Author(s): Sukanth kumar Enmozhi, Vijayalakshmi Ganesan, Manigundan Kaari, Bharathi Selvaraj, Gopikrishnan Venugopal, Jerrine Joseph, Radhakrishnan Manikkam

Email(s): mrkactinos@gmail.com

DOI: 10.5958/0974-360X.2020.00646.0   

Address: Sukanth kumar Enmozhi1, Vijayalakshmi Ganesan2, Manigundan Kaari2, Bharathi Selvaraj3, Gopikrishnan Venugopal2, Jerrine Joseph2, Radhakrishnan Manikkam2
1School of Biotechnology and Genetic Engineering, Bharadhidasan University, Tiruchirappalli.
2Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai.
3Department of Microbiology, Sri Sankara Arts and Science College, Kanchipuram.
*Corresponding Author

Published In:   Volume - 13,      Issue - 8,     Year - 2020


ABSTRACT:
Isolation of actinobacteria from mangrove rhizosphere sediments has been investigated for their antimicrobial properties. Twenty eight actinobacterial strains were isolated from the rhizosphere sediments collected from Parangipettai, Tamil Nadu and Andaman mangrove ecosystems. Strain PPA12 from Parangipettai mangrove sediments, which showed promising antimicrobial activity in agar plug method, had been selected as potential strain. The PPA12 actinobacteria produced bioactive metabolite only by agar surface fermentation and not by submerged fermentation. The bioactive metabolite from the potent strain PPA12 that was produced by agar surface fermentation which was extracted using different solvents and the maximum extraction was with methanol. The minimum inhibitory concentration (MIC) of the methanol extract was found to be 125µg/ml against carbapenem resistant Klebsiella pneumoniae. Partial purification of the crude methanol extract was done by TLC and the bioautography showed that the active fractions with RF value of 0.288 and 0.55 have bioactivity. The physiochemical, molecular characterization and phylogenetic analysis of the potent strain showed that the actinobacteria PPA12 was found to be Streptomyces fumigatiscleroticus.


Cite this article:
Sukanth kumar Enmozhi, Vijayalakshmi Ganesan, Manigundan Kaari, Bharathi Selvaraj, Gopikrishnan Venugopal, Jerrine Joseph, Radhakrishnan Manikkam. Anti-infective potential of marine actinobacteria against carbapenem resistant Klebsiella pneumoniae ATCC13882. Research J. Pharm. and Tech. 2020; 13(8):3653-3660. doi: 10.5958/0974-360X.2020.00646.0


REFERENCES:
1.    Ibrahim AH, Samar YD,  Mostafa AF, Mohamed SK, Tobias AMG and Abdelmohsen UR. Natural Product Potential of the Genus Nocardiopsis. Marine Drugs. 2018; 16(5): 147.
2.    Manivasagan P, Kyong-Hwa K, Sivakumar KECY, Li-Chan, Hyun-Myung Oh and Se-Kwon Kim. Marine actinobacteria: An important source of bioactive natural products. Environ. Toxicol. Pharmacol. 2014; 38(1):172-188.
3.    Yu J, Liu Z, Qiu L, Xiaohui Q, Ying J and Beom SK. Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China. Asian Pacific J Tropical Biomed. 2015; 5(7):555-560.
4.    Elbendary AA, Hessain AM, El-Hariri MD, Seida AA, Moussa IM, Mubarak AS, Kabli SA, Hemeg HA and El Jakee JK. Isolation of antimicrobial producing Actinobacteria from soil samples. Saudi J. Biol. Sci. 2018; 25(1):44-46.
5.    Meletis G. Carbapenem resistance: overview of the problem and future perspectives. Therapeutic adv. Infectious Dis. 2016; 3(1):15-21.
6.    Sathiyaseelan K and Stella D. Isolation, identification and antimicrobial activity of marine actinomycetes isolated from parangipettai. Rec. Res. Sci. Tech. 2011; 3(9): 74-77.
7.    Radhakrishnan M, Balaji S and Balagurunathan R. Thermotolerant actinomycetes from the Himalayan Mountain-Antagonistic potential, characterization and identification of selected strains. Malaysian Appl. Bio. J. 2007; 36(1): 59-65.
8.    Mohamed H, Miloud B, Zohra F, Garcia-Arenzana JM, Veloso A and Rodriguez-Couto S. Isolation and Characterization of actinobacteria from Algerian Sahara soils with antimicrobial activities. Int. J. Mol. Cellular Med. 2017; 6(2):109-120.
9.    Radhakrishnan M, Pazhanimurugan R, Gopikrishnan V, Balagurunathan R and Vanaja Kumar. Streptomyces sp D 25 isolated from Thar Desert soil, Rajasthan producing pigmented antituberculosis compound only in solid culture. J. Pure Appl. Microbiol. 2014; 8(1): 333-337.
10.    Sathish KS and Kokati VBR.  In-vitro antimicrobial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus. Asian Pacific J. Tropical Biomed. 2012; 2(10):787-792.
11.    Eccleston GP, Brooks PR and Kurtboke DI. The occurrence of bioactive Micromonosporae in aquatic habitats of the sunshine coast in Australia. Marine Drug. 2008; 6243-6261.
12.    Bavya M, Mohanapriya P, Pazhani murugan R and Balagurunathan R. Potential bioactive compounds from marine actinomycetes against biofouling bacteria. Indian J. Geomarine Sci. 2011; 40(4): 578-582.
13.    Selvameenal L, Radakrishnan M and Balagurunathan R. Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening. Indian J. Pharma Sci. 2009; 71: 499 - 504.
14.    Augustine SK, Bhavsar SP and Kapadnis BP. A non-polyene antifungal antibiotic from Streptomyces albidoflavus PU23. J. Biosci. 2005; 30: 201 - 211.
15.    Balagurunathan R and Radhakrishnan M. Biotechnological, genetic engineering and nanotechnological potential of actinomycetes. 2010; p. 436 In D.K. Maheshwari, R.C. Dubey, and R. Saravanamuthu (ed.), Industrial Exploitation of Microorganisms. I.K. International Publishing House Pvt. Ltd, New Delhi.
16.    Shirling EB and Gottileb D. Methods for characterization of Streptomyces species. Int. J. Systematic Bacteriol. 1966; 6(3): 313 -340.
17.    Gothwal RK, Nigam VK, Mohan MK, Sasmal D and  Ghosh P. Screening of nitrogen fixers from rhizospheric bacterial isolates associated with important desert plants. Appl. Ecol. Environ Res. 2008; 6(2): 101-109.
18.    Takahashi YO and Omura S. Isolation of new actinomycete strains for the screening of new bioactive compounds. J Gen Applied Microbiol. 2003; 49: 141-154.
19.    Thompson JD, Higgins DG and Gibson TJ. Clustal W – improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22: 4673-4680.
20.    Boudjella H, Bouti K, Zitouni A,  Mathieu F,  Lebrihi A and Sabaou N. Taxonomy and chemical characterization of antibiotics of Streptosporangium Sg 10 isolated from a Saharan soil. Microbiol. Res. 2006; 161: 288-298.
21.    Felsenstein J.  Phylogenies and the Comparative Method. The American Naturalist. 1985; 125(1): 1-15.
22.    Berdy J. Thoughts and facts about antibiotics: where we are now and where we are heading. J. Antibiot. (Tokyo). 2012; 65(8): 385-395.
23.    Tiwari, K. and Gupta, R.K. 2012. Rare actinomycetes: a potential storehouse for novel antibiotics. Crit. Rev. Biotechnol.  32(2):108-32.
24.    Radhakrishnan M, Vijayalakshmi G, Gopikrishnan V and Jerrine J. Bioactive potential of actinobacteria isolated from certain understudied regions in India. J. Applied Pharma. Sci. 2016; 6(8): 151-155.
25.    Mohana S and Radhakrishnan M. Streptomyces sp MA7 isolated from mangrove rhizosphere sediment effective against Gram negative bacterial pathogens. International Journal of PharmTech Research. 2014; 6(4): 1259-1264.
26.    Diraviyam T, Radhakrishnan M and Balagurunathan R. Antioxidant activity of melanin pigment from Streptomyces species D5 isolated from Desert soil, Rajasthan, India Drug Invention Today. 2010; 3(3): 12-13.
27.    Knight V, Sanglier JJ, DiTullio D, Braccili S, Bonner P, Waters J, Hughes D and Zhang L. Diversifying microbial natural products for drug discovery. Appl. Microbiol. Biotechnol. 2003; 62: 446-458.
28.    Salamoni SP, Mann MB, Campos FS, Franco AC, Germani JC and Van der Sand ST. Preliminary characterization of some Streptomyces species isolated from a composting process and their antimicrobial potential. World J. Microbiol. Biotechnol. 2010; 26(10): 1847-1856.
29.    Lalitha C, Thiagarajan R, Sudarshan S, Rathore and Jayapradha R. 2010. Bioactive molecule from Streptomyces sp. mitigates MDR Klebsiella pneumoniae in Zebrafish infection model. Front. Microbiol. 2010; 8: 614.
30.    Treskatis SK, Orgeldinger V,  Wolf H and Gilles ED. Morphological characterization of filamentous microorganisms in submerged cultures by on-line digital image analysis and pattern recognition. Biotechnology and Bioengineering. 1997; 53(2):191-201.
31.    Manteca A, Alvarez R, Salazar N, Yague P and Sanchez J. Mycelium differentiation and antibiotic production in submerged cultures of Streptomyces coelicolor. Applied and Environmental Microbiology. 2008; 74(12): 3877-3886.

Recomonded Articles:

Author(s): Umesh Kumar, Rakesh Narang, Surendra Kumar Nayak, Sachin Kumar Singh, Vivek Gupta

DOI: 10.5958/0974-360X.2017.00425.5         Access: Open Access Read More

Author(s): Jino Elsa Thomas, Usha Y Nayak*, Jagadish PC, Koteshwara KB

DOI: 10.5958/0974-360X.2017.00007.5         Access: Open Access Read More

Author(s): Rakhi Mehra, Renu Makhija, Neera Vyas

DOI: Not Available         Access: Open Access Read More

Author(s): Koushika Das, Pranit Krishna, Avipsha Sarkar, Shanmuga Sundari Ilangovan, Shampa Sen

DOI: 10.5958/0974-360X.2017.00267.0         Access: Open Access Read More

Author(s): Raj Kumar R, Praveen D, Palani Shanmugasundaram

DOI: 10.5958/0974-360X.2016.00164.5         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): P. Geetha, M. Kousalya, S. Geetha Lakshmi, R. Gopi.

DOI: 10.5958/0974-360X.2016.00046.9         Access: Open Access Read More

Author(s): Siripurapu Sriram, Venkata Ravibabu Mandla, Chaithanya Sudha M, Saladi S.V. Subba Rao, Nagaveni Ch, Vani V., Gobinath P., Raj Kumar KVG., Jaydeep Lella, Sannidhi Krishna Praveen, Debjit Datta

DOI: 10.5958/0974-360X.2017.00078.6         Access: Open Access Read More

Author(s): Malarkodi Velraj, P. Jasmine Shiney, Biplab Paul, Rashmi. S, Nivethitha

DOI: 10.5958/0974-360X.2017.00006.3         Access: Open Access Read More

Author(s): Dhanalekshmi UnniKrishnan Nair, MP Saraswathy, Narra Kishore, Neelakanta Reddy Pully

DOI: Not Available         Access: Open Access Read More

Author(s): Narendra Chotai, Vishnu Patel, Harsha Patel, Uren Patel, Rajendra Kotadiya

DOI:         Access: Open Access Read More

Author(s): Barokar A.A., Wagh R.D., Baviskar D.T., Shaikh T.J.

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags