181(4617):1199.
6. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 1999 May 1;26(9-10):1231-1237.
7. Usman U, Amir M. Isolation of Secondary Metabolite Compounds and Antibacterial Activities Tests From Hexane Extract of Stem Bark Melochia umbellate (Houtt) Stapf var. degrabrata K. In2017 International Conference on Education and Technology (2017 ICEduTech) 2017 Nov 23. Atlantis Press.
8. Vardhini SP, Sivaraj C, Arumugam P, Himanshu Ranjan KT, Baskar M. Antioxidant, anticancer, antibacterial activities and GC-MS analysis of aqueous extract of pulps of Aegle marmelos (L.) Correa.
9. Sowndhararajan K, Kang SC. Free radical scavenging activity from different extracts of leaves of Bauhinia vahlii Wight & Arn. Saudi journal of biological sciences. 2013 Oct 1;20(4):319-25.
10. Vijayalakshmi M, Ruckmani K. Ferric reducing anti-oxidant power assay in plant extract. Bangladesh Journal of Pharmacology. 2016 Jun 1;11(3):570-2.
11. Phatak RS, Hendre AS. Total antioxidant capacity (TAC) of fresh leaves of Kalanchoe pinnata. Journal of Pharmacognosy and Phytochemistry. 2014 Jan 1;2(5).
12. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. Journal of food science and technology. 2011 Aug 1;48(4):412-22.
13. Kumar T, Jain V. Appraisal of total phenol, flavonoid contents, and antioxidant potential of folkloric Lannea coromandelica using in vitro and in vivo assays. Scientifica. 2015;2015.
14. Das B, Thirupathi P, Ravikanth B, Kumar RA, Sarma AV, Basha SJ. Isolation, synthesis, and bioactivity of homoiso flavonoids from Caesalpinia pulcherrima. Chemical and Pharmaceutical Bulletin. 2009 Oct 1;57(10):1139-41.
15. Kostrzewa-Susłow E, Janeczko T. Microbial transformations of 7-hydroxyflavanone. The Scientific World Journal. 2012;2012.
16. Kwon HM, Choi YJ, Jeong YJ, Kang SW, Kang IJ, Lim SS, Kang YH. Anti-inflammatory inhibition of endothelial cell adhesion molecule expression by flavone derivatives. Journal of agricultural and food chemistry. 2005 Jun 29;53(13):5150-7.
17. Hong H, Neamati N, Winslow HE, Christensen JL, Orr A, Pommier Y, Milne GW. Identification of HIV-1 integrase inhibitors based on a four-point pharmacophore. Antiviral Chemistry and Chemotherapy. 1998 Dec;9(6):461-72.