Development, Characterization and In vitro Evaluation of Donepezil solid Lipid Nanoparticles

Manjunath Kopparam; Suresh V Kulkarni; Shivu SN

doi:10.5958/0974-360X.2020.00551.X

Research Journal of Pharmacy and Technology

ISSN

0974-360X (Online)
0974-3618 (Print)

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Development, Characterization and In vitro Evaluation of Donepezil solid Lipid Nanoparticles

Author(s): Manjunath Kopparam, Suresh V Kulkarni, Shivu SN

Email(s): manju_kop@yahoo.com

DOI: 10.5958/0974-360X.2020.00551.X

Address: Manjunath Kopparam*, Suresh V Kulkarni, Shivu SN
Department of Pharmaceutics, Sree Siddaganga College of Pharmacy, Tumkur - 572102, Karnataka, India.
*Corresponding Author

Published In: Volume - 13, Issue - 7, Year - 2020

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ABSTRACT:
Solid lipid nanoparticles (SLNs) loaded with donepezil were prepared by hot homogenization followed by probe ultrasonication technique. Donepezil SLNs were composed of lipids (1 to 5 %w/v) such as trimyristin, tristearin, glycerol monostearate and compritol which were stabilized by soya lecithin (0.5 to 2.5 %w/v) and poloxamer 188 (0.5 to 2.5 %w/v). Developed donepezil SLNs were characterized for size, zeta potential, entrapment efficiency and content uniformity. Selected method of preparation was able to produce nanoparticles of size range 102.5 to 179.3 nm possessing zeta potential -23.5 to -34.9 mV with entrapment efficiency 86.65 to 89.64 %. FTIR and DSC studies showed that there was no interaction between donepezil and selected lipids. Sterilization by autoclave increased size of SLNs from 1.5 to 1.9 times, however, there was no decrease in the zeta potential of the formulations. Short term stability studies showed that size of SLNs maximum increased by 38 nm whereas their entrapment efficiency was lowered by 1.3 % only. In vitro release studies revealed that 61.58 to 74.82 % of donepezil released from developed SLNs after 24 hours and found that drug release followed non-Fickian transport (0.556 to 0.658).

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Cite this article:
Manjunath Kopparam, Suresh V Kulkarni, Shivu SN. Development, Characterization and In vitro Evaluation of Donepezil solid Lipid Nanoparticles. Research J. Pharm. and Tech. 2020; 13(7): 3113-3121. doi: 10.5958/0974-360X.2020.00551.X

Cite(Electronic):
Manjunath Kopparam, Suresh V Kulkarni, Shivu SN. Development, Characterization and In vitro Evaluation of Donepezil solid Lipid Nanoparticles. Research J. Pharm. and Tech. 2020; 13(7): 3113-3121. doi: 10.5958/0974-360X.2020.00551.X Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-7-11

REFERENCES:

1. Dong ZH, Chang SX, Kai JH, Chang HZ. The production and characteristics of solid lipid nanoparticles (SLNs). Biomaterials. 2003; 24: 1781–1785.

2. Rainer HM, Karsten M, Sven G. Solid lipid nanoparticles (SLN) for controlled drug delivery-a review of the state of the art. Eur J Pharm Biopharm. 2000; 50: 161-177.

3. Muller RH, Radtke M, Wissing SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Adv Drug Deliv Rev. 2002; 54 Suppl. 1: S131–S155.

4. Sweetha G, Sangeetha B, Prabhu S. A review on curcumin nanoparticles and its controlled delivery to treat degenerative diseases. Asian J Pharm Tech. 2013; 3(4): 218-222.

5. Pramod S, Suvarna P, Nikhil B. Formulation and Evaluation of Solid Lipid Nanoparticle Based Transdermal Drug Delivery System for Alzheimer’s Disease. Res J Pharma Dosage Forms and Tech. 2016; 8(2):73-80.

6. Wolfgang M, Karsten M. Solid lipid nanoparticles Production, characterization and applications. Adv Drug Deliv Rev. 2001; 47: 165–196.

7. Vinay K, Anurag Kumar V, Dhyan Chandra Y. A review on solid lipid nanoparticles (SLNs). Research J Pharm and Tech. 2019; 12(11): 5605-5613.

8. Mayank S, Kamla P. Solid lipid nanoparticles of simvastatin: Pharmacokinetic and biodistribution studies on Swiss albino mice. Res J Pharma Dosage Forms and Tech. 2012; 4(6): 336-342.

9. Trilochan S, Prasanna Kumar P. Solid lipid nanoparticles: A novel carrier in drug delivery system. Res J Pharma Dosage Forms and Tech. 2013, 5(2): 56-61.

10. Emilio M, Roberta C, Otto C, Lorenzo R, Gasco MR. Scale-up of the preparation process of solid lipid nanospheres. Part I. Int J Pharm. 2000; 205: 3–13.

11. Jameel AM, Sarasija S Imtiyaz AK. Formulation, characterization and in vitro evaluation of methotrexate solid lipid nanoparticles. Research J Pharm and Tech. 2009; 2(4): 685-689.

12. Srikar G, Prameela Rani A, Raghuveer P. Voriconazole solid lipid nanoparticles: Optimization of formulation and process parameters. Research J Pharm and Tech. 2018; 11(7): 2829-2835.

13. Borkar S, Shende V, Chatap V, Sawant V, Suresh R, Ganesh D. Tamoxifen citrate loaded solid lipid nanoparticles- A novel approach in the treatment of ER+ breast cancer. Res J Pharma Dosage Forms and Tech. 2009; 1(2): 143-149.

14. David QG, David TE, Adriana GQ, Eric A, Eric D. Adaptation and optimization of the emulsification-diffusion technique to prepare lipidic nanospheres. Eur J Pharm Sci. 2005; 26: 211–218.

15. Prashant SW, Kshirsagar MD. Compatibility study in vitro drug release study of solid lipid nanoparticle based transdermal drug delivery system for rasagiline mesylate. Asian J Res Pharm Sci. 2017; 7(2): 92-96.

16. Schubert MA, Muller CC. Solvent injection as a new approach for manufacturing lipid nanoparticles – evaluation of the method and process parameters. Eur J Pharm Biopharm. 2003; 55: 125–131.

17. Fu QH, Sai PJ, Yong ZD, Hong Y, Yi QY, Su Z. Preparation and characterization of stearic acid nanostructured lipid carriers by solvent diffusion method in an aqueous system. Colloids and Surfaces B: Biointerfaces. 2005; 45: 167–173.

18. Krishna Veni D, Vishal Gupta N. Quality by design approach in the development of solid lipid nanoparticles of linagliptin. Research J Pharm and Tech. 2019; 12(9):4454-4462.

19. Buchake VV, Muthal AP, Saudagar RB, Bachhav RS. A neurodegenerative disorder-Alzheimer disease: A treatise. Res J Pharmacology and Pharmacodynamics. 2010; 2(4): 268-273.

20. Rajesh Kumar D, Siva Shankar M, Prathap Reddy P, Ram Sarath Kumar B, Sumalatha N. A review on Alzheimer’s disease. Res J Pharmacology and Pharmacodynamics. 2014; 6(1): 59-63.

21. Rahul PJ, Manohar DK, Omkar VN, Vikranti WK, Suraj BK. A review on Alzheimer’s Disease (AD) and its herbal treatment of Alzheimer’s disease. Asian J Res Pharm Sci 2019; 9(2):112-122.

22. Vivek Kumar S. Current therapeutic strategies for Alzheimer’s disease: A lost direction or a hope remains? Res J Pharmacology and Pharmacodynamics. 2010; 2(3): 215-220.

23. Mahalingam V, Vijayabaskar S, Kalaivani RA, Somanathan T. Analytical method development and validation for the analysis of donepezil hydrochloride and its related substances using ultra performance liquid chromatography. Research J Pharm and Tech. 2017; 10(8): 2743-2749.

24. Mohd Y, Udai Vir SS, Iti C, Praveen Kumar G, Alok Pratap S, Dinesh P, Ameeduzzafar. Solid lipid nanoparticles for nose to brain delivery of donepezil: formulation, optimization by Box–Behnken design, in vitro and in vivo evaluation. Artif Cells Nanomed Biotechnol. 2018; 46(8): 1838-1851.

25. Svapnil S, Misam P, Dipil P, Ruchita S2, Jayvadan P, Manish P. Nanoparticle Drug Delivery to Brain – A Review. Research J Pharm and Tech. 2012; 5(1):8-13.

26. Vobalaboina V, Kopparam M. Preparation, characterization and in vitro release kinetics of clozapine solid lipid nanoparticles. J Controlled Release. 2004; 95: 627– 638.

27. Jenning V, Schafer-Korting M, Gohla S. Vitamin A-loaded solid lipid nanoparticles for topical use: drug release properties. J Controlled Release. 2000; 66: 115–126.

28. Baviskar A, Hiremath S, Akul M and Pawar P. Effect of lipids and surfactants on solid lipid nanoparticle engineering. Research J Pharm and Tech. 2011; 4(4): 521-526.

29. Mohd Y, Iti C, Misbahu JH, Abdurazak JT, Prasoon KS. Formulation and evaluation of glyceryl behenate based solid lipid nanoparticles for the delivery of donepezil to brain through nasal route. Research J Pharm and Tech. 2018; 11(7): 2836-2844.

30. Kanchan RP, Sarika VK. A review on novel drug delivery system: A recent trend. Asian J Pharm Tech. 2019; 9(2):135-140.

31. Madgulkar AR, Bhalekar MR, Kapse SB, Paygude B, Reddi SS. Transdermal permeation enhancement of valsartan using solid lipid nanoparticles. Research J Pharm and Tech. 2011;4(8): 1297-1302.

32. Mitul RV, Nisha P, Divyakant P, Rajesh KS, Lalit Lata J. Mucoadhesive - nanoparticulate system for ocular delivery of loteprednol etabonate. Asian J Pharm Res. 2014; 4(2): 78-83.

33. Sachin J, Vishal Gupta N. Solid lipid nanoparticles – preparation, applications, characterization, uses in various cancer therapies: A review. Research J Pharm and Tech. 2013; 6(8): 825-837.

34. Savita M, Sarika L, Vijay R. Review on solid lipid nanoparticles for ophthalmic delivery. Res J Pharma Dosage Forms and Tech. 2019; 11(1): 19-34.

35. Yesha KP, Noolvi MN, Hasumati R, Meghna PP. Development and validation of stability indicating reverse phase high performance liquid chromatographic method for estimation of donepezil HCl from bulk drug. Asian J Pharm Res. 2015; 5(2): 96-101.

36. Ravi Kumar V, Hemanth Kumar G, Raveendra Babu G, Srinivasarao J. New comprehensive HPLC assay method for donepezil hydrochloride. Asian J Research Chem. 2011; 4(10): 1508-1512.

37. Thulasi Ram D, Subhashis D, Niranjan Babu M, Chakradhar Nath T, Thejeswi B. A Review on solid lipid nanoparticles. Research J Pharm and Tech. 2012; 5(11): 1359-1368.