ABSTRACT:
The renoprotective effect of standardized hydroalcoholic extract of Corallocarpus epigaea was evaluated against cisplatin induced nephrotoxicity. The evaluation was performed on 36 Male Wistar rats. The acute toxicity study was performed in accordance with OECD 425 guidelines. Considering the safety of the extract the screening was done at three dose levels of 100, 200, 400mg/kg. The study was carried for period of 15 days. Vitamin E (100mg/kg) was used as a Standard nephroprotective agent. The kidney function test like serum uric acid, creatinine, albumin, (BUN) was performed. Oxidative stress studies like superoxide dismutase, malondialdehyde activity along with histopathological examination of kidneys was also conducted. Serum creatinine and Urea were elevated in Cisplatin treated group. Decrease in serum albumin was noted in cisplatin control as compared to normal control. Corallocarpus epigaea (100, 200, 400mg/kg) and Vitamin E (100mg/kg) decreased the serum creatinine and BUN and increased the serum albumin level. Decrease in activity of superoxide dismutase was observed in the Cisplatin group as compared to control. Corallocarpus epigaea (100, 200, 400mg/kg) and Vitamin E (100mg/kg) increased superoxide dismutase levels and decreased malondialdehyde levels as compared to Cisplatin group. Histopathological examination of the kidneys revealed the amelioration of Cisplatin induced renal tubular necrosis. Dose dependant increase in the activity was observed. Present investigation revealed that Corallocarpus epigaea resulted in attenuation of Cisplatin induced renal damage in rats.
Cite this article:
Amruta V. Yadav, Mohd. Bashar Momin, Md. Awais Panjwani. Renoprotective effect of Corallocarpus epigaea in Nephropathy in Wistar Rats. Research J. Pharm. and Tech. 2020; 13(7): 3163-3168. doi: 10.5958/0974-360X.2020.00559.4
Cite(Electronic):
Amruta V. Yadav, Mohd. Bashar Momin, Md. Awais Panjwani. Renoprotective effect of Corallocarpus epigaea in Nephropathy in Wistar Rats. Research J. Pharm. and Tech. 2020; 13(7): 3163-3168. doi: 10.5958/0974-360X.2020.00559.4 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-7-19
REFERENCES:
1. Skinner R. Nephrotoxicity-what do we know and what don't we know. Journal of Pediatric Hematology/Oncology. 2011; 33(2):128-34.
2. Hoste EA, Kellum JA. Acute kidney injury: epidemiology and diagnostic criteria. Current Opinion in Critical Care. 2006; 12(6):531-7.
3. Sahu BD et al. Ameliorative effect of fisetin on cisplatin induced nephrotoxicity in rats via modulation of NF-kB activation and antioxidant defense. Plos One. 2014; 9 (9): 1-15.
4. Kuhlmann MK et al. Insights into potential cellular mechanisms of cisplatin nephrotoxicity and their clinical application. Nephrol Dial Transplant.1997;12: 2478-80.
5. Arany I, Safirstein RL. Cisplatin nephrotoxicity. Semin Nephrol. 2003; 23:460-4.
6. Chatterjee P et al. Protective effects of the aqueous leaf extract of Aloe barbadensis on gentamicin and cisplatin induced nephrotoxic rats. Asian Pac J Trop Dis. 2012; 1754-63.
7. Hayati F et al. Prevention of cisplatin nephrotoxicity. J Nephropharmacol. 2016; 5(1): 57-60.
8. Kirtikar KR and Basu BD. Indian Medicinal Plants. Lalit Mohan Basu Co. Allahabad. 1984.
9. Gnananath K et al. Evaluation of antidiabetic activity in Corallocarpus epigaeus rhizomes. International Current Pharmaceutical Journal. 2013 ;2(3):53-6.
10. 10.Guideline OECD 425: acute oral toxicity-up-and-down procedure. OECD Guidelines for the Testing of Chemicals. 2001; 2:12-6.
11. Yousef MI et al. Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food and Chemical Toxicology. 2009; 47 (6): 1176-83.
12. Debnath S et al. Nephroprotective evaluation of ethanolic extract of the seeds of papaya and pumpkin fruit in Cisplatin-induced Nephrotoxicity. Journal of Pharmaceutical Science and Technology. 2010; 2 (6): 241-6.
13. Rajagopal G and Toora BD. Practical Biochemistry. Ahuja publishing house. New Delhi.2004.
14. Slater TF, Sawyer BC. The stimulatory effects of carbon tetrachloride and other halogenoalkanes on peroxidative reactions in rat liver fractions in vitro. General features of the systems used. Biochemical Journal. 1971;123(5):805-14.
15. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological chemistry. 1972 ;247(10):3170-5.
16. Bancroft D et al. Theory and Practice of Histological techniques. Churchill Livingstone, Edinburgh, London, Melbourne. 1996.
17. Dutta D, Harikumar SL, Grewal AK. Role of Herbal Drugs in Nephrotoxicity and Neurological Disorder: Connecting a Bridge between Kidney and Brain. Asian Journal of Research in Chemistry. 2013;6(10):1165.
18. Abhirama BR, Rajagopal SS, Nanjan M. Nephroprotective effect of ethanol extract of Biophytum sensitivum (Linn.) DC in cisplatin-induced experimental renal damage in rats. Research Journal of Pharmacy and Technology. 2017 ;10(6):1772-9.
19. Ilic S et al. Protective effect of quercetin on cisplatin induced nephrotoxicity in rats. Facta Universitatis, Series: Medicine and Biology. 2014; 16(2):71-5.
20. Mugada V et al. Assessment of Ototoxicity and Nephrotoxicity in patients receiving weekly Cisplatin Chemotherapy: A Prospective Observational Study. Research Journal of Pharmacy and Technology. 2019;12 (4):1922-6.
21. Salam AB et al. Protective Effect of Ixora Coccinea Flowers against Cadmium Chloride induced Nephrotoxicity Model in Rats. Research Journal of Pharmacy and Technology. 2018 1;11(11): 4949-52.
22. Sathyanarayanan G, Shanmugasundaram P, Geetha P. A Prospective Observational Study on Microalbuminuria as Risk factor of Chronic Renal Failure in patients with Type 2 Diabetes. Research Journal of Pharmacy and Technology. 2017;10(9):3085-8
23. Sherif MH, Abas AS, Zaitoun LA. Ginger extract protect Iodinated Contrast Media Nephrotoxicity in rats through modulation of Oxidative Stress, Cystain C, NGAL and TNF α. Research Journal of Pharmacy and Technology. 2018 ;11(12):5439-48.
24. Viswanathan V et al. Serum albumin levels in different stages of type 2 diabetic nephropathy patients. Indian J Nephrol. 2004; 14: 89-92.
25. Lakshmana G et al. Study of Antidiabetic and Free Radical Scavenging Activity of the Methanolic and N-Hexane Extract of Asystasia gangetica Leaf in Alloxan Induced Diabetic Rats. Research journal of Pharmacology and Pharmacodynamics. 2014; 6(2):86.
26. Vaidegi N, Anuradha R. Antinephrotoxic Effect of Boerhaavia diffusa L. Root on Lead Acetate Induced Nephrotoxicity in Male Albino Rats. Research Journal of Pharmacology and Pharmacodynamics. 2012 ;4(6):2.
27. Azize SW. Study of Heavy Metals and their effects on Oxidant/Antioxidant Status in Workers of fuel Station in Hilla city-Iraq. Research Journal of Pharmacy and Technology. 2018; 11(1):312-6.
28. Gautam RK et al. Evaluation of nephroprotective activity of Mentha Arvensis in cisplatin induced nephrotoxicity. Asian J Pharm Clin Res. 2014; 7 (4):188-91.
29. Padmaja M et al. Therapeutic Efficiency of Leucas aspera (Lamiaceae) on Lead Acetate Induced Nephrotoxicity and Oxidative Stress in Male Wistar Rat. Asian J. Research Chem. 2011; 4(10): 186-5.