Author(s): Pavya, Deeksha. K, Sowmya Hari

Email(s): sowmya.se@velsuniv.ac.in

DOI: 10.5958/0974-360X.2020.00578.8   

Address: Pavya, Deeksha. K, Sowmya Hari*
Department of Bio-Engineering, School of Engineering, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai-600117, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 7,     Year - 2020


ABSTRACT:
Hypertension is a chronic disorder where the force of the blood against artery walls is too high hypertension has several risk factors like heart disease and stroke. Alpha adducin is a hetero dimeric cytoskeleton protein which consists of an alpha subunit and either a beta sub unit or gamma subunit. The mutation in the alpha adducin subunit leads to the stimulation of sodium, potassium, and adenosine triphosphate ATPase activity in the renal tubular cells which leads to hyper tension. The native structure of alpha adducin protein is not determined. Hence the structures of native alpha adducin protein and mutated structure were developed using homology modelling. The three dimensional structures were developed using MODELLER and SWISS model. The structures are validated using procheck. These structures will be fundamental in structure prediction and also for drug discovery.


Cite this article:
Pavya, Deeksha. K, Sowmya Hari. Comparative Modeling of Native and Mutated Structures of Alpha adducin Protein. Research J. Pharm. and Tech. 2020; 13(7): 3262-3266. doi: 10.5958/0974-360X.2020.00578.8


2. REFERENCES:
1. Ji LD, Zhang LN, Shen P, Wang P, Zhang YM, Xing WH, Xu J. Association of angiotensinogen gene M235T and angiotensin-converting enzyme gene I/Dpolymorphisms with essential hypertension in Han Chinese population: a meta-analysis. Journal of Hypertension. 2010 Mar 1;28(3):419-2.
2. Freeman AJ, Vinh A, Widdop RE. Novel approaches for treating hypertension. F1000Research. 2017;6.
3. Ramu P, Umamaheswaran G, Shewade DG, Swaminathan RP, Balachander J, Adithan C. Gly460Trp polymorphism of the ADD1 gene and essential hypertension in an Indian population: A meta-analysis on hypertension risk. Indian Journal of Human Genetics. 2010 Jan;16(1):8.
4. Shikawa K, Katsuya T, Sato N, Nakata Y, Takami S, Takiuchi S, Fu Y, Higaki J, Ogihara T. No association between α-adducin 460 polymorphism and essential hypertension in a Japanese population. American Journal of Hypertension. 1998 Apr 1;11(4):502-6.
5. Sugimoto K, Hozawa A, Katsuya T, Matsubara M, Ohkubo T, Tsuji I, Motone M, Higaki J, Hisamachi S, Imai Y, Ogihara T. Alpha-adducin Gly460Trp polymorphism is associated with low renin hypertension in younger subjects in the Ohasama study. Journal of Hypertension. 2002 Sep 1;20(9):1779-84.
6. Grant FD, Romero JR, Jeunemaitre X, Hunt SC, Hopkins PN, Hollenberg NH, Williams GH. Low-renin hypertension, altered sodium homeostasis, and an α-adducin polymorphism. Hypertension. 2002 Feb 1;39(2):191-6.
7. Manunta P, Burnier M, D’Amico M, Buzzi L, Maillard M, Barlassina C, Lanella G, Cusi D, Bianchi G. Adducin polymorphism affects renal proximal tubule reabsorption in hypertension. Hypertension. 1999 Feb 1;33(2):694-7.
8. Kalpana J, Vivek P, SanthanaBharathi N, Sathishpandiyan S, Prathap S, Karunakaran S, Saravanan A. Insilico Structural and Functional Analysis of Nitrogen Fixing Proteins from Trichodesmiumthiebautii.
9. Satyanarayana SD, Krishna MS, Kumar PP, Jeereddy S. In silico structural homology modeling of nifA protein of rhizobial strains in selective legume plants. Journal of Genetic Engineering and Biotechnology. 2018 Jul 7.
10. Eswar N, Webb B, Marti‐Renom MA, Madhusudhan MS, Eramian D, Shen MY, Pieper U, Sali A. Comparative protein structure modeling using Modeller. Current Protocols in Bioinformatics. 2006 Sep 1;15(1):5-6.
11. Jones DT. Progresss in prediction. Current Opinion in Structural Biology. 1997 Jun 1;7(3):37787
12. Fiser A, Šali A. Modeller: generation and refinement of homology-based protein structure models. In Methods in enzym BLAST G. PSI-BLAST: a new generation of protein database search programs Altschul. Stephen F. 1997:3389-402.ology 2003 Jan 1 (Vol. 374, pp. 461-491).
13. .BLAST G. PSI-BLAST: a new generation of protein database search programs Altschul. Stephen F. 1997:3389-402.
14. Vriend G. WHAT IF: a molecular modeling and drug design program. Journal of molecular graphics. 1990 Mar 1;8(1):52-6.
15. Pymol T. The PyMOL molecular graphics system.
16. Eswar N, Webb B, Marti-Renom MA, Madhusudhan MS, Eramian D, Shen MY, Pieper U, Sali A. Comparative protein structure modeling using Modeller. Curr Protoc Bioinformatics. 2006 Oct; Chapter 5:Unit-5.6. doi: 10.1002/0471250953.bi0506s15. PMID: 18428767; PMCID: PMC4186674.
17. Laskowi RA, MacArthur MW, Moss DS, Thornton JM. PROCHEK: a program to check the stereochemical quality of protein structures. Journal of Applied Crystallography. 1993 Apr 1;26(2):283-91.
18. Hollingsworth SA, Karplus PA. A fresh look at the Ramachandran plot and the occurrence of standard structures in proteins. Biomolecular Concepts. 2010 Oct 1;1(3-4):271-83.
19. Fiser A, Sali A. ModLoop: automated modeling of loops in protein structures. Bioinformatics. 2003 Dec 12;19(18):2500-1.
20. Schwede T, Kopp J, Guex N, Peitsch MC. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Research. 2003 Jul 1;31(13):3381-5.
21. Teggi R, Lanzani C, Zagato L, Carpini SD, Manunta P, Bianchi G, Bussi M. Gly460Trp α-Adducin mutation as a possible mechanism leading to endolymphatic hydrops in Ménière's syndrome. Otology and Neurotology. 2008 Sep 1;29(6):824-8.
22. Butt AM, Ahmed A. MUTATER: Tool for the Introduction of Custom Position Based Mutations in Protein and Nucleotide Sequences. J. Proteomics Bioinform. 2009;2:344-8.
23. Dhanik A, McMurray JS, Kavraki LE. DINC: a new AutoDock-based protocol for docking large ligands. BMC structural biology. 2013Nov;13(1):S11. Muhammad SA, Fatima N.
24.  In silico analysis and molecular docking studies of potential angiotensin-converting enzyme inhibitor using quercetin glycosides. Pharmacognosy Magazine. 2015 May;11(Suppl 1):S123

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