Doxorubicin (DOX) is widely used in the treatment of various human neoplasms. The development of severe undesirable effects such as cardiotoxicity, myelotoxicity, GI Toxicity limits its therapeutic efficacy. The present study explores the beneficial effect of alcoholic extract of roots of Lawsonia inermis (LIALC) against doxorubicin induced cardiotoxicity and also to assess the antitumor efficacy of the combination treatment on tumor bearing mice. Cardiotoxicity was evaluated by measuring serum biomarkers glutamic oxalo transaminase (GOT), glutamic Pyruvic transaminase (GPT), alkaline phosphatase (ALP), creatinine and urea and cardiac antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and lipid peroxidation (LP) in heart homogenate. After treatment with extract for 2 weeks in doxorubicin intoxicated mice, the antitumor activity of combined treatment was determined by mean survival time. Toxicity was evidenced by increased levels of serum biomarkers and decreased levels of cardiac antioxidant enzymes. Treatment with extract provided substantial protection by altering the above parameters to near normal level and prolonging the survival time of tumorous mice. However these results suggest that extract of roots of Lawsonia inermis has the ability to reduce cardiotoxicity and ameliorate doxorubicin antitumor effect.
Cite this article:
Divya B, Shivashree S, K. Mruthunjaya, S. N. Manjula. Cardioprotective activity of Lawsonia inermis roots against Doxorubicin Treated Mice. Research J. Pharm. and Tech. 2020; 13(7): 3279-3283. doi: 10.5958/0974-360X.2020.00581.8
1. Arcamone F. Properties of Antitumor Anthracyclines and New Developments in Their Application: Cain Memorial Award Lecture. Cancer research. 1986 Jan 1; 45:5995–9.
2. Blum RH, Carter SK. Adriamycin. A new anticancer drug with significant clinical activity. Ann Intern Med. 1974 Feb;80(2):249–59.
3. Volkova M, Russell R. Anthracycline Cardiotoxicity: Prevalence, Pathogenesis and Treatment. Curr Cardiol Rev. 2011 Nov;7(4):214–20.
4. Kantrowitz NE, Bristow MR. Cardiotoxicity of antitumor agents. Prog Cardiovasc Dis. 1984 Dec;27(3):195–200.
5. McGowan JV, Chung R, Maulik A, Piotrowska I, Walker JM, Yellon DM. Anthracycline Chemotherapy and Cardiotoxicity. Cardiovasc Drugs Ther. 2017;31(1):63–75.
6. Wiseman LR, Spencer CM. Dexrazoxane. A review of its use as a cardioprotective agent in patients receiving anthracycline-based chemotherapy. Drugs. 1998 Sep;56(3):385–403.
7. Kasote DM, Katyare SS, Hegde MV, Bae H. Significance of Antioxidant Potential of Plants and its Relevance to Therapeutic Applications. Int J Biol Sci. 2015 Jun 11;11(8):982–91.
8. K S S, Varghese K, Resmi S, Jolly C. Lawsonia Inermis (Henna): A natural dye of various therapeutic uses - A review. Inventi Impact- Cosmeceuticals 2010. 2010 Jun 2; APRIL-SEPT 2010.
9. Rah N, Zahia B, Boucherit K, Benabdallah M, Choukchou-Braham N. Antifungal activity of the Algerian Lawsonia inermis (henna). Pharmaceutical biology. 2012 Nov 6;51.
10. Ali BH, Bashir AK, Tanira MO. Anti-inflammatory, antipyretic, and analgesic effects of Lawsonia inermis L. (henna) in rats. Pharmacology. 1995 Dec;51(6):356–63.
11. Gull I, Sohail M, Aslam MS, Athar MA. Phytochemical, toxicological and antimicrobial evaluation of lawsonia inermis extracts against clinical isolates of pathogenic bacteria. Annals of Clinical Microbiology and Antimicrobials. 2013 Dec 1;12(1):36.
12. Rahman AM, Yusuf SW, Ewer MS. Anthracycline-induced cardiotoxicity and the cardiac-sparing effect of liposomal formulation. Int J Nanomedicine. 2007 Dec;2(4):567–83.
13. A Review: Role of Doxorubicin in Treatment of Cancer | International Journal of Pharmaceutical Sciences And Research. Available from: http://ijpsr.com/bft-article/a-review-role-of-doxorubicin-in-treatment-of-cancer/?view=fulltext
14. Kumar M, Kaur P, Chandel M, Singh AP, Jain A, Kaur S. Antioxidant and hepatoprotective potential of Lawsonia inermis L. leaves against 2-acetylaminofluorene induced hepatic damage in male Wistar rats. BMC Complement Altern Med. 2017 Jan 18;17. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC5242058/
15. Darvin SS, Esakkimuthu S, Toppo E, Balakrishna K, Paulraj MG, Pandikumar P, et al. Hepatoprotective effect of lawsone on rifampicin-isoniazid induced hepatotoxicity in in vitro and in vivo models. Environ Toxicol Pharmacol. 2018 Jul;61: 87–94.
16. Singh DK, Cheema HS, Saxena A, Jyotshana null, Singh S, Darokar MP, et al. Fraxetin and ethyl acetate extract from Lawsonia inermis L. ameliorate oxidative stress in P. berghei infected mice by augmenting antioxidant defence system. Phytomedicine. 2017 Dec 1;36: 262–72.
17. Guha G, Rajkumar V, Kumar RA, Mathew L. Antioxidant Activity of Lawsonia inermis Extracts Inhibits Chromium (VI)-Induced Cellular and DNA Toxicity. Evid Based Complement Alternat Med. 2011;2011. Available from: https://www.ncbi.nlm.nih.gov/ pmc/ articles/ PMC3136683/
18. Abdel-Hamid NM, Mohafez OM, Nazmy MH, Farhan A, Thabet K. The effect of co-administration of Lawsonia inermis extract and octreotide on experimental hepatocellular carcinoma. Environ Health Prev Med. 2015 May;20(3):195–203.
19. Dasgupta T, Rao A, Yadava P. Modulatory effect of Henna leaf (Lawsonia inermis) on drug metabolising phase I and phase II enzymes, antioxidant enzymes, lipid peroxidation and chemically induced skin and forestomach papillomagenesis in mice. Molecular and cellular biochemistry. 2003 Mar 1; 245:11–22.
20. Evaluation of Antiulcer Activity of Lawsonia inermis and Murraya koenigii Seed Extract in Ethanol-induced Gastric Mucosal Damage in Rats [Internet]. Science Alert. Available from: https:// scialert.net/ fulltext/?doi=ajbs.2019.884.890
21. Hegde M, Karki SS, Thomas E, Kumar S, Panjamurthy K, Ranganatha SR, et al. Novel Levamisole Derivative Induces Extrinsic Pathway of Apoptosis in Cancer Cells and Inhibits Tumor Progression in Mice. PLOS ONE. 2012 Sep 10;7(9):e43632.