Author(s): Saikhu Akhmad Husen, Muhamad Frendy Setyawan, Muhammad Farraz Syadzha, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Arif Nur Muhammad Ansori, Mochammad Amin Alamsjah, Zulfa Nailul Ilmi, Pugar Arga Cristina Wulandari, Pratiwi Pudjiastuti, Khalijah Awang, Dwi Winarni

Email(s): dwi-w@fst.unair.ac.id

DOI: 10.5958/0974-360X.2020.00491.6   

Address: Saikhu Akhmad Husen1,2, Muhamad Frendy Setyawan1, Muhammad Farraz Syadzha1, Raden Joko Kuncoroningrat Susilo1, Suhailah Hayaza1, Arif Nur Muhammad Ansori3, Mochammad Amin Alamsjah4, Zulfa Nailul Ilmi5, Pugar Arga Cristina Wulandari5, Pratiwi Pudjiastuti5, Khalijah Awang6, Dwi Winarni1,2*
1Department of Biology, Faculty of Science and Technology,Universitas Airlangga, 60115, Surabaya, Indonesia.
2Animal Histology Laboratory, Faculty of Science and Technology, Universitas Airlangga, 60115, Surabaya, Indonesia.
3Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, 60115, Surabaya, Indonesia.
4Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, 60115, Surabaya, Indonesia.
5Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, Indonesia.
6Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia.
*Corresponding Author

Published In:   Volume - 13,      Issue - 6,     Year - 2020


ABSTRACT:
This study aims to evaluate the effects of alginate extract from Sargassum ilicifolium and okra (Abelmoschus esculentus) pods extract on the re-epithelialization rate of the wound area and the number of neutrophils, macrophages, fibroblasts, fibrocytes, and collagen density in streptozotocin-induced diabetic mice. This study was done in vivo using male mice (strain BALB/C, 3 to 4 months old, 30 to 40g). The normal control group (KN) and the diabetic groups (induced by streptozotocin). The diabetic group was divided into 9 groups: three diabetic control groups (KD), three alginate treatment groups (KAI), and three treatment groups of alginate-okra (Abelmoschus esculentus) pods extract (KAIO). The treatment dose was 50 mg/kg body weight. Measurement of fasting blood sugar levels was done before and after streptozotocin injection. The treatment was carried out in three different time periods: 3 days, 7 days, and 14 days. Then, mice skin was cut and proceed for histopathological analysis. Interestingly, we indicated that the administration of okra (Abelmoschus esculentus) pods extract combined with alginate from Sargassum ilicifolium could increase the rate of re-epithelialization of the wound area, increase the number of neutrophils, macrophages, fibroblasts, fibrocytes, as well as increase synthesized collagen. The combined extract was able to improve the healing process of open wounds in diabetic mice. Therefore, it can be concluded that the administration of alginate from Sargassum ilicifolium and okra (Abelmoschus esculentus) pods extract was able to improve open wounds healing in diabetic mice due to the role of antioxidant from polysaccharides and quercetin which acted as anti-hyperglycemic to anti-inflammatory properties.


Cite this article:
Saikhu Akhmad Husen, Muhamad Frendy Setyawan, Muhammad Farraz Syadzha, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Arif Nur Muhammad Ansori, Mochammad Amin Alamsjah, Zulfa Nailul Ilmi, Pugar Arga Cristina Wulandari, Pratiwi Pudjiastuti, Khalijah Awang, Dwi Winarni. A Novel Therapeutic effects of Sargassum ilicifolium Alginate and Okra (Abelmoschus esculentus) Pods extracts on Open wound healing process in Diabetic Mice. Research J. Pharm. and Tech 2020; 13(6): 2764-2770. doi: 10.5958/0974-360X.2020.00491.6


REFERENCES:
1.    Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Research and Clinical Practice. 2010; 87(1): 4-14.
2.    Husen  SA, Wahyuningsih SPA, Ansori ANM, Hayaza S, Susilo RJK, Winarni D, Punnapayak  H, Darmanto W. Therapeutic effect of okra (Abelmoschus esculentus Moench) pods extract on streptozotocin-induced type-2 diabetic mice. Research Journal of Pharmacy and Technology. 2019; 12(8): 3703-3708.
3.    Novelli M, Bonamassa B, Masini M, Funel N, Canistro D, De Tata V, Martano M, Soleti A, Campani D, Paolini M, Masiello P. Persistent correction of hyperglycemia in streptozotocin-nicotinamide-induced diabetic mice by a non-conventional radical scavenger. Naunyn-Schmiedeberg's Archives of Pharmacology. 2010; 382(2): 127-137.
4.    Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Husen SA. Renoprotection by Garcinia mangostana L. pericarp extract in streptozotocin-induced diabetic mice. Iraqi Journal of Veterinary Sciences. 2019; 33(1): 13-19.
5.    Husen SA, Wahyuningsih SPA, Ansori ANM, Hayaza S, Susilo RJK, Darmanto W, Winarni D. The effect of okra (Abelmoschus esculentus Moench) pods extract on malondialdehyde and cholesterol level in STZ-induced diabetic mice. Ecology, Environment and Conservation. 2019; 25: 51-55.
6.    Mendes JJ, Leandro CI, Bonaparte DP, Pinto AL. A rat model of diabetic wound infection for the evaluation of topical antimicrobial therapies. Comparative Medicine. 2012; 62(1): 37-48.
7.    Husen SA, Winarni D, Khaleyla F, Kalqutny SH, Ansori ANM. Activity assay of mangosteen (Garcinia mangostana L.) pericarp extract for decreasing fasting blood cholesterol level and lipid peroxidation in type-2 diabetic mice. AIP Conference Proceedings. 2017; 1888(1): 020026-1-6.
8.    Husen SA, Khaleyla F, Kalqutny SH, Ansori ANM, Susilo RJK, Alymahdy AD, Winarni D. Antioxidant and antidiabetic activity of Garcinia mangostana L. pericarp extract in streptozotocin-induced diabetic mice. Bioscience Research. 2017; 14(4): 1238-1245.
9.    Mohora M, Vîrgolici B, Paveliu F, Lixandru D, Muscurel C, Greabu M. Free radical activity in obese patients with type 2 diabetes mellitus. Romanian Journal Internal Medicine. 2006; 44(1): 69-78.
10.    Husen SA, Salamun, Khaleyla F, Ansori ANM, Susilo RJK, Winarni D. Antioxidant activity assay of alpha-mangostin for amelioration of kidney structure and function in diabetic mice. Advances in Social Science, Education and Humanities Research (ASSEHR). 2018; 98: 84-88.
11.    Ferroni P, Basili S, Falco A, Davì G. Platelet activation in type 2 diabetes mellitus. Journal of Thrombosis Haemostasis. 2004; 2(8): 1282-1291.
12.    Brem H, Tomic-Canic M. Cellular and molecular basis of wound healing in diabetes. Journal of Clinical Investigation. 2007; 117(5): 1219-1222.
13.    Husen SA, Winarni D, Salamun, Ansori ANM, Susilo RJK, Hayaza S. Hepatoprotective effect of gamma-mangostin for amelioration of impaired liver structure and function in streptozotocin-induced diabetic mice. IOP Conference Series: Earth and Environmental Science. 2019; 217: 012031.
14.    Purnamasari R, Winarni D, Permanasari AA, Agustina E, Hayaza S, Darmanto W. Anticancer activity of methanol extract of Ficus carica leaves and fruits against proliferation, apoptosis, and necrosis in Huh7it cells. Cancer Informatics. 2019; 18: 1-7.
15.    Fadholly A, Ansori ANM, Jayanti S, Proboningrat A, Kusala MJK, Putri N, Rantam FA, Sudjarwo SA. Cytotoxic effect of Allium cepa L. extract on human colon cancer (WiDr) cells: in vitro study. Research Journal of Pharmacy and Technology. 2019; 12(7): 3483-3486.
16.    Adelakun OE, Oyelade OJ, Ade-Omowaye BIO, Adeyemi IA, Van de Venter M, Koekemoer TC. Influence of pre-treatment on yield chemical and antioxidant properties of a Nigerian okra seed (Abelmoschus esculentus Moench) flour. Food and Chemical Toxicology. 2009; 47(3): 657-661.
17.    Sengkhamparn N, Chanshotikul N, Assawajitpukdee C, Khamjae T. Effects of blanching and drying on fiber rich powder from pitaya (Hylocereus undatus) peel. International Food Research Journal. 2013; 20(4): 1595-1600.
18.    Akbarzadeh S, Gholampour H, Farzadinia P, Daneshi A, Ramavandi B, Moazzeni A, Keshavarz M, Bargahi A. Anti-diabetic effects of Sargassum oligocystum on streptozotocin-induced diabetic rat. Iran Journal of Basic Medical Science. 2018; 21(3): 342-346.
19.    Jo S-H, Ka E-H, Lee H-S, Apostolidis E, Jang H-D, Kwon Y-I. Comparison of antioxidant potential and rat intestinal α-glucosidases inhibitory activities of quercetin, rutin, and isoquercetin. International Journal of Applied Research in Natural Products. 2009; 2(4): 52-60.
20.    Murray M, Dordevic AL, Ryan L, Bonham MP. The impact of a single dose of a polyphenol-rich seaweed extract on postprandial glycaemic control in healthy adults: a randomised cross-over trial. Nutrients. 2018; 10(3): 270.
21.    Husni A, Subaryono, Pranoto Y, Tazwir, Ustadi. Development of alginate extraction method from Sargassum sp. as thickening. AGRITECH. 2012; 32(1): 1-8.
22.    Nugraha AS, Keller PA. Revealing indigenous Indonesian traditional medicine: anti-infective agents. Natural Product Communications. 2011; 6(12): 1953-1966.
23.    Wahyuni DK, Ansori ANM, Vidiyanti F. GC-MS analysis of phytocomponent in methanolic extracts of leaf-derived callus of Justicia gendarussa Burm.f. Bioscience Research. 2017; 14(3): 668-677.
24.    Ansori ANM, Kusala MJK, Irawan H, Putri N, Fadholly A, Proboningrat A, Rukmana S, Karni I, Anisa AK, Adrianto H. Citrus reticulata extract as biocides to control Aedes aegypti, the vector of dengue. Bioscience Research. 2018; 15(3): 1661-1665.
25.    Yende SR, Harle UN, Chaugule BB. Therapeutic potential and health benefits of Sargassum species. Pharmacognosy Review. 2014; 8(15): 1-7.
26.    Aderibigbe BA, Buyana B. Alginate in wound dressings. Pharmaceutics. 2018; 10(2): 42.
27.    Falanga V. Wound healing and its impairment in the diabetic foot. Lancet. 2005; 366(9498): 1736-1743.
28.    Maruyama K, Asai J, Ii M, Thorne T, Losordo DW, D'Amore PA. Decreased macrophage number and activation lead to reduced lymphatic vessel formation and contribute to impaired diabetic wound healing. American Journal of Pathology. 2007; 170(4): 1178-1191.
29.    Wahyuningsih SPA, Pramudya M, Putri IP, Winarni D, Savira NII, Darmanto W. Crude polysaccharides from okra pods (Abelmoschus esculentus) grown in Indonesia enhance the immune response due to bacterial infection. Advances in Pharmacological Sciences. 2018; 2018: 850538.
30.   Patel S, Srivastava S, Singh MR, Singh D. Mechanistic insight into diabetic wounds: pathogenesis, molecular targets and treatment strategies to pace wound healing. Biomedicine & Pharmacotherapy. 2019; 112: 108615.

Recomonded Articles:

Author(s): Nimbalkar V.V., Pansare P.M., Nishane B.B.

DOI: 10.5958/0974-360X.2015.00310.8         Access: Open Access Read More

Author(s): S. Subasree, Karthikeyan Murthykumar, Dhanraj

DOI: 10.5958/0974-360X.2016.00116.5         Access: Open Access Read More

Author(s): Raj Kumar R, Praveen D, Palani Shanmugasundaram

DOI: 10.5958/0974-360X.2016.00164.5         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): M. Sumithra, Avantika Prabhabanik

DOI: 10.5958/0974-360X.2017.00036.1         Access: Open Access Read More

Author(s): Deepak Karki, Gururaj S. Kulkarni, Shivakumar Swamy, Sheeba FR

DOI: 10.5958/0974-360X.2017.00750.8         Access: Open Access Read More

Author(s): Praveen D, Ranadheer Chowdary P, Gandikota Thanmayi, Gangabathina Poojitha, Vijey Aanandhi M

DOI: 0.5958/0974-360X.2016.00047.0         Access: Open Access Read More

Author(s): B. Thawkar, M. Kale, M. Oswal, K. Maniyar, K. Kadam, S. Kamat

DOI: 10.5958/0974-360X.2016.00044.5         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Priya P. Munshi, D.S. Mohale, R. Akkalwar, A.V. Chandewar

DOI: Not Available         Access: Open Access Read More

Author(s): Vaseeha Banu T.S., Sandhya K.V., K.N. Jayaveera

DOI: Not Available         Access: Open Access Read More

Author(s): Sandesh More, Javed Mirza, Nanasaheb Kale, Mayur Gandhi, Rakesh Chaudhari

DOI: Not Available         Access: Open Access Read More

Author(s): Barokar A.A., Wagh R.D., Baviskar D.T., Shaikh T.J.

DOI: Not Available         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags