Alangium salvifolium, commonly known as Ankol tree. In Ayurveda, the roots and the fruits are used for the treatment of hemorrhoid and rheumatism. Traditionally the seeds Alangium salvifolium exhibit various activities including antimicrobial, anticancer, Antidiabetic, diuretic, anti-inflammatory, laxative, and antiepileptic activity. The main objective of the present work is to establish the pharmacological potential of Alangium salvifolium seeds in terms of its phytochemical composition and anti-oxidant potential. In order to fulfill the objectives of proposed work, three different types of extracts viz, aqueous, hydro-alcoholic and alcoholic extracts of Alangium salvifolium seeds were prepared using Soxhlet extraction technique and cold maceration technique respectively. The extracts were subjected to qualitative phytochemical analysis. The TLC was performed for the three extracts and the alcoholic extract was further carried out for preparative TLC and fractions were separated as B1, B2, B3, B4 and B5 which shows the presence of alkaloids, glycosides and steroids. All the extracts and fractions were subjected to anti-oxidant activity by three different assays that are DPPH scavenging activity, Hydrogen peroxide activity and Reducing capacity assay and % inhibition was observed in all three extracts and five fractions of alcoholic. The present study provides qualitative phytochemical analysis and anti-oxidant potential of Alangium salvifolium seeds.
Cite this article:
Sachin Bhusari, Shreya Waghmare, Kanchan Nikam, Pravin Wakte. In-Vitro Anti-Oxidant Activity and Free Radical scavenging Potential of Alangium salvifolium seeds. Research J. Pharm. and Tech. 2020; 13(7): 3081-3085. doi: 10.5958/0974-360X.2020.00546.6
Sachin Bhusari, Shreya Waghmare, Kanchan Nikam, Pravin Wakte. In-Vitro Anti-Oxidant Activity and Free Radical scavenging Potential of Alangium salvifolium seeds. Research J. Pharm. and Tech. 2020; 13(7): 3081-3085. doi: 10.5958/0974-360X.2020.00546.6 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-7-6
1. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry and Cell Biology 2007; 39 (1): 44–84.
2. Valko M, Izakovic M, Mazur M, Rhodes CJ, Telser J. Role of oxygen radicals in DNA damage and cancer incidence. Molecular and Cellular Biochemistry 2004; 266 (1–2): 37–56.
3. Ravirala V, Akondi BR, Yerragunta VG. Phytochemistry and pharmacology of Alangium salvifolium: A review. Journal of Pharmacy Research 2011; 4(5).
4. Ratra M, Gupta R. Comprehensive Review on Pharmacological Profile of Alangium salvifolium: A Medicinal Plant. UK Journal of Pharmaceutical and Biosciences 2015; 3(3): 22-28.
5. Sharma AK, Agrawal V, Kumar R. Pharmacological studies on seeds of Alangium salvifolium Linn. Acta Poloniae Pharmaceutica n Drug Research; Polish Pharmaceutical Society.2011; 68 (6): 897-904.
6. Ahad HA, Suma PB. Phytochemical screening and anti-inflammatory actions of Alangium salvifolium root extract. Natural Product Research 2012; 26(17): 1649–1653.
7. Ravichandran S, Balamurugan N, Barathi SP. Phytochemical analysis of Alangium salvifolium (Linn) leaf extracts. Asian Journal of Phytomedicine and Clinical Research 2015; 3(2): 46 – 49.
8. Kalarani DH, Venkatesh P. Isolation of Phytochemical Constituent, Characterization and Pharmacognostic Studies of Stem and Leaves of Alangium salvifolium Wang. The Pharmaceutical and Chemical Journal 2017; 4(5): 89-97.
9. Jain VC, Patel NM, Shah DP. Antioxidant and Antimicrobial Activities of Alangium salvifolium (L.F) Wang Root. Global Journal of Pharmacology 2010; 4 (1): 13-18.
10. Tanwer BS and Vijayvergia R. Biological evaluation of Alangium salvifolium (L.F.) Wangerin. Journal of Chemical and Pharmaceutical Research 2014; 6(12): 611-618.
11. Kumar R, Pate DK, Prasad SK, Krishnamurthy S, Siva H. Antidiabetic activity of alcoholic leaves extract of Alangium lamarckii Thwaites on streptozotocin-nicotinamide induced type 2 diabetic rats. Asian Pacific Journal of Tropical Medicine 2011; 904-909.
12. Sreekanth P, Sudhakara K, Gousebasha G, Murali K, Kumar S. Anti-ulcer effect of Alangium salvifolium ethanolic leaf extract on gastric lesion induced by ethanol in rats. Asian journal of Pharmaceutical and Clinical Research 2011; 4(2): 112-114.
13. Sakthidevi G, Mohan VR, Jeeva S. In vitro antioxidant activity of leaf extracts of Alangium salvifolium (L.f.) Wang (Alanginaceae). Bioscience Discovery 2014; 5(1): 74-81.
14. Jain VC, Patel NM, Shah DP, Patel PK and Joshi BH. Antioxidant and Antimicrobial Activities of Alangium salvifolium (L.F) Wang Root. Global Journal of Pharmacology 2010; 4(1): 13-18.
15. Edamatsu R, Mori A, Fujita Y, Yasuhara E. Chem. pharma. Bull 1989; 37: 2016 – 2027.
16. Crates DP. Free radicals and aging. Ann Biol Clin 1990; 48: 323.
17. Seifried HE, Anderson DE, Fisher EI, Milner JA. A review of the interaction among dietary antioxidants and reactive oxygen species. J Nutr Biochem 2007; 18: 567-579.
18. Shindo Y, Witt E, Packer L. Antioxidant defense mechanisms in murine epidermis and dermis and their response to ultraviolet light. J Invest Dermatol 1993; 100: 260-5.