Molecular Identification of Trichoderma Isolates from Sugarcane Bagasse Based on Internal Transcribed Spacer (ITS) rDNA

Siti Rukmana; Arif N. M. Ansori; Muhammad K. J. Kusala; Ulfah Utami; Didik Wahyudi; Andita A. Mandasari

doi:10.5958/0974-360X.2020.00585.5

Research Journal of Pharmacy and Technology

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0974-360X (Online)
0974-3618 (Print)

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Molecular Identification of Trichoderma Isolates from Sugarcane Bagasse Based on Internal Transcribed Spacer (ITS) rDNA

Author(s): Siti Rukmana, Arif N. M. Ansori, Muhammad K. J. Kusala, Ulfah Utami, Didik Wahyudi, Andita A. Mandasari

Email(s): andita_ayu_mandasari@dosen.umaha.ac.id

DOI: 10.5958/0974-360X.2020.00585.5

Address: Siti Rukmana1, Arif N. M. Ansori1,2, Muhammad K. J. Kusala1, Ulfah Utami3, Didik Wahyudi3, Andita A. Mandasari4*
1Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Computational Virology and Complexity Science Research Unit, Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia.
3Faculty of Science and Technology, UIN Maliki Malang, Malang, Indonesia.
4Faculty of Health Science, Universitas Maarif Hasyim Latif, Sidoarjo, Indonesia.
*Corresponding Author

Published In: Volume - 13, Issue - 7, Year - 2020

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ABSTRACT:
This study aimed to identify Trichoderma from sugarcane bagasse based on iinternal transcribed spacer (ITS) rDNA. The sample used was pure isolate of Trichoderma from sugarcane bagasse. DNA samples were isolated using the modified CTAB method. DNA was amplified using the primers ITS1 and ITS4. Interestingly, the results showed that Trichoderma genomic DNA has a concentration of 92.56 mg with a purity of 1.91. The amplicon of Trichoderma DNA is about 600 bp, whereas phylogenetic analysis shows that the sample of Trichoderma from sugarcane bagasse is one group with Trichoderma harzianum, Trichoderma piluliferum, Trichoderma sp. SQR339, Hypocrea nigricans, and Trichoderma sp. NFML CH12 BB. 15, Trichoderma aureoviride, Hypocrea lixii, and Trichoderma BAB-4585.

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Cite this article:
Siti Rukmana, Arif N. M. Ansori, Muhammad K. J. Kusala, Ulfah Utami, Didik Wahyudi, Andita A. Mandasari. Molecular Identification of Trichoderma Isolates from Sugarcane Bagasse Based on Internal Transcribed Spacer (ITS) rDNA. Research J. Pharm. and Tech. 2020; 13(7): 3300-3304. doi: 10.5958/0974-360X.2020.00585.5

Cite(Electronic):
Siti Rukmana, Arif N. M. Ansori, Muhammad K. J. Kusala, Ulfah Utami, Didik Wahyudi, Andita A. Mandasari. Molecular Identification of Trichoderma Isolates from Sugarcane Bagasse Based on Internal Transcribed Spacer (ITS) rDNA. Research J. Pharm. and Tech. 2020; 13(7): 3300-3304. doi: 10.5958/0974-360X.2020.00585.5 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-7-45

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