Author(s): S. Karthika, N. Kannappan, TNK Suriyaprakash


DOI: 10.5958/0974-360X.2020.00595.8   

Address: S. Karthika1, N. Kannappan2, TNK Suriyaprakash1
1Department of Pharmaceutical Chemistry, Al Shifa College of Pharmacy, Ponthavannam P.O, Perinthalmanna, Kerala-679325.
2Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu-608002.
*Corresponding Author

Published In:   Volume - 13,      Issue - 7,     Year - 2020

Amyloid-beta peptide (Aß) is implicated in the pathogenesis of Alzheimer’s disease (AD), a neurodegenerative disorder. The present study was designed to determine the effect of two medicinal plants used to treat neurodegenerative diseases on Amyloid beta intoxicated SHSY5Y cell line. Cytotoxicity of the different extracts of the two medicinal plants was determined against SH-SY5Y (human neuroblastoma) cells which were untreated, as well as toxically induced with Aß, using the MTT assay. The n-Hexane extract of Hydrocotyle javanica (HJ) and Alcoholic extract of Peristrophe bicalyculata (PB) were the least toxic to the SH-SY5Ycells at the highest concentration tested (250µg/ml). All different extracts of two medicinal plants tested were observed to reduce the effects of Aß-induced neuronal cell death, indicating that they may contain compounds which may be relevant in the prevention of AD progression.

Cite this article:
S. Karthika, N. Kannappan, TNK Suriyaprakash. Effect of Medicinal plants on amyloid β1-42 Intoxicated SH-SY5Y cell Lines - As Neuroprotective Evaluation. Research J. Pharm. and Tech. 2020; 13(7): 3351-3355. doi: 10.5958/0974-360X.2020.00595.8

S. Karthika, N. Kannappan, TNK Suriyaprakash. Effect of Medicinal plants on amyloid β1-42 Intoxicated SH-SY5Y cell Lines - As Neuroprotective Evaluation. Research J. Pharm. and Tech. 2020; 13(7): 3351-3355. doi: 10.5958/0974-360X.2020.00595.8   Available on:

1. Adewusi EA, Moodley N, Steenkamp V Medicinal plants with cholinesterase inhibitory activity: A Review. Afr. J. Biotechnol. 2010; 9; 8257-8276.
2. Chiba T, Nishimoto I, Aiso S, Matsuoka M. Neuroprotection against neurodegenerative diseases. Development of a novel hybrid neuroprotective peptide colivelin. Mol. Neurobiol. 2007; 35; 55-84.
3. Silva, B.A., Dias, A.C.P., Ferreres, F., Malva, J.O., and Oliveira, C.R. Neuroprotective effect of H. perforatum extracts on β-amyloid induced neurotoxicity. Neurotox. Res. 2004; 6; 119-130.
4. Coyle, J., and Kershaw, P. Galantamine, a cholinesterase inhibitor that allosterically modulates nicotinic receptors: effects on the course of Alzheimer’s disease. Biol. Psychiatry 2001; 49; 289-299.
5. Pereira, C., Agostinho, P., Moreira, P.I., Cardoso, S.M., and Oliveira, C.R. Alzheimer’s disease-associated neurotoxic mechanisms and neuroprotective strategies. Curr. Drug Targets: CNS and Neurol. Disord. 2005; 4: 383-403.
6. Ji, Z.N., Dong, T.T.X., Ye, W.C., Choi, R.C., Lo, C.K., and Tsim, K.W.K. Ginsenoside Re attenuate β-amyloid and serum-free induced neurotoxicity in PC12 cells. J. Ethnopharmacol. 2006; 107: 48-52.
7. Findeis, M.A. Approaches to discovery and characterization of inhibitors of amyloid-beta peptide polymerization. Biochim. Biophys. Acta . 2000; 1502; 76-84.
8. Kawahara, M., and Kuroda, Y. Molecular mechanism of neurodegeneration induced by Alzheimer’s beta-amyloid protein: channel formation and disruption of calcium homeostasis. Brain Res. Bull. 2000; 53; 389-397
9. Puttfarcken, P.S., Manelli, A.M., Neilly, J., and Frail, D.E. Inhibition of age-induced beta-amyloid neurotoxicity in rat hippocampal cells. Exp. Neurol. 1996; 138; 73-81.
10. Boyd-Kimball, D., Mohammad Abdul, H., Reed, T., Sultana, R., and Butterfield, D.A. Role of phenylalanine 20 in Alzheimer’s amyloid beta-peptide (1-42)-induced oxidative stress and neurotoxicity. Chem. Res. Toxicol. 2004; 17; 1743-1749.
11. Martin, S.E., de Fiebre, N.E., and de Fiebre, C.M. The alpha7 nicotinic acetylcholine receptor-selective antagonist, methylcaconitine, partially protects against beta-amyloid1-42 toxicity in primary neuron-enriched cultures. Brain Res. 2004; 1022; 254256.
12. Limpeanchob, N., Jaipan, S., Rattanakaruna, S., Phrompittayarat, W., and Ingkaninan, K. Neuroprotective effects of Bacopa monnieri on beta-amyloid-induced cell death in primary cortical culture. J. Ethnopharmacol. 2008; 120; 112-117.
13. Kang, I-J., Jeon, Y.E., Yin, X.F., Nam, J-S., You, S.G., Hong, M.S., Jang, B.G., and Kim, M-J. Butanol extract of Ecklonia cava prevents production and aggregation of beta-amyloid and reduces beta-amyloid mediated neuronal death. Food Chem. Toxicol. 2011; 49; 2252-2259.
14. Yu, M-S., Leung, S.K-Y., Lai, S-W., Che, C-M., Zee, S-Y., So, K-F., Yuen, W-H., and Chang, R.C-C. Neuroprotective effects of anti-aging oriental medicine Lyciumbarbarum against β-amyloid peptide neurotoxicity. Exp. Gerontol. 2005; 40; 716-727.
15. Pattewar AV, Katedeshmukh RG, Vyawahare NS, Kagathara VG. Phytomedicines and Cognition. Int. J. Pharm. Sci. Res. 2011; 2(4); 778-91.
16. Beverly CD, Sudarsanam G (2011). Ethnomedicinal plant knowledge and practice of people of Javadhu hills in Tamilnadu. Asian Pac. J. Trop. Biomed. 1(1):79-81.
17. Dike IP, Obembe OO, Adebiyi FE (2012). Ethnobotanical survey for potential anti-malarial plants in south western Nigeria. J. Ethnopharmacol. 144(3):618-26.
18. World Health Organization (WHO). Legal status of traditional medicine and complementary/alternative medicine: A worldwide review. 2001
19. WHO Publishing World Health Organization (WHO). Traditional Medicine Growing Needs and Potential-WHO Policy Perspectives on Medicines, 2002;002. Available at: medicine docs/ en/d/ Js2293e
20. Elujoba AA, Odeleye OM, Ogunyemi CM. Traditional medical development for medical and dental primary health care delivery system in Africa. Afr. J. Tradit. Complement. Altern. Med. 2005;2(1); 46-61
21. A.K. Nadkarni, D.K.M. Nadkarni (rd ed.), Nadkarni's Indian Materia Medica: with ayurvedic, unani-tibbi, siddha, allopathic, homeopathic, naturopathic and home remedies, Popular Prakashan, Bombay. 1976; 3;1,2.
22. S.R. Downie, K. Spalik, D.S. Katz-Downie, J.P. Reduron Major clades within Apiaceae subfamily Apioideae as inferred by Fyson PY. 1932.
23. P.Y. Fyson (Ed.), The flora of South Indian hill station, Govt. Press, Madras 2010.
24. A.G. Prasad, T.B. Shyma Medicinal plants used by the tribes of Vythiri Taluk, Wayanad district (Kerala state) for the treatment of human and domestic animal ailments J Med Plants Res. 2013; 7 (20); 1439-1451
25. M. Mandal, S. Paul, M.R. Uddin, M.A. Mandal, S. Mandal, V. MandalIn vitro antibacterial potential of Hydrocotyle javanica Thunb. Asia Pac J Trop Dis, 2016; 6 (1); 54-62
26. Annonyms, “Wealth of India”, Raw Materials, Publication and Information Directorate, CSIR, New Delhi, 1966; 8; 313.
27. Nadkarni. K.M., “Indian Material Medica”. Bombay Popular Prakashan, 1982; 1; 933.
28. Chopra R.N., Nayar S.L., Chopra I.C. Glossary of Indian Medicinal Plants. National Institute of Science Communication Publication, New Delhi, 1996; 189.
29. Chopra R. N., Chopra I.C., Varma B.S., Supplement to Glossary of Indian Medicinal Plants, Publications and Information Directorate, New Delhi, 1974; 79.
30. Agarwal V.S. Drug Plants of India. Kalyani Publishers, Ludhiana, 1997;2(1); 549.
31. Singh V.K., Govil J.N., Hashmi Shamima, Singh Gurdip. Recent Progress in Medicinal Plants. Ethnomedicine and Pharmacognosy II, Vol. 7, 2007; 7; 90-134.
32. Available at: http://www. publish/ journal/_1049.txt Accessed 2008.
33. ALUKA resources page. “Entry for Peristrophebicalyculata (Retz) Nees (Family Acantahaceae)” Accessed – January 11, 2008.
34. Available at: http://
35. Singh R.S., Pandey R.P., Singh B.K. and Singh R.G.: Aliphatics from Peristrophebicalyculata. Fitoterapia, 2000; 71(1); 80-81.
36. Carried through Medicinal and Aromatic Plant Abstract.2000; 5.
37. Dr. Pulok K. Mukherjee, Quality Control of Herbal drugs. Published by Business Horizons, 2002; 1; 303
38. Indian Herbal Pharmacopoeia. Indian Drug Manufacture Association. 1999; 2; 174 – 175
39. Ashutoshkar, Pharmacognosy and Pharmacobiotechnology. New Age International Publishers, 2007; 2; 945
40. Pulok. K. Mukherjee. Quality Control of Herbal Drugs. Business Horizons, New Delhi, 2002; 1; 284
41. W.C. Evans. Trease and Evans, Pharmacognosy. Elsevier. 2005; 15; 193.
42. C.K. Kokate, A.P. Purohit, S.B. Gokhale, Pharmacognosy. Nirali Prakashan, Pune, 2005; 33; 108-109.
43. Masahiko Kurokawa, Ashish Wadhwani, Hisahiro Kai, Muneaki Hidaka, Hiroki Yoshida, Chihiro Sugita, Wataru Watanabe, Koji Matsuno, and Akinori Hagiwara. Activation of Cellular Immunity in Herpes Simplex Virus Type 1- Infected Mice by the Oral Administration of Aqueous Extract of Moringa oleifera Lam. Leaves. Phytotherapy Research, 2016; 30(5): 797–804

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