Author(s): Preeti Kalia, Neelima R. Kumar, Kusum Harjai

Email(s): preeti.kalia84@gmail.com

DOI: 10.5958/0974-360X.2020.00602.2   

Address: Preeti Kalia1*, Neelima R. Kumar2, Kusum Harjai3
1Department of Zoology, GGDSD College, Chandigarh, India. 2Department of Zoology, Panjab University, Chandigarh, India.
3Department of Microbiology, Panjab University, Chandigarh, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 7,     Year - 2020


ABSTRACT:
Apitherapy is a branch of alternative medicine which deals with the therapeutic uses of all the honey bee or bee hive products. The present research aimed to study the effect of pretreated groups of experimental mice with one of the potent honey bee product i.e propolis and then infecting the animals with Typhoid causing bacteria i.e. Salmonella. Survival percentage, bacterial load and hematological parameters were observed. The bacterial load in blood and spleen was significantly reduced in case of propolis pretreated groups of mice. Moreover, the RBC count and WBC count in one of pretreated group was 8.4±0.52 million/mm3 and 7366.66±160.72/mm3 respectively which was significantly higher than that of infected control. So, from the results it was clearly evident that pretreatment with propolis decreases the severity of infection in mice.


Cite this article:
Preeti Kalia, Neelima R. Kumar, Kusum Harjai. Preventive effect of Honey bee propolis on Salmonella enterica serovar Typhimurium infected BALB/c mice: A Hematological Study. Research J. Pharm. and Tech. 2020; 13(7): 3389-3393. doi: 10.5958/0974-360X.2020.00602.2


REFERENCES:
1. Ratcliffea N A, Melloc C B, Garciaa E S, Buttb T M and Azambujaa P. Insect natural products and process: New treatment for human diseases. Insect Biochemistry and Molecular Biology, 2011; 41(10): 747-69.
2. Drago L, de Vecchi E, Nicola L and Gismondo M. In vitro antimicrobial activity of a novel propolis formulation actichelated propolis. Journal of Applied Microbiology, 2007; 103: 1914-1921. 
3. Kalia P, Kumar N R and Harjai K. The therapeutic potential of propolis against the damage caused by Salmonella typhimurium in liver of mice: a biochemical and histological study. Archives of Biological Sciences,2015;67(3):807-16. doi:0354-46641500040K.
4. Al- Ani I, Zimmermann S, Reichling J and Wink M. Antimicrobial activities of European propolis collected from various geographic origins alone and in combination with antibiotics. Medicines (Basel), 2018; 5(1): 2.
5. Grimont P A D and Weill F X. Antigenic formulae of the Salmonella serovars, 9th Edn. World Health Organization collaborating centre for reference and research on Salmonella. Institute Pasteur, Paris, France.2007.
6. Chandel D S, Chaudhry R, Dhawan B, Pandey A and Dey A B. Drug-resistant Salmonella enterica serotype Paratyphi A in India. Emerging Infectious Diseases, 2000; 6: 420-421.
7. Gasem M H, Dolmans W M, Keuter M M and Djokomoeljanto R R. Poor food hygiene and housing as risk factors for typhoid fever in Semarang, Indonesia. Tropical Medicine and International Health, 2001;6: 484-90.
8. Chidozie V N and Adoga G I. Potentiating effect of aqueous leaf extract of Anogeissus leiocarpuson Carica papaya aqueous leaf extract and Mangifera indica aqueous stem bark extract - A herbal product used against typhoid fever in Nigeria. International Journal of Current Microbiology and Applied Sciences, 2014; 3(10): 1046-1062.
9. Gillespie, S. H. (2003). Salmonella infections. In: Manson’s tropical diseases. 21st Edn. (G. C. Cook and A. Zumla, eds.) W. B. Saunders/Elsevier, London publs. pp. 937-49.
10. Crump J A, Luby S P and Mintz E D. The global burden of typhoid fever. Bulletin of the World Health Organization, 2004;82: 346-353.
11. WHO, “Food Safety-Food borne diseases and value chain management for food safety,” in Proceedings of the Forging Links between Agriculture and Health, CGIAR on Agriculture and Health Meeting, 2007.
12. Crump J and Mintz E. Global trends in typhoid and paratyphoid Fever. Clinical Infectious Diseases, 2010;50: 241-246.
13. Clark T W, Daneshvar C, Pareek M, Perera N and Stephenson I. Enteric fever in a UK regional infectious diseases unit: A 10 year retrospective review. Journal of Infection, 2010; 60(2): 91-98.
14. Eng S K, Pusparajah P, Ab- Mutalib N S, Ser H, Chand K G and Lee L H. Salmonella: A review on pathogenesis, epidemiology and antibiotic resistance. Frontiers in Life Science, 2015; 8(3): 284–293.
15. Klemm E J, Shakoor S, Page A J, Qamar F N, Judge K, Saeed D K, Wong V K. et al. Emergence of an extensively drug-resistant Salmonella enterica Serovar Typhi clone harboring a promiscuous plasmid encoding resistance to fluoroquinolones and third-generation cephalosporins. mBIO, 2018;doi:10.1128/mBio.00105-18.
16. Adeneya A A, Ajagbonna O P, Adeleke T I and Bello S O. Preliminary toxicity and phytochemical studies of the stem bark aqueous extract of Musanga cecropioides in rats. Journal of Ethnopharmacology, 2006; 105: 374-379.
17. Promise N, Agomuo E N, Uloneme G C, Egwurugwu J N, Omeh Y N and Nwakwuo G C. Effect of Phyllanthus amarus leaf extract on alterations of haematological parameters in Salmonella typhi infected wistar albino rats. Scientific Research and Essays, 2014; 9(1): 7-12.
18. Kalia P, Kumar N R and Harjai K. Phytochemical screening and antibacterial activity of different extracts of propolis. International Journal of Pharmaceutical and Biological Research, 2013; 3(6): 219-22.
19. Kakkar K, Sharma S, Asnani P J, Banerjee C K and Sharma B K. Experimental haematogenous pyelonephritis in mice with uropathogenic, enteropathogenic and enterotoxigenic Escherichia coli. Antonie Van Leewenhoek, 1986;52(2): 153-61.
20. Jain N C. Hematologic technique. In: Schalm’s Veterinary hematology. 4th Edn. (Lea and Febiger eds.) Philadelphia publs. pp. 20-86. 1986.
21. Kalia P, Kumar N R and Harjai K. Effect of propolis extract on the haematotoxi-city and histological changes induced by Salmonella enterica serovar Typhimurium in BALB/c mice. Archives of Biological Sciences, 2016;68(2):385-91 doi:10.2298/AB-S150902030K.
22. Makela P H and Hormaeche C E. Immunity to Salmonella. In: Host response to intracellular pathogens, (S. H. E. Kaufmann, ed.) Austin and Landes publs. pp. 143-66. 1997.
23. Zghair Z R. Histopathological study of Salmonella typhimurium infection in laboratory mice by using the light and electron microscope. Kufa. Journal of Veterinary Medical Sciences, 2012; 3(1): 124-31.
24. Boyanova L, Gergova G, Nikolov R, Derejian S, Lazarova E and Katsarov N. Activity of Bulgarian propolis against 94 Helicobacter pyroli strains in vitro by agar well diffusion, agar dilution and disc diffusion methods. Journal of Medical Microbiology, 2005; 54(5): 481-483.
25. Abro A H, Abdou A M, Gangwani J L, Ustadi A M, Younis N J and Hussaini H S. Hematological and biochemical changes in typhoid fever. Pakistan Journal of Medical Sciences, 2009; 25(2): 166-171.
26. Dangana A, Ajobiewe J and Nuhu A. Haematological changes associated with Salmonella typhi and Salmonella paratyphi in humans. International Journal of Biomedical and Healthcare Science, 2010; 6: 219-222.
27. Kumar K B H and Kuttan R. Chemopreventive activity of an extract of Phyllanthus amarus against cyclophosphamide induced toxicity in mice. Phytomedicine, 2005; 7: 494-500.
28. Lokhandwala A, Athar S and Turrin N P. Role of absolute eosinopenia as marker of enteric fever: experience from a tertiary care hospital in the United Arab Emirates. Ibnosina. Journal of Medical and Biomedical Sciences, 2012; 4(6): 249-53.
29. Haro A, Lopez-Aliaga I, Lisbona F, Barrionueva M, Alferez M J and Campos M S. Benefical effect of pollen and/or propolis on the metabolism of iron, calcium, phosphorus and magnesium in rats with nutritional ferropenic anemia. Journal of Agricultural and Food Chemistry, 2000; 48: 5715-22.
30. Youdim K A, Shukitt-Hale B, MacKinnon S, Kalt W and Joseph J A. Polyphenolics enhance red blood cell resistance to oxidative stress: In vitro and in vivo. Biochimica Et Biophysica Acta, 2000; 1523: 117-22.
31. Cushnie T T P and Lamb A J. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 2005; 26: 343-356.
32. Kaur R, Kumar N R and Harjai K. Phytochemical analysis of different extracts of bee pollen. International Journal of Pharmaceutical and Biological Research, 2013; 4(3): 65-68.
33.  Manore D, Pillai S, Joshi A and Punashiya R. Preliminary Phytochemical Screening and Antibacterial Activity of Ethyl Acetate Extract of Cuscuta reflexa Roxb. Research Journal of Pharmacy and Technology, 2012; 5(1).

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