Development and Evaluation of Sodium ascorbyl phosphate Nanoemulsion for Transcutaneous Delivery

Fith Khaira Nursal; Yeyet C. Sumirtapura; Rahmana E. Kartasasmita; Hanifa Rahma; Tri Suciati

doi:10.5958/0974-360X.2020.00547.8

Research Journal of Pharmacy and Technology

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0974-360X (Online)
0974-3618 (Print)

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Development and Evaluation of Sodium ascorbyl phosphate Nanoemulsion for Transcutaneous Delivery

Author(s): Fith Khaira Nursal, Yeyet C. Sumirtapura, Rahmana E. Kartasasmita, Hanifa Rahma, Tri Suciati

Email(s): fithkhaira@uhamka.ac.id

DOI: 10.5958/0974-360X.2020.00547.8

Address: Fith Khaira Nursal1*, Yeyet C. Sumirtapura2, Rahmana E. Kartasasmita2, Hanifa Rahma2, Tri Suciati2
1Department of Pharmacy, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof DR. Hamka, Islamic Centre, Delima II/IV Street-Klender, East Jakarta 13470, Indonesia.
2School of Pharmacy, Institut Teknologi Bandung, Ganesha Street No.10-Bandung 40132, Indonesia.
*Corresponding Author

Published In: Volume - 13, Issue - 7, Year - 2020

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ABSTRACT:
Sodium ascorbyl phosphate (SAP) nanoemulsion has been developed using several methods with the aim of improving its skin permeation by transcutaneous delivery. SAP is a hydrophilic derivate of ascorbic acid with low permeability. Development of the formulation began with the utilization of glycerin as the solvent as well as co-surfactants, combined with the addition of amphiphilic molecules and lipophilic surfactants. Amphiphilic molecules and lipophilic surfactants formed complexes and emulsion, respectively, then underwent co-lyophilization prior to incorporating the lyophilizate to form nanoemulsion. Polyethylene glycol (PEG) 20000 and lecithin were used as the amphiphilic molecules, while Span 80 was employed as lipophilic surfactants. These methods aimed to improve the permeability of SAP, thus enhancing its permeation across the stratum corneum. The highest SAP solubility was achieved by adding 10% glycerin, which meant the glycerin could be used to increase the lipophilicity of SAP in the formulation. The amount of SAP partitioned into the oil phase was mixed by solid-in-oil dispersion (SOD) with PEG 20000, lecithin, and Span 80 was determined indirectly by measuring the concentration of SAP in the aqueous phase. The globule sizes of the SAP-PEG 20000 and SAP-lecithin nanoemulsions were 50–200nm and > 500nm for the SAP-Span 80 nanoemulsion. The amount of diffused SAP was varied in an in vitro permeation study using the Franz diffusion cell, snake skin (Python R.) and Spangler’s as a membrane models. This study indicates that SAP could penetrate in to the skin by SOD method and its potential utility to improve the skin permeation of hydrophilic molecules.

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Cite this article:
Fith Khaira Nursal, Yeyet C. Sumirtapura, Rahmana E. Kartasasmita, Hanifa Rahma, Tri Suciati. Development and Evaluation of Sodium ascorbyl phosphate Nanoemulsion for Transcutaneous Delivery. Research J. Pharm. and Tech. 2020; 13(7): 3086-3092. doi: 10.5958/0974-360X.2020.00547.8

Cite(Electronic):
Fith Khaira Nursal, Yeyet C. Sumirtapura, Rahmana E. Kartasasmita, Hanifa Rahma, Tri Suciati. Development and Evaluation of Sodium ascorbyl phosphate Nanoemulsion for Transcutaneous Delivery. Research J. Pharm. and Tech. 2020; 13(7): 3086-3092. doi: 10.5958/0974-360X.2020.00547.8 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-7-7

REFERENCES:

1. Tahara, Y., Namatsu, K., Kamiya, N., Hagimori, M., Kamiya, S., Arakawa, M., Goto. M. Transcutaneous immunization by a solid-in-oil nanodispersion. Chem. Commun. 2010; 46; 9200-9202.

2. Tahara, Y., Kamiya, N., Goto, M. Solid-in-oil dispersion: a novel core technology for drug delivery systems. Int J Pharm. 2012; 438: 249-257.

3. Hirakawa, Y., Wakabayashi, R., Naritomi, A., Sakuragi, M., Kamiya, N., Goto, M. Transcutaneous immunization againts cancer using a solid-in-oil nanodispersion. Med. Chem. Comm.. 2015; 6: 1387-1392.

4. Suciati, T., Aliyandi, A., Satrialdi. Development of transdermal nanoemulsion for simultaneous delivery of protein vaccine and artin-M adjuvant. Int. J. Pharm Pharm. Sci. 2014;6(6): 536-546.

5. Suciati, T., Samhana, A., Mauludin, R. Development of jacalin conyugated nanostuctured lipid carriers formula for transcutaneous vaccine delivery. Int. J. Pharm. Pharm. Sci. 2014; 6(6): 542-547.

6. BASF, Germany. Technical Information Sodium Ascorbyl Phosphate. 2005.

7. Austria R., Semenzato, A., Bettero. A stability of vitamin C derivatives in solution and topical formulations. J Pharm Biomed Anal.1997;15: 795–801.

8. Jadhav. K.R., Kadam V.J.S.P. Sambhaji. Stability enhancement and in-vitro skin permeation of erythromycin from lecithin-IPM organogel. Research J. Pharm. and Tech. 2008; 1(1): 33-39.

9. Sankari K.U., M. Alagusundaram, Sahitihi G.K., Chetty. C.M.S., Ramketh S., Angalaparameswari S., Mohammed Salem TS. Nanoemulsions: approaching thermodynamic stability. Research J. Pharm. and Tech. 2010; 3(2): 319-326.

10. Tahara, Y., Kamiya, N., Goto, M. Transdermal delivery of insulin using a solid-in-oil nanodispersion enhanced by arginine-rich peptides. Med Chem Comm. 2012; 3: 1496-1499.

11. Morita, T., Sakamura, Y., Horokiri, Y., Suzuki, T., Yoshino, H. Protein encapsulation into biodegradable microsphere by a novel S/O/W emulsion method using poly (ethylene glycol) as a protein micronization adjuvant. J Control Release. 2000; 69:435-444.

12. Piao, H., Kamiya, N., Cui, F., Goto, M. Preparation of a solid-in-oil nanosuspension containing L-ascorbic acid as a novel long-term stable topical formulation. Int J Pharm. 2011; 420:156-160.

13. Tahara, Y., Honda, S., Kamiya, N., Piao, H., Hirata, A., Hayakawa, E., Fujii, T., Goto, M. A solid-in-oil nanodispersion for transcutaneous protein delivery.J Control Release.2008; 131:14-18.

14. Piao, H., Kamiya, N., Hirata, A., Fujii, T., Goto, M. A novel solid-in-oil nanosuspension for transdermal delivery of diclofenac sodium. Pharm Res. 2008; 25(4): 896-901.

15. Spiclin, C., Homar, M., Valan, A.Z., Gasperlin, M. Sodium ascorbyl phosphate in topical microemulsions. Int J Pharm. 2003; 256: 65–73.

16. Segall, A.I and Moyano, M. A. Stability of vitamin C derivatives in topical formulations containing lipoc acid, vitamin A and E. Int J Cosmet Sci. 2008; 30: 453-458.

17. Kong, M., Park, H.J. Investigations on skin permeation of hyaluronic acid based nanoemulsion as transdermal carrier. Carbohydr Polym.2011; 86(2): 837-843.

18. Krishna, G.A.G., Raj., G., Bhatnagar, A.S., P.K., Kumar, P., Chandrashekar, P. Coconut oil: Chemistry, production and its application-a review. Indian coconut Journal. 2010; 15-27.

19. Morita, Y., Horikiri, Y., Yamahara, H., Suzuki, T., Yoshino, H. Formation and isolation of spherical fine protein microparticles through lyiophilization of protein-poly (ethylene glycol) aqueous mixture. Pharm Res.2000; 17 (11): 1367-1373.

20. Klang, C., Valenta, C. Lecithin-based nanoemulsion. J. Drug Deliv Sci. Technol. 2011; 21 (1): 55-76.

21. Zhou, H., Yue, Y., Liu, G., Li, Y., Zhang, J., Gong, Q., Yan, Z., Duan, M. Preparation and characterization of a lecithin nanoemulsion as a topical delivery system. Nanoscale Res Lett (Nano express). 2010; 5:224-230.

22. Taylor, P. Ostwald ripening in emulsion. Colloid and Surface A: Physicochemical and Engineering Aspect. 1995; 99:175-185.

23. Baschong. Ciba® Speciality Chemical. 2005; Switzerland.

24. Ngawhirunpat, T., Panomsuk, S., Opanasopit, P., Rojanarata, T., Hatanaka, T. Comparison of the percutaneous absorption of hydrophilic and lipophilic compounds in shed snake skin and human skin. Pharmazie. 2006;61: 331-335.

25. Itoh, T., Xia, J., Magavi, R., Nishihata, T., Rytting, J.H. Use of sheed snake skin as model membrane for in vitro percutaneous penetration studies: comparison with human skin. Pharm Res. 1990; 7(10); 1042-1047.

26. Spangler, W.G., Cross, H.D., Schaafsma, B.R. A laboratory method for testing laundry product for detergency. J Am Oil Chem Soc. 1965; 42: 723-727.

27. Ali S., Shabbir M., Shahid N. The structure of skin and transdermal dru delivery system- a review. Research J. Pharm. And Tech. 2015; 8(2): 103-109.

28. Ali S., Shabbir M., Farooq M., Adnan S., Shaihd N. The enhancement effect of permeation enhancers on bisoprolol fumarate across animal membrane using Franz diffusion cell. Research J. Pharm. And Tech. 2014; 7(12): 1391-1395.