Author(s):
Sivakumar G, Gopalasatheeskumar K, Gowtham K, Sindhu E, Akash Raj K, Rajaguru B, Sriram K, Kalaichelvan V K
Email(s):
gskpungai@gmail.com
DOI:
10.5958/0974-360X.2020.00490.4 
Address:
Sivakumar G1, Gopalasatheeskumar K2*, Gowtham K1, Sindhu E1, Akash Raj K1, Rajaguru B1, Sriram K2, Kalaichelvan V K2
1Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, Tamil Nadu, India (Affiliated to Tamil Nadu Dr. M.G.R. Medical University).
2Department of Pharmacy, Annamalai University, Chidambaram, Tamil Nadu, India.
*Corresponding Author
Published In:
Volume - 13,
Issue - 6,
Year - 2020
ABSTRACT:
Arthritis is one of the foremost health troubles worldwide, leading causes of disability in western and developing countries. Therapies developed along the principles of western medicine are often limited in efficacy, carry the risk of adverse effects, and are often too costly, especially for the developing world. Aegle marmelos (L.) Correa (AM) commonly known as Beal or Bilva belonging to the family Rutaceae has been widely used in indigenous systems of Indian medicine due to its various medicinal properties. The secondary metabolites (phytochemicals) present in medicinal plants relation for their therapeutic value. For example, triterpenoids, flavonoids and related polyphenols have antiarthritic properties. But since there is no report on antiarthritic activity of unriped fruit extract of AM. Therefore aim of the current study is to evaluate the antiarthritic activity of AM. Phytochemical analysis of different extracts of AM revealed the presence of various bioactive phytochemical compounds was found in aqueous, methanolic, ethylacetate, chloroform and petroleum ether extracts of AM. In vitro anti-arthritic activity of different extracts of AM was screened against protein denaturation against bovine serum albumin and egg albumin. The fruit extract of AM showed significantly higher anti-arthritic activity at increasing concentration. The flavonoids and triterpenoids present in AM may be the reason for this anti-arthritic activity. Hence, AM can be used as an anti-arthritic agent. The investigation is based on the need for anti-inflammatory/ antiarthritic agents from natural sources with potent activity and lesser side effects as substitutes for chemical therapeutics. In future it would be important to understand molecular mechanism of Aegle marmelos.
Cite this article:
Sivakumar G, Gopalasatheeskumar K, Gowtham K, Sindhu E, Akash Raj K, Rajaguru B, Sriram K, Kalaichelvan V K. Phytochemical analysis, Antioxidant and Antiarthritic activities of different solvent extract of Aegle marmelos L. unripe fruit. Research J. Pharm. and Tech 2020; 13(6): 2759-2763. doi: 10.5958/0974-360X.2020.00490.4
Cite(Electronic):
Sivakumar G, Gopalasatheeskumar K, Gowtham K, Sindhu E, Akash Raj K, Rajaguru B, Sriram K, Kalaichelvan V K. Phytochemical analysis, Antioxidant and Antiarthritic activities of different solvent extract of Aegle marmelos L. unripe fruit. Research J. Pharm. and Tech 2020; 13(6): 2759-2763. doi: 10.5958/0974-360X.2020.00490.4 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-6-40
REFERENCES:
1. Black PH, Garbutt GD. Stress, inflammations and cardiovascular disease. Journal of psychosomatic research. 2002; 52(1):1-23.
2. Dhankhar S. Aegle marmelos (Linn.) Correa: A source of Phytomedicine. Journal of Medicinal Plants Research. 2010; 5(9):1497-1507.
3. Sukhdev AR. A selection of prime ayurvedic plant drugs – Ancient modern concordance. Anamaya publication. 2003; 55-58.
4. Robbers JE, Tyler VE. Herbs of choice – The therapeutic use of phytomedicines. International journal of pharma sciences. 2002; 3(2):199-203.
5. Gopalasatheeskumar K, Ariharasivakumar G, Sengottuvel T et al. Confirmation of Best Method for Detection of Alpha Amylase Activity of Cucumis Melo Var Agrestis (Wild Musk Melon). J Adv Res BioChem Pharma. 2019; 2(1):1-3.
6. Gopalasatheeskumar K. Significant Role of Soxhlet Extraction Process In Phytochemical Research. Mintage Journal of Pharmaceutical & Medical Sciences. 2018; 7(1):43-47.
7. Gopalasatheeskumar K, Parthiban S, Manimaran T, Boopathi T. Phytochemical Screening on Various Extracts (Benzene, Ethanolic and Aqueous) of Stem Parts of Zanthoxylum rhetsa (Roxb.) Dc. IJUPBS. 2017; 6(2):79-91.
8. Karthishwa K, Mirunalini S, Dhamodhara G, Krishnaven M, Arulmozhi V. Phytochemical Investigation of Methanolic Extract of the Leaves of Pergulariadaemia. J Biol Sci. 2010; 10(3):242-246.
9. Parthiban S, Gopalasatheeskumar K, Boopathi T, Sangeetha G, Thanga Kokila M, Sanish Devan V. In Vitro Anti-Inflammatory Activity of Stem of Zanthoxylum Rhetsa (Roxb.) Dc. World Journal of Pharmaceutical Research. 2017; 6(3): 591-600.
10. Boopathi T, Gopalasatheeskumar K, Parthiban S, Sangeetha G, Thanga Kokila M, Manimaran T. Evaluation of Antimicrobial Activity of Tecoma Stans and Muntingia Calabura. World Journal of Pharmaceutical Research. 2007; 6(3): 617-627.
11. Trease GE, Evans WC. Textbook of Pharmacognosy. 12th edn. BallieseTindall and Company Publisher, London.1983 pp. 343-383.
12. Sridevi G, Srividya S, Sembulingam K, Sembulingam P. An evaluation of in vitro and in vivo free radical scavenging and antioxidant potential of ethanolic extract of Pergularia daemia. Biocatalysis and Agricultural Biotechnology. 2018; 15: 131-137.
13. Srividhya V, Sengottuvel Thangavel, Gopalasatheeskumar K, Kanupriya J, Arihara Sivakumar G. Antioxidant potential and Phytochemical analysis of fruit extract of Cucurbita pepo. Int. J. Curr. Res. Chem. Pharm. Sci. 2019; 6(3): 22-32.
14. Chandran R, Parimelazhagan T, George B. Antihyperglycemic activity of the bark methanolic extract of Syzygium mundagam in diabetic rats. Alexandria Journal of Medicine. 2017; 53(4): 317-324.
15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice RE. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 1999; 26:1231–1237.
16. Lavanya R, Maheshwari US, Harish G, Raj B, Kamali SJ, Hemamalani D, Bharathvarma J, Umamaheswara Reddy. Investigation of in vitro anti-inflammatory, anti-platelet and anti-arthritic activities in the leaves of Anisomeles malabarica Linn., Res. J. Pharm. Biol. Chem. Sci., 2010; 1: 745-749.
17. Pavithra TK, Smitha KP, Kulashekar KS and Ashok Kumar BS. Evaluation of in vitro Anti-Arthritic Activity of Vitexnegundo against the Denaturation of Protein. Int.J.Curr.Microbiol.App.Sci. 2015; 4 (9): 87-90.
18. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J. Pharm. Pharmacol. 1968; 20 (3): 169-173.
19. Rahman H, Eswaraiah MC, Dutta AM. In-vitro anti-inflammatory and anti-arthritic activity of Oryza sativa var. joha rice (an aromatic indigenous rice of Assam). American-Eurasian J Agric Environ Sci. 2015; 15:115-121.
20. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm. 1968; 20:169-173.
21. Elisha IL, Dzoyem JP, McGaw LJ, Botha FS, Eloff JN. The anti-arthritic, anti-inflammatory, antioxidant activity and relationships with total phenolics and total flavonoids of nine South African plants used traditionally to treat arthritis. BMC Complement Altern Med. 2016; 23(16) :307.