Author(s):
Avuthu Sai Sheela, Mukthinuthalapati Mathrusri Annapurna, Rangisetty Spandana Yasaswini
Email(s):
mmukthin@gitam.edu
DOI:
10.5958/0974-360X.2020.00502.8 
Address:
Avuthu Sai Sheela, Mukthinuthalapati Mathrusri Annapurna*, Rangisetty Spandana Yasaswini
Department of Pharmaceutical Analysis, GITAM Institute of Pharmacy, GITAM (Deemed to be) University, Visakhapatnam-530045, India.
*Corresponding Author
Published In:
Volume - 13,
Issue - 6,
Year - 2020
ABSTRACT:
Budesonide is a glucocorticoid used in the long-term management of asthma and chronic obstructive pulmonary disease (COPD). Also used to prevent difficulty breathing, chest tightness, wheezing and coughing caused by asthma. A new stability indicating RP-UFLC method has been developed and validated for the determination of Budesonide in pure and pharmaceutical dosage forms. Shimadzu Model CBM-20A/20 Alite UFLC system (Shimadzu Co., Kyoto, Japan) equipped with SPD M20A prominence photodiode array detector on Agilent C18 column was used with a mobile phase consisting of formic acid and ACN (25: 75, v/v) and flow rate 1.2 ml/min (UV detection at 247 nm). Budesonide obeys Beer-Lambert’s law over a concentration range of 10-100 µg/ml. The linear regression equation was found to be y = 35317x + 3515.8 (r2 = 0.9999). The LOD and LOQ was found to be and 2.913 and 8.7643 µg/ml. Budesonide was subjected to forced degradation and the method was validated as per ICH guidelines.
Cite this article:
Avuthu Sai Sheela, Mukthinuthalapati Mathrusri Annapurna, Rangisetty Spandana Yasaswini. New validated stability indicating RP-UFLC method for the determination of Budesonide. Research J. Pharm. and Tech 2020; 13(6): 2819-2824. doi: 10.5958/0974-360X.2020.00502.8
Cite(Electronic):
Avuthu Sai Sheela, Mukthinuthalapati Mathrusri Annapurna, Rangisetty Spandana Yasaswini. New validated stability indicating RP-UFLC method for the determination of Budesonide. Research J. Pharm. and Tech 2020; 13(6): 2819-2824. doi: 10.5958/0974-360X.2020.00502.8 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-6-52
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