Tekeshwar Kumar, Amber Vyas, Vishal Jain
email@example.com , firstname.lastname@example.org
Tekeshwar Kumar1, Amber Vyas2, Vishal Jain2*
1M J College, Kohka, Junwani Road,Bhilai, Durg, Chhattisgarh, 491 023, India.
2University Institute of Pharmacy, Pt. Ravishankar University, Raipur, Chhattisgarh, 492 010, India.
Volume - 13,
Issue - 6,
Year - 2020
WHO is highly recommended the customary exploitation of traditional therapeutics of medicinal plants and is consequently in pipeline for development of monograph and exploring their uncultivated pharmacological properties. Presently, correct identification of medicinal plants by phytochemical fingerprints is a speedy tool to ensure reproducible quality of herbal drugs. In our study, the ethyl acetate and methanol extract of leaves of Lannea coromandelica (L. coromandelica) was subjected for chemical fingerprint as well therapeutic aid as antifilarial agents. High performance thin layer chromatography (HPTLC) was employed to develop chemical fingerprint and filariciadal activity was assessed by motility inhibition and MTT reduction assay with concentrations range 1000 to 25µg/ml. The HPTLC analysis of ethyl acetate and methanol extract was carried out using hexane: ethyl acetate: formic acid: methanol (5:4:1:0.5 v/v/v/v) for fingerprinting and quantification ellagic acid and quercetin. The HPTLC method was found to give compact spots for Rf = 0.54 and 0.51 ellagic acid; Rf = 0.91 and 0.87 quercetin for ethyl acetate and methanol extract respectively. The HPTLC method was validated as per the ICH guidelines. Inhibitory concentration (IC50) for the ethyl acetate extract was found to be 284.5µg/ml. In motility assay, complete inhibition of motility was observed for all concentrations. Hence, our study could be valuable for inventing strategies for quality control parameter and justifies the ethnic uses of plant in folkloric medicines.
Cite this article:
Tekeshwar Kumar, Amber Vyas, Vishal Jain. Phytochemical and pharmacological screening of leaf of Lannea coromandelica Linn. Research J. Pharm. and Tech 2020; 13(6): 2815-2818. doi: 10.5958/0974-360X.2020.00501.6
1. Sahare KN, Singh V. Antifilarial activity of ethyl acetate extract of Vitex negundoin vitro. Asian Pacific Journal of Tropical Medicine. 2013; 6(9): 689-692.
2. Tewari S, Chauhan PM, Bhaduri AP, Fatima N, Chatterjee, RK. Synthesis and antifilarial profile of 7-chloro-4-(substituted amino) quinolines: a new class of antifilarial agents. Bioorganic & Medicinal Chemistry Letters. 2000; 10: 1409-1412.
3. Kumar T., Sahu A., Jain V. In vitro screening of lannea coromandelica for antifilarial activity on cattle filarial parasite setaria cervi. Indian drugs. 2014; 51(8); 28-34.
4. Chopra RN. 1956, Glossary of Indian Medicinal Plants. CSIR. Publications, New Delhi, India.
5. Kumar T., Sahu A., Jain V. Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays. Tekeshwar Kumar, Vishal Jain. Scientiﬁca. Volume 2015, Article ID 203679, 13 pages, http://dx.doi.org/10.1155/2015/203679.
6. Yoshiake A, Okada M, Tsuji S, Tonogai Y. High performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits. Journal of Chromatography A. 2000: 896: 87-93.
7. International Conference on Harmonization (ICH), Validation of Analytical Procedures: Text and Methodology Q2 (R1), 2005.
8. International Conference on Harmonization (ICH), Validation of Analytical Procedures: Text on Validation of Analytical Procedures Q2A, 1994.
9. Murthy PK. Evaluation of two in vitro systems employing Brugia malayi parasite for prescreening of potential antifilarials. Current Scienc.1999; 77(8): 1084-1089.
10. Strote G, Bonow I, Kromer M, de Kromer TR, Attah S, Opoku N. Chemotherapy for onchocerciasis: results of in vitro experiments with promising new compounds. Tropical Medicine & International Health. 1998; 3: 397-407.