Author(s): Dina Dewi S.L. Ismail, Retty Ratnawati, Handono Kalim, Karyono Mintaroem, Efris Kartika Sari, Shila Wisnasari


DOI: 10.5958/0974-360X.2020.00543.0   

Address: Dina Dewi S.L. Ismail1,2*, Retty Ratnawati3, Handono Kalim4, Karyono Mintaroem5, Efris Kartika Sari2, Shila Wisnasari2
1Post Graduate Doctoral Program of Medical Science of Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
2Department of Nursing, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
3Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
4Department of Internal Medicine, Faculty of Medicine, University Brawijaya, Malang, Indonesia.
5Department of Pathology Anatomy, Faculty of Medicine, University Brawijaya, Malang, Indonesia.
*Corresponding Author

Published In:   Volume - 13,      Issue - 7,     Year - 2020

Second-degree burn is one of acute traumas that has complexities in its healing process; one of which is the disruption of neutrophils activity and function as the initial cellular defense during inflammatory phase. Appropriate wound treatment can modulate the quantity of neutrophils in burn wounds. Cyclea barbata Miers (CBM) is a local plant that has a potential to influence inflammatory process. This study was aimed to determine the polyphenol and monosaccharide contents of CBM and the effect of CBM on neutrophils migration and apoptosis. We used 30 adult male Wistar rats as experimental animals, wich were randomly separated in two groups according the eutanized time. Each group divided in 3 groups (n=5 per group). Cyclea barbata Miers’ content was analyzed using liquid chromatography mass spectrometry (LCMS/MS) method, while neutrophils migration and apoptosis were observed using immunofluorescence assay method. The result of LCMS/MS assay showed that CBM contains isoquercetin (93.83ng/mL) and d-galacturonic acid (0.114ng/mL). Further result showed that CBM extract can decrease neutrophil migration and improve neutrophil apoptosis during the inflammatory phase of the burn wound. This ability may be enabled by the anti-inflammatory and antiradical properties of isoquercetin and DGA in Cyclea barbata Miers. Further study is required to confirm this hypothesis, specially addition of comparasion groups that treated by single compound such as isoquercetin and DGA.

Cite this article:
Dina Dewi S.L. Ismail, Retty Ratnawati, Handono Kalim, Karyono Mintaroem, Efris Kartika Sari, Shila Wisnasari. The Sinergy of Isoquercetin and D-Galacturonic acid of Cyclea barbata Miers extract on the Migration and Apoptosis of Neutrophil in Rat Burn Wound. Research J. Pharm. and Tech. 2020; 13(7): 3059-3064. doi: 10.5958/0974-360X.2020.00543.0


1.       Baskaran H, Yarmush ML, Berthiaume F. Dynamics of Tissue Neutrophil Sequestration after Cutaneous Burns in Rats. J Surg Res. 2000; 93(1):88–96.

2.       Bohannon J, Cui W, Sherwood E, Toliver-Kinsky T. Dendritic cell modification of neutrophil responses to infection after burn injury. J Immunol Baltim Md 1950. 2010; 185(5):2847–53.

3.       Butler KL, Ambravaneswaran V, Agrawal N, Bilodeau M, Toner M, Tompkins RG, et al. Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices. PLOS ONE. 2010; 5(7): e11921.

4.       Jones CN, Moore M, Dimisko L, Alexander A, Ibrahim A, Hassell BA, et al. Spontaneous Neutrophil Migration Patterns during Sepsis after Major Burns. Moldawer LL, editor. PLoS ONE. 2014; 9(12): e114509.

5.       Merlin NJ Parthasarathy, Manavalan R, Devi P, Meera R. Apoptosis Significance and Molecular Mechanisms- A Review. Asian J Res Chem. 2009; 2(4):369–75.

6.     Neely C, Wolfgang M, Vadlamudi S, Maile R, Cairns B. Increased neutrophil migration rescues impaired function and susceptibility to Pseudomonas pneumonia after burn injury (56.25). J Immunol. 2011; 186(1 Supplement):56.25-56.25.

7.       Jawad BO. Apoptosis Study on the Normal and Diabetics Type 2 Patients. Res J Pharm Technol. 2018; 11(6):2493–5. 

8.       Hirth DA, Singer AJ, Clark RAF, McClain SA. Histopathologic staining of low temperature cutaneous burns: Comparing biomarkers of epithelial and vascular injury reveals utility of HMGB1 and hematoxylin phloxine saffron. Wound Repair Regen. 2012; 20(6):918–27.

9.       Beyrau M, Bodkin JV, Nourshargh S. Neutrophil heterogeneity in health and disease: a revitalized avenue in inflammation and immunity. Available from: https://www.ncbi.nlm.nih.govvolume-and-issues/2012/vol-2-issue-11

10.    Silvestre-Roig C, Hidalgo A, Soehnlein O. Neutrophil heterogeneity: implications for homeostasis and pathogenesis. Blood. 2016; 127(18):2173–81.

11.    Alaghari S, Sailesh KS. Association of Neutrophil/Lymphocyte (N/L) Ratio and Spatial and Verbal Memory in Females. Res J Pharm Technol. 2018; 11(12):5337–9.

12.    Pase L, Layton JE, Wittmann C, Ellett F, Nowell CJ, Reyes-Aldasoro CC, et al. Neutrophil-delivered myeloperoxidase dampens the hydrogen peroxide burst after tissue wounding in zebrafish. Curr Biol CB. 2012; 22(19):1818–24.

13.    Ipaktchi K, Mattar A, Niederbichler AD, Hoesel LM, Hemmila MR, Su GL, et al. TOPICAL p38MAPK Inhibition Reduces Dermal Inflammation and Epithelial Apoptosis In Burn Wounds: Shock. 2006; 26(2):201–9.

14.    NasimunIslam N1, Jemimah Naine S, Shaik Jameel, Khora SS, Venkat Kumar, Mohanasrinivasan V, et al. Cytotoxic Property of Cocos nucifera shell Extracts on HeLa Cells. Res J Pharm Technol. 2014; 7(5):521–5.

15.    Mahadi R, Rasyiid M, Dharma KS, Anggraini L, Nurdiyanti R, Nuringtyas TR. Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro. J Trop Biodivers Biotechnol. 2018; 3(3):73–9.

16.    Nurlela J. The effect of leaf green grass jelly extract (cyclea l. barbata miers) to motility in mice balb/c male that exposed smoke. J Major. 2015; 4(4).

17.    Djaman Q. Pengaruh Air Perasan Daun Cyclea Barbata Miers (Cincau Hijau) Terhadap Konsentrasi Hcl Lambung Dan Gambaran Histopatologik Lambung Tikus Galur Wistar Yang Diinduksi Acetylsalicylic acid The Effect of Cincau Hijau Leaves Aqueous (Cyclea barbata Miers) on Acetylsalicylic acid-Induced Gastric Acid And Gastrohistopathology Feature In Rats. Program Pasca Sarjana Universitas Diponegoro; 2008.

18.    Katrin, Elya B, Shodiq AM. Aktivitas Antioksidan Ekstrak Dan Fraksi Daun Cincau Hijau Rambat (Cyclea barbata Miers.) Serta Identifikasi Golongan Senyawa Dari Fraksi Yang Paling Aktif. J Bahan Alam Indones. 2012; 8(2):12–9.

19.    Kusmardiyani S, Insanu M, Asyhar MA. Effect A Glycosidic Flavonol Isolated from Green Grass Jelly (Cyclea Barbata Miers) Leaves. Procedia Chem. 2014; 13:194–7.

20.    Mackaman P, Tangsuphoom N, Chavasit V. Effect of extraction condition on the chemical and emulsifying properties of pectin from Cyclea barbata Miers leaves. Int Food Res J [Internet]. 2014 [cited 2017 Oct 8]; 21(2). Available from: http://

21.    Dina Dewi S L I, Wibi Riawan, Ika Setyo Rini, Titin Andri Wihastuti, Ika Fitri Aprilianti, Retty Ratnawati, Et Al. Effects Of Ethanol Cincau (Cyclea barbata Miers) Leaves Extract On Macrophage Migration On Burn Wound.Available from: Madaghiele M, Demitri C, Sannino A, Ambrosio L. Polymeric hydrogels for burn wound care: Advanced skin wound dressings and regenerative templates. Burns Trauma. 2014; 2(4):153–61

22.    Madaghiele M, Demitri C, Sannino A, Ambrosio L. Polymeric hydrogels for burn wound care: Advanced skin wound dressings and regenerative templates. Burns Trauma. 2014; 2(4):153–61.

23.    Krishnasamy L, Selvam MM, Ravikrishnan B. Anticancer property of Colchicine isolated from Indigofera aspalathoids. Res J Pharm Technol. 2016; 9(4):386–90.

24.    Francescato LN, Debenedetti SL, Schwanz TG, Bassani VL, Henriques AT. Identification of phenolic compounds in Equisetum giganteum by LC–ESI-MS/MS and a new approach to total flavonoid quantification. Talanta. 2013; 105:192–203.

25.    Dahlan M. Statistik Untuk Kedokteran Dan Kesehatan Deskriptif, Bivariat dan Multivariat Dilengkapi dengan Menggunakan SPSS. Jakarta: Salemba Medika; 2009.

26.    Sarjono H, Julianita W. SPSS vs LISREL: Sebuah Pengantar, Aplikasi untuk Riset. Jakarta: Salemba Empat; 2011.

27.    Valentová K, Vrba J, Bancířová M, Ulrichová J, Křen V. Isoquercitrin: pharmacology, toxicology, and metabolism. Food Chem Toxicol Int J Publ Br Ind Biol Res Assoc. 2014; 68:267–82.

28.    Paulke A, Eckert GP, Schubert-Zsilavecz M, Wurglics M. Isoquercitrin provides better bioavailability than quercetin: comparison of quercetin metabolites in body tissue and brain sections after six days administration of isoquercitrin and quercetin. Pharm- Int J Pharm Sci. 2012; 67(12):991–996.

29.    Chen M, Dai L-H, Fei A, Pan S-M, Wang H-R. Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro. Exp Ther Med. 2017; 13(4):1353–9.

30.    Arkarapanthu A, Chavasit V, Sungpuag P, Phuphathanaphong L. Gel extracted from Khruea-ma-noi (Cyclea barbata Miers) leaves: chemical composition and gelation properties. J Sci Food Agric. 2005; 85(10):1741–9.

31.    Waldeck H, Wang X, Joyce E, Kao WJ. Active leukocyte detachment and apoptosis/necrosis on PEG hydrogels and the implication in the host inflammatory response. Biomaterials. 2012; 33(1):29–37.

32.    Pagare MS, Joshi H, Patil L, Kadam VJ. Human Milk: Excellent Anticancer Alternative. Res J Pharm Technol. 2012; 5(1):14–9.

33.    Törkvist L, Månsson P, Raud J, Larsson J, Thorlacius H. Role of CD18-Dependent Neutrophil Recruitment in Skin and Intestinal Wound Healing. Eur Surg Res. 2001; 33(4):249–54.

34.    Park S-Y, Kim K-B, Ahn S-H, Kim H-H. The effects of SM-215 on androgeneticalopecia. Res J Pharm Technol. 2018; 11(5):1745–51.

35.    Goodwin NS, Spinks A, Wasiak J. The efficacy of hydrogel dressings as a first aid measure for burn wound management in the pre-hospital setting: a systematic review of the literature: The efficacy of hydrogel dressings as a first aid measure for burns in the pre-hospital setting. Int Wound J. 2016; 13(4):519–25.

36.    Izuegbuna O, Otunola G, Bradley G. Chemical composition, antioxidant, anti-inflammatory, and cytotoxic activities of Opuntia stricta cladodes. PLOS ONE. 2019; 14(1): e0209682.

37.    de Oliveira S, Rosowski EE, Huttenlocher A. Neutrophil migration in infection and wound repair: going forward in reverse. Nat Rev Immunol. 2016; 16(6):378–91.

38.    Juliprihanto A, Hendrawan VF, Wulansari D, Oktanella Y, Widjiati. Placental Cells Number and Caspase-9 Expression in White Rat (Rattus norvegicus) apoptosis exposed with Carbon Black. Res J Pharm Technol. 2019; 12(4):1935–42.

39.    Soebagjo HD, Fatmariyanti S, Sugianto P, Purwanto B, Bintoro UY, Kusumowidagdo ER. Detection of the Calcium and ATP Role in Apoptosis of Retinoblastoma Culture Cells through Caspase-3 Expression. Res J Pharm Technol. 2019; 12(3):1307–14.

40.    Cohen MJ, Brohi K, Calfee CS, Rahn P, Chesebro BB, Christiaans SC, et al. Early release of high mobility group box nuclear protein 1 after severe trauma in humans: role of injury severity and tissue hypoperfusion. Crit Care. 2009; 13(6): R174.

41.    Liu J-J, Song C-W, Yue Y, Duan C-G, Yang J, He T, et al. Quercetin inhibits LPS-induced delay in spontaneous apoptosis and activation of neutrophils. Inflamm Res. 2005; 54(12):500–7.

42.    Sumitha R, Parvathi VD, Banu N. Cytotoxicity Testing of Star Fish Stellaster equestris Extracts on Pa1 Celline. Res J Pharm Technol. 2017; 10(9):2851–6.

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