Author(s): VKK Mandlem, N. Gouri Priya, M. Raghavendra, K. Abbulu


DOI: 10.5958/0974-360X.2020.00579.X   

Address: VKK Mandlem*, N. Gouri Priya, M. Raghavendra, K. Abbulu
CMR College of Pharmacy, Kandlakoya (V), Medchal Road, Hyderabad-501401.
*Corresponding Author

Published In:   Volume - 13,      Issue - 7,     Year - 2020

Background: Cardiovascular diseases are major death causing issue globally. Dietary polyphenols can maintain or enhance cardiovascular health conditions. Every part of tamarind has numerous pharmacological properties. However, so far pericarp (shell) of tamarind fruit which contain polyphenols is not evaluated for cardioprotective activity. Hence the present study was designed to evaluate cardioprotective role of Tamarindus indica Linn pericarpic extract against doxorubicin (DOX) induced cardiotoxicity. Materials and Methods: The hydroalcoholic extract of Tamarind pericarp (HAETP) was prepared by cold maceration and subjected to preliminary phytochemical investigation. Albino wistar rats were randomized into 5 groups (n=6), group I received normal saline as vehicle daily, group II received vehicle + single dose of DOX (20mg/kg body weight i.p) on 29th day, groups III IV and V were pretreated with amlodipine (5mg/kg body weight p.o), HAETI (200 and 400 mg/kg body weight p.o) respectively followed by DOX on 29th day. On 31st day change in body weight, heart weight and serum parameters (LDH, CK-MB, AST, ALT and Calcium) were evaluated. Animals were sacrificed and heart tissue was used to estimate oxidative stress biomarkers (LPO, GSH and CAT), membrane bounded enzymes (Na+/k+ ATPase, Ca++ ATPase and Mg++ ATPase) and histopathological studies. Results: DOX treated rats significantly increased the levels of serum cardiac and oxidative stress biomarkers which were reversed by HAETP treated rats. The alterations caused by DOX in Na+/k+ ATPase, Ca++ ATPase and Mg++ ATPase levels were restored in HAETP treated rats and protected myocardium from toxic effect of DOX. Conclusion: The results reveal that tamarind pericarp has cardioprotective activity which might be due to its antioxidant properties.

Cite this article:
VKK Mandlem, N. Gouri Priya, M. Raghavendra, K. Abbulu. Evaluation of Cardioprotective Activity of Tamarindus indica Linn Pericarpic extract in Doxorubicin induced Cardiotoxicity in Experimental Rats. Research J. Pharm. and Tech. 2020; 13(7): 3267-3273. doi: 10.5958/0974-360X.2020.00579.X

1. Prabhakaran D, Jeemon P, Roy A. Cardiovascular diseases in India: current epidemiology and future directions. Circulation. 2016 Apr 19;133(16):1605-20.
2. Mendis S, Puska P, Norrving B, World Health Organization. Global atlas on cardiovascular disease prevention and control. Geneva: World Health Organization; 2011.
3. Zhang X, Zhu Y, Dong S, Zhang A, Lu Y, Li Y, Lv S, Zhang J. Role of oxidative stress in cardiotoxicity of antineoplastic drugs. Life Sciences. 2019 Jun 3.
4. M. Scherrer-Crosbie. Anti-Cancer Treatments and Cardiotoxicity. Mechanisms, Diagnostic and Therapeutic Interventions 2017, Pages 35-38
5. Grenier, M. A., and Lipshultz, S. E. (1998). Epidemiology of anthracycline cardiotoxicity in children and adults. Seminars in Oncology, 25(4 SUPPL. 10), 72-85
6. Larussi D, Indolfi P, Casale F, et al. Recent Advances in the Prevention of Anthracycline Cardiotoxicity in Childhood. Cur Med Chem. 2001;13:1649-1660.
7. Sterba M et al. Oxidative stress, redox signaling, and metal chelation in anthracycline cardiotoxicity and pharmacological cardioprotection. Antioxidants and Redox Signaling. 2013;18(8):899-929
8. Spallarossa P et al. A recommended practical approach to the management of anthracycline-based chemotherapy cardiotoxicity. Journal of Cardiovascular Medicine. 2016;17:e84-e92
9. Varricchi G et al. Antineoplastic drug-induced cardiotoxicity: A redox perspective. Frontiers in Physiology. 2018;9:167.
10. Koleini N, Nickel BE, Edel AL, Fandrich RR, Ravandi A, Kardami E. Oxidized phospholipids in Doxorubicin-induced cardiotoxicity. Chemico-Biological Interactions. 2019 Jan 29.
11. Sudjaroen Y, Haubner R, Würtele G, Hull WE, Erben G, Spiegelhalder B, Changbumrung S, Bartsch H, Owen RW. Isolation and structure elucidation of phenolic antioxidants from Tamarind (Tamarindus indica L.) seeds and pericarp. Food and Chemical Toxicology. 2005 Nov 1;43(11):1673-82.
12. Bhadoriya, Santosh Singh and Ganeshpurkar, Aditya and Narwaria, Jitendra and Rai, Gopal and Jain, Alok. (2011). Tamarindus indica: Extent of explored potential. Pharmacognosy Reviews. 5. 73-81. 10.4103/0973-7847.79102.
13. Ghosh S, Chatterjee JK, Chalkroborty B, Hazra AK. Comparison of different aqueous extraction methods for optimum extraction of polyphenols and in-vitro anti-oxidant activity from pomegranate peel. Journal of Pharmacognosy and Phytochemistry. 2019; 8(3): 342-7.
14. Ananth V. Pharmacognostical and preliminary phytochemical profile of the leaf extracts of Embelia ribes Burm. F. Journal of Pharmacognosy and Phytochemistry. 2019;8(1):1861-4.
15. 15.Khan G, Haque SE, Anwer T, Ahsan MN, Safhi MM, Alam MF. Cardioprotective effect of green tea extract on doxorubicin-induced cardiotoxicity in rats. Acta Pol Pharm. 2014 Sep 1;71(5):861-868.
16. Lowry OH. Rosebrough, NJ, Farr, AL, and Randall, RJ Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951 Nov;193(1):265-75.
17. Sedlak J., Lindsay R.H.: Anal. Biochem. 25,192 (1968).
18. Claiborne A.: Catalase activity, in Handbook of Methods for Oxygen Radical Research. Greenwald R.A. Ed., pp. 283ñ284, CRC Press, Boca Raton 1985/
19. Fiske, C. H., And Subbarow, Y., J. Biol. Chem., 66, 375
20. Hjerten S, Pan H. Purification and characterization of two forms of a low affinity Ca2+ATPase from erythrocyte membranes. Biochim Biophys Acta 1983;728:281-8.
21. Ohnishi T, Suzuki T, Suzuki Y, Ozawa K. A comparative study of plasma membrane Mg2+ATPase activities in normal, regenerating and malignant cells. Biochim Biophys Acta 1982;684:67-74.
22. Gomathi AC, Rajarathinam SX, Sadiq AM. Phyto chemical screening of aqueous extract of Tamarind (Tamarindus indica L.) Shell. International Journal of Basic and Applied Research. 2017;7(11).
23. M. Santosh Kumar, Viswanatha Swamy Mallinathaiah Agadi Hiremath Cardioprotective effect of Acorus calamus against doxorubicin-induced myocardial toxicity in albino Wistar rats. Indian Journal of Health Sciences, May-Aug 2016, Vol 9, Issue 2.
24. Bhupati, L.V.S., S. Muvvala, and P. Shashank. Protective effect of Vitis vinifera in doxorubicin induced oxidative stress in rats- A preliminary study. International Journal of Advances in Pharmacy Medicine and Bioallied Sciences 2:7-14 (2014)
25. V. Madhava Reddy Papasani, B. Hanumantharayappa, A. Annapurna. Cardioprotective Effect of Naringin Against DoxorubicinInduced Cardiomyopathy in Rats. Indo American Journal of Pharm Research. 2014:4(05).
26. Zhou S, Starkov A, Froberg MK, Leino RL, Wallace KB. Cumulative and irreversible cardiac mitochondrial dysfunction induced by doxorubicin. Cancer Research. 2001 Jan 1;61(2):771-7.
27. J. M. Kulkarni, A. H. M. Viswanatha Swamy Cardioprotective effect of gallic acid against doxorubicin-induced myocardial toxicity in albino rats Indian Journal of Health Sciences, Jan-Jun 2015, Vol 8,Issue 1.
28. Kumar MS, Hiremath VS. Cardioprotective effect of Acorus calamus against doxorubicin-induced myocardial toxicity in albino Wistar rats. Indian j health sci 2016; 9:225-34.
29.  Chalcroft SC, Gavin JB, Herdson PB. Fine structural changes in rat myocardium induced by daunorubicin. Pathology. 1973 Jan 1; 5(2):99-105.

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