Author(s): Rangisetty Spandana Yasaswini, Mukthinuthalapati Mathrusri Annapurna, Avuthu Sai Sheela

Email(s): mmukthin@gitam.edu

DOI: 10.5958/0974-360X.2020.00514.4   

Address: Rangisetty Spandana Yasaswini, Mukthinuthalapati Mathrusri Annapurna*, Avuthu Sai Sheela
Department of Pharmaceutical Analysis, GITAM Institute of Pharmacy, Gandhi Institute of Technology and Management, Visakhapatnam, Andhra Pradesh-530045, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 6,     Year - 2020


ABSTRACT:
Trifluridine is an anti-viral drug. A new stability indicating ultrafast liquid chromatographic method has been developed for the assay of Trifluridine in ophthalmic solutions and validated. Shimadzu Model CBM-20A/20 Alite UFLC system (Shimadzu Co., Kyoto, Japan) equipped with SPD M20A prominence photodiode array detector with C18 Agilent column (250 mm × 4.60 mm i.d. 5µm particle size) and a mobile phase consisting of Acetonitrile: water (50:50, v/v) with flow rate 0.8 ml/min (UV detection at 261 nm) was used for the present study. Trifluridine has shown linearity1-100 µg/mL with linear regression equation y = 66167x + 13368 (R² = 0.9999). Trifluridine was subjected to forced degradation and method was validated as per ICH guidelines.


Cite this article:
Rangisetty Spandana Yasaswini, Mukthinuthalapati Mathrusri Annapurna, Avuthu Sai Sheela. New stability indicating RP-UFLC method for the determination of Trifluridine – A potent antiviral drug. Research J. Pharm. and Tech 2020; 13(6): 2881-2885. doi: 10.5958/0974-360X.2020.00514.4

Cite(Electronic):
Rangisetty Spandana Yasaswini, Mukthinuthalapati Mathrusri Annapurna, Avuthu Sai Sheela. New stability indicating RP-UFLC method for the determination of Trifluridine – A potent antiviral drug. Research J. Pharm. and Tech 2020; 13(6): 2881-2885. doi: 10.5958/0974-360X.2020.00514.4   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-6-64


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