ABSTRACT:
The rapid, accurate and sensitive HPTLC method was used for quantification of flavonoids in Syzygium cumini (Linn.), a medicinally important plant of family Myrtaceae. The present analysis was done using five different solvents, ethanol, methanol, ethyl acetate, chloroform and water. The leaf extraction was done using sonication method. HPTLC analysis of leaf extracts in S. cumini responded well with good quantified amount of targeted flavonoid compounds in all the studied solvents. The plant leaf extract of S. cumini revealed the presence of four types of flavonoids compounds, Quercetin, Rutin, Ellagic acid and Kaempferol. Out of the five solvent used, it was evident that ethanolic leaf extract indicates highest quantity of Quercetin (0.19394±0.05µg/mL), Rutin was move in aqueous extract (0.04731±0.05µg/mL) whereas ethyl acetate extract exhibited highest content for Ellagic acid (0.02492±0.03µg/mL) and Kaempferol (0.02521±0.05µg/mL). The HPTLC method provides a good resolution for flavonoid compound. Separation was performed on silica gel 60 F254 HPTLC plate with Ethyl acetate: Formic acid: Acetic acid: Water in the proportion of 10:0.5:0.5:1.3 (v/v) as a mobile phase. The antimicrobial activity was carried out using five different microorganisms, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Salmonella typhi. The sonicated ethanolic leaf extract indicated high level of antimicrobial activities against Candida albicans, E. coli and S. aureus.