Ruth Christiya C, Radhika J
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Ruth Christiya C1*, Radhika J2
1Research Scholar, PG and Research Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), Tiruchirapalli - 620005, Tamil Nadu, India.
2Associate Professor and Principal, PG and Research Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), Tiruchirapalli-620005, Tamil Nadu, India.
Volume - 13,
Issue - 6,
Year - 2020
Objective: To explore the physicochemical properties and free radical scavenging activity of Delonix elata L Leaf extract. Methods: The physical evaluation was carried out for the determination of physicochemical profile such as ash values (total ash, water soluble ash and acid insoluble ash), moisture content, various extractive yields and fluorescence analysis of Delonix elata L. The in-vitro antioxidant activities of leaf extract were evaluated by DPPH, Reducing power, Nitric oxide and ABTS+ assay. Results: Physicochemical analysis shows the quality and purity of the crude extract. More water-soluble ash content was found to be higher denoting the enhanced water solubility. Water extractive value 10.05% indicates that water permeates the cells of the leaves and thus, a better extractive value was obtained compared to ethanol, ethyl acetate, hexane and chloroform extract. Fluorescence analysis of leaf extracts of Delonix elata L showed characteristic coloration with various chemicals. In antioxidant studies, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals. The aqueous extract was also shown to have high phenolic and flavonoid content i.e. 39.49 ± 0.07 and 21.08 ± 0.10 mg/g equivalent than ethanol extract. This may be attributed to the antioxidant potential of leaf against free radical mediated diseases. Conclusion: This study suggests that the Delonix elata L leaf extract contained pharmacologically active compounds that validating the ethno pharmacological claims. This supports its traditional use for the improvement and maintenance of human health.
Cite this article:
Ruth Christiya C, Radhika J. Screening of Physicochemical properties and In Vitro free radical scavenging activity of Delonix elata L. leaf extracts. Research J. Pharm. and Tech 2020; 13(6): 2727-2732. doi: 10.5958/0974-360X.2020.00485.0
Ruth Christiya C, Radhika J. Screening of Physicochemical properties and In Vitro free radical scavenging activity of Delonix elata L. leaf extracts. Research J. Pharm. and Tech 2020; 13(6): 2727-2732. doi: 10.5958/0974-360X.2020.00485.0 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-6-35
1. Huang D, Chen HJ, Lin CD, Lin YH. Antioxidant and antiproliferative activities of water spinach (Ipomea aquatica Frosk.) constituents. Bot Bull Acad Sin. 2005:406; 99-106
2. Halliwell B. Antioxidant characterization. Biochem Pharmacol. 1995: 49; 1341-1348.
3. Gladis Raja Malar C, Chellaram C. Studies on Phytochemical screening, antioxidant activity and anti-bacterial activity of Salacia oblonga stem extract. International Journal of Pharmacy and Pharmaceutical Sciences. 2016: 8(1) ;32-36
4. Hyoung Lee S. Antioxidant activity of browing reaction products isolated from storage aged orange juice. J Agric Food Chem. 1992: 40; 550-552.
5. Ghada Abd El MH. Invitro studies on Delonix elata L an endangered medicinal plant. World Applied Sci J .2011: 14(5); 679-686
6. Murugananthan G, Mohan S. Anti- Inflammatory and Anti-arthritic activities of Delonix elata bark Extracts. Int J of Res in Ayur and Pharm. 2011: 2(6); 1819-1821
7. Renato B, Luciana GW, Gisely CL, Joao CPD. Quality control and TLC profile data on selected plants species commonly found in the Brazilian market. Rev Bras Farmacogn Braz J Pharmacognosy. 2012: 22; 1111-1118.
8. Sutar N, Garai R, Sharma US, Sharma UK. Anthelmintic activity of Platycladus orientalis leaves extract. International Journal of Parasitology Research. 2010: 2(2); 1-3.
9. The Ayurvedic Pharmacopoeia of India Part-II (formulations). The controller of publication, Govt of India, Ministry of Health and Family welfare, New Delhi. 2007.
10. Chase CR and Pratt RJ. Fluorescence of powdered vegetable drugs with particular reference to development of a system of identification. J Am pharm Ass. 1949: 38;324-333
11. Chang C, Yang M, Wen Chem H. Estimation of flavonoid total content in propolis by two complementary colorimetric methods. 2002: 10; 178-182
12. Amin I, Zamaliah MM, Chin WF. Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry. 2004: 87(4); 581-586.
13. Gyamfi MA, Yonamine M and Aniya Y. Free radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally induced liver injuries. Gen Pharmacol. 2002: 32; 661-667
14. Manmohan Singhal A, Paul HP and Singh. Synthesis and reducing power assay of methyl 601 semicarbazone derivatives. J Saudi Chem Soc. 2011:18;121–127
15. Sreejayan N and Rao MN. Free radical scavenging activity by Curcummoids. J Pharm Pharmacology. 1997: 47; 105.
16. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice Evans C. Antioxidant activity applying an improved ABTS radical cation decolourization free radical assay. Biol Med. 1999:26;1231-1237
17. Rajesh P and Latha S. “Capparis sepiaria Linn. – Pharmacognostical standardization and toxicity profile with chemical compounds identification (GC-MS)”. Int. J. Phytomed. 2010: 2(1);71-79
18. Patnia S and Saha AN. “Physicochemical, phytochemical and elemental analysis of stem bark and roots of Berberisasiatica”. Adv in App Sci Res. 2012: 3(6); 3624- 3628.
19. Purohit AP, Kokate CK and Gokhale SB. Pharmacognosy 13th Edition, Nirali prakashan India. 2005:256 – 259
20. Anonymous. Food and Nutrition Board. Dietary reference intakes for water, potassium, sodium, chloride, and sulfate. The National Academies Press. 2005: 77-185.
21. Labiad MH, Harhar H, GhanimiA, Tabyaoui M. Phytochemical Screening and Antioxidant Activity of Moroccan Thymus satureioïdes extracts. Journal of Materials and Environmental Sciences. 2017: 8(6); 2132-2139
22. Ranjith D. Fluorescence analysis and extractive values of herbal formulations used for wound healing activity in animals. Journal of Medicinal Plants Studies. 2018: 6(2); 189-192
23. Deepa VS, Kumar PS, Latha S, Selvamani P, srinivasan S. Medicinal plants and traditional medicine in Africa. Africa J Biotech. 2009: 8(8); 1630-1636.
24. Atanassova S, Georgieva K, Ivancheva. Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of the University of Chemical Technology and Metallurgy. 2011: 46(1); 81-88
25. Battu GR, Ethadi SR, Veda Priya G, Swathi Priya K, Chandrika K, Venkateswara Rao A and Satya OR. Evaluation of antioxidant and anti-inflammatory activity of Euphorbia heyneana Spreng. Asian Pacific Journal of Tropical Biomedicine. 2011: 191-194.
26. Parekh J, Darsharna J and Chanda S. Efficacy of aqueous and methanol extracts of some medicinal plants for potential antimicrobial activity. Turkish Journal of Biology. 2007:29;203-210
27. Armijos CP, Meneses MA, Guamán-Balcázar MC, Cuenca M and Suarez AI. Antioxidant properties of medicinal plants used in the Southern Ecuador. Journal of Pharmacognosy and Phytochemistry. 2018: 7(1); 2803-2812
28. Surya S, Poornima K, Ravikumar G, Kalaiselvi M, Gomathi D, Gopalakrishnan VK and Uma C. In-vitro antioxidant activity and phytochemical screening of ethanolic extract of Tabernaemontana coronaria (L.) Pharmacologyonline. 2018: 2; 212-218.
29. Karuna DS, Dey Kundu A, Vishal V, Bhakta T. Evaluation of In vitro Antioxidant Potential of Aconitum napellus Linn. Root Extract. International Journal of Pharmacognosy and Chinese Medicine. 2018: 2(2); 125- 129.
30. Ganesh Chandra Jagetia, Shaival Kamalaksh Rao, Manjeshwar Shrinath Baliga and Kiran Babu S. The Evaluation of Nitric Oxide Scavenging Activity of Certain Herbal formulations in vitro: A Preliminary Study. Phytother Res. 2004: 18; 561–565.
31. Zanwar AA, Hegde MH and Bodhankar SL. Cardioprotective activity of flax lignin concentrate extracted from seeds of L. usitatissimum in isoprenalin induced myocardial necrosis in rats. Interdisciplinary Toxicology. 2011: 4; 90-97.
32. Ho YL, Huang SS, Deng JS, Lin YH, Chang YS and Huang GH. In vitro antioxidant properties and total phenolic contents of wetland medicinal plants in Taiwan. Botanical Studies. 2012: 53; 55-66.
33. Krishnamoorthy M, Sasikumar JM, Shamna R, Pandiarajan C, Sofia P and Nagarajan B. Antioxidant activities of bark extract from mangroves, Bruguiera cylindrical L Blime and Ceriops decandra Per. Indian Journal Pharmacology. 2011: 43(5); 557-562.
34. Sangeetha S, Marimuthu P, Doraisamy P and Sarada DVL. Evaluation of antioxidant activity of the antimicrobial fraction from Sphaeranthus indicus. International Journal of Applied Biology and Pharmaceutical Technology. 2010: 1(2); 431-436.
35. Amarowicz R, Peg RB, Rahimi mohaddam P, Barl B and Weil JA. Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairie. Food Chemistry. 2004: 84; 551-562.