Studies on the Anti-inflammatory potential of selected medicinal plants in vitro

K. Kousalya; T. Priya; P. Venkatalakshmi

doi:10.5958/0974-360X.2020.00556.9

Research Journal of Pharmacy and Technology

ISSN

0974-360X (Online)
0974-3618 (Print)

Submit Article

Studies on the Anti-inflammatory potential of selected medicinal plants in vitro

Author(s): K. Kousalya, T. Priya, P. Venkatalakshmi

Email(s): venkatalakshmisathish@gmail.com

DOI: 10.5958/0974-360X.2020.00556.9

Address: K. Kousalya1, T. Priya1, P. Venkatalakshmi1,2
1PG Research Department of Biochemistry, STET Women’s College, Mannargudi.
2Department of Biochemistry, PSG College of Arts and Science, Coimbatore.
*Corresponding Author

Published In: Volume - 13, Issue - 7, Year - 2020

View HTML

View PDF

ABSTRACT:
Chronic inflammation is the underlying factor in causing multitude of human ailments ranging from arthritis to cancer. Amidst the emerging synthetic drugs, people started re-evaluating traditional plant based medicines because of the efficacy and safety of herbal medicine. Hence in the present study, efforts are made to validate the anti-inflammatory potential of four medicinal plants using in-vitro procedures. Ten different concentrations of aqueous extracts of selected medicinal plants ranging from 0.01-10mg/ml were taken. At a concentration of 10 mg/ml Erythrina indica has shown 74.15% of protein denaturation inhibition followed by Mukia maderaspatana (53%). Cardiospermum halicacabum has shown only 42.40% activity, whereas Delonix elata has shown only 14% activity. In the case of proteinase inhibition assay, Mukia maderaspatana at a concentration of 10 mg/ml has exhibited 74% inhibition of proteinase followed by Cardiospermum halicacabum 58%, Erythrina indica 38% and Delonix elata 30%. Among the four plants selected, except Delonix elata all the other three plants have revealed remarkable anti-inflammatory activity.

Keywords:

Cite this article:
K. Kousalya, T. Priya, P. Venkatalakshmi. Studies on the Anti-inflammatory potential of selected medicinal plants in vitro. Research J. Pharm. and Tech. 2020; 13(7): 3147-3150. doi: 10.5958/0974-360X.2020.00556.9

Cite(Electronic):
K. Kousalya, T. Priya, P. Venkatalakshmi. Studies on the Anti-inflammatory potential of selected medicinal plants in vitro. Research J. Pharm. and Tech. 2020; 13(7): 3147-3150. doi: 10.5958/0974-360X.2020.00556.9 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-7-16

REFERENCES:

1. Chatterjee P, Chandra S, Dey P, Bhattacharya S. Evaluation of anti-inflammatory effects of green tea and black tea: A comparative in vitro study. J Adv Pharm Tech Res 2012; 3:136-8.

2. Tripathi KD. Essentials of Medical Pharmacology. 6th Ed. New Delhi: Jaypee Brother’s Medical Publishers (P) Ltd.; 2008.

3. Sivaranjani C, Venkatalakshmi P, and Brindha P. In vitro anti-inflammatory and antioxidant activity on fruits of Terminalia catappa L. Res. J. Pharm. Tech., 2015; 8(10): 1409-1411.

4. Padmanabhan P, and Jangle SN. Evaluation of in vitro Antioxidant activity of Herbal preparation. A combination of four medicinal plants. International Journal Basic and Applied Medicinal Science. 2012; 2(1): 109-116.

5. Venkatalakshmi P, Brindha P, and Vadivel V, 2014. In vitro antioxidant and anti-inflammatory studies on fruits of Terminalia catappa. Int J Phytomed. 2015; 7 (3): 246-253.

6. Divya, RS, Venkatalakshmi P, Vadivel, V, and Brindha P. In vitro studies on the biological activities of flowers of banana (Musa paradisiaca L.,). Der Pharmacia Letter. 2016; 8(10): 238-246.

7. Karthika, T, Venkatalakshmi, P, Vadivel V, and Brindha P. In vitro Anti-Obesity, Antioxidant and Anti-Inflammatory Studies on the Selected Medicinal Plants. International Journal of Toxicological Research. 2016; 8(5):332-340.

8. Raman N, Radha A, and Ravi M. Antibacterial activity of Cardiospermum halicacabum against human and plant pathogens. Indian Drugs. 1998; 35: 29–31.

9. Rao V, Chandra PNK, and Shanta KSM. Pharmacological investigation of Cardiospermum halicacabum (Linn) in different animal models of diarrhoea. Indian J. Pharm., 2006; 38: 346-349.

10. Kumaran A, and Karunakaran RJ. Antioxidant activities of the methanol extract of Cardiospermum halicacabum. J Pharm Biol. 2006; 44:145-151.

11. Babu V, and Krishnakumari KCS. Cardiospermum halicacabum suppresses the production of TNF-alpha and nitric oxide by human peripheral blood mononuclear cells. Afr. J. Biomed. 2006; 9: 95–99.

12. Sheeba MS, and Asha VV. Effect of Cardiospermum halicacabumon ethanol induced gastric ulcers in rats. J. Ethnopharmacol. 2006; 106:105–110

13. Gopalakrishnan C, Dhannanjayan R, and Kameshwaran L. Studies on the pharmacological actions of Cardiospermum halicacabum. Indian J. Physiol. Pharmacol. 1976; 20: 203–208.

14. Abdulla, P. In: Flora of West Pakistan. (Eds.): E. Nasir and S.I. Ali. Islamabad, Pakistan. 1973; pp. 39: 1-8.

15. Nadkarni KM. Indian Materia Medica. Popular Book Depot, Bombay. 1976;271.

16. Chopra RN, Nayar SL, and Chopra IC. Glossary of Indian Medicinal Plants, New Delhi; Council for Scientific Ind. Res. 1980; 51-55.

17. Joshi SK, Dhstms BD, Bhatia CR, Singh RV, and Thakur RS. The Wealth of India Raw Materials. New Delhi; Council of Scientific Ind Res Pub. 1992; 3:270-271.

18. Muthumani P, Meera R, Venkatraman S, Ganapathy S, and Devi P. Study of phytochemical, analgesic and anti ulcer activity of extracts of aerial parts of Cardiospermum halicacabum Linn., IJPSR.2010; 1(10):128-137.

19. Waako PJ, Gumede B, Smith P and Folb PI. 2005. The in vitro and in vivo antimalarial activity of Cardiospermum halicacabum L. and Momordica foetida Schumch. Et Thonn. J. Ethnopharmacol. 2005; 99(1):137-143.

20. Khunkitti W, Fujimaki Y, and Aoki Y. In vitro antifilarial activity of extracts of the medicinal plant Cardiospermum halicacabum against Brugia pahangi. J. Helminthol. 2000; 74(3): 241-246.

21. Asha VV, and Pushpangadan P. 1999. Antipyretic activity of Cardiospermum halicacabum. Indian J. Exp. Biol.1999; 37(4): 411-414.

22. Chinchawade AB, Deshmukh DB, Gaikwad DD, Grampurohit ND. Anticonvulsant activity of chloroform extract of bark and root of Erythrina variegata L. International Journal of Pharmaceutical and Clinical Research.2013; 5. 23-25.

23. Tanaka H, Sato M, Fujiwara S, Hirata M, Etoh H and Takeuchi H. Antibacterial activity of isoflavonoids isolated from Erythrina variegata against methicillin-resistant Staphylococcus aureus. Lett. App. Microbio. 2002; 35: 494-498.

24. Venkatesan D and Karrunakaran CM. Anti microbial activity of selected Indian Medicinal Plants. Journal of Phytology. 2010; 2(2): 44-48.

25. Liu IX, Durham DG, and Richards ME. Vancomycin resistance reversal in Enterococci by flavonoids. Journal of Pharmacy and Pharmacology. 2001; 53: 129-132.

26. Veeramani C, Aristatle B, Pushpavalli G, and Pugalendi KV. Effects of Melothria maderaspatana leaf extract on antioxidant status in sham-operated and uninephrectomized DOCA-salt hypertensive rats. Saudi J Biol Sci. 2011; 18 (1): 99-105.

27. Wani VK, Dubey RD, Verma S, Sengottuvelu S and Sivakumar T. Antidiabetic activity of methanolic root extract of Mukia maderaspatana in Alloxan induced diabetic rats. Int J Pharm Technol Res. 2011; 3: 214-220.

28. Balaraman AK, Singh J, Dash S, and Maity TK. Antihyperglycemic and hypolipidemic effects of Melothria maderaspatana and Coccinia indica in streptozotocin induced diabetes in rats, Saudi Pharmac J. 2010; 18 (3): 173-178.

29. Thabrew MI, Gove CD, Hughes RD. McFarlane IG and Williams R. Protection against galactosamine and tert-butyl hydroperoxide induced hepatocyte damage by Melothria maderaspatana extract. Phytother Res. 1995; 9 (7): 513-517.

30. Sasi Priya G, Radhika R, and Siddhuraju P. Antioxidant and antimicrobial activity of traditional Indian leafy vegetables: Mukia maderaspatana and Solanum trilobatum. Int J Pharm Pharm Sci. 2012; 4 (2), 513-521.

31. Krishna RRV, Ganapathy P, Mallikarjuna R, and Ganga RB. Antiinflammatory activity of the leaves and bark of Delonix elata. Ancient Sci Life. 1997; 17(2): 141-143.

32. Evans WC, and Trease. Pharmacognosy. 15th edition, W.S Sauder company Ltd. London. 2002; 137-139, 230-240.

33. Khandelwal KR. Practical pharmacognosy, Techniques and experiment Nirali Prakashan (Pune), 13th edition. 2005.

34. Kokate CK, Purohit AP, and Gokhale SB. A textbook of pharmacognosy, Nirali Prakashan., 2009;27-28.

35. Osman NI, Sidik NJ, Awal A, Adam NAM, and Rezali NI. In vitro xanthine oxidase and albumin denaturation inhibition assay of Barringtonia racemosa L. and total phenolic content analysis for potential anti-inflammatory use in gouty arthritis. Journal of Intercultural Ethnopharmacology. 2016; 5 (4): 343-349.

36. Leelaprakash G, and Mohan Dass S. In vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. International Journal of Drug Development and Research. 2011; 3 (3): 189-196.

37. Sangita Chandra, Priyanka Chatterjee, Protapaditya Dey, Sanjib Bhattacharya, et al. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pacific Journal of Tropical Biomedicine, 2012; 178- 180.

38. Opie EL. On the relation of necrosis and inflammation to denaturation of proteins. J. Exp. Med. 1962; 115: 597-608.

39. Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag BN, Iputo JE. An experimental evaluation of Albuca setosa aqueous extract on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J. Med Plants Res. 2010; 4: 789-795.

40. Govindappa M, Naga Sravya S, Poojashri MN, Sadananda TS, Chandrappa CP. Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extract and active phytochemical screening of Wedelia trilobata (L.). Journal of Pharmacognosy and Phytotherapy, 2011; 3: 3-51.