Author(s):
Wissam Zam, Ali Ali, Farah Husein
Email(s):
ws.sarah2005@gmail.com , w.zam@au.edu.sy
DOI:
10.5958/0974-360X.2020.00480.1
Address:
Wissam Zam1*, Ali Ali2, Farah Husein2
1Department of Analytical and Food Chemistry, Faculty of Pharmacy, Al-Andalus University for Medical Sciences, Tartous, Syria.
2Department of Food Technology, Faculty of Technical Engineering, Tartous University, Tartous, Syria.
*Corresponding Author
Published In:
Volume - 13,
Issue - 6,
Year - 2020
ABSTRACT:
Oregano and thyme are plants belonging to Lamiaceae family and are considered a rich source of polyphenols. The aim of this study is to determine the amount of polyphenols and the antioxidant capacity of oregano, thyme and their 1:1 mixture. Extracts were prepared by maceration (from 1 to 15 days) using glycerin at a concentration of 95%. The phenolic content of these extracts was calculated by Folin-Ciocalteau assay, whereas DPPH (2,2-diphenyl-1-picrylhudrazyl) test was used to determine the antioxidant capacity. Oregano had the highest phenolic content and antioxidant capacity while thyme had the lowest values. The best time of maceration was at 10 days for all extracts. In conclusion, results showed that glycerin could be used as an important green solvent in the extraction of polyphenols from plant materials; also increasing the maceration period to 10 days is better for both phenolic content and antioxidant activity.
Cite this article:
Wissam Zam, Ali Ali, Farah Husein. Extraction of Polyphenols from Oregano and Thyme by Maceration using Glycerine. Research J. Pharm. and Tech 2020; 13(6): 2699-2702. doi: 10.5958/0974-360X.2020.00480.1
Cite(Electronic):
Wissam Zam, Ali Ali, Farah Husein. Extraction of Polyphenols from Oregano and Thyme by Maceration using Glycerine. Research J. Pharm. and Tech 2020; 13(6): 2699-2702. doi: 10.5958/0974-360X.2020.00480.1 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-6-30
REFERENCES:
1. R. Amarowicz, Z.Z. Egarska, R. Rafałowski, R.B. Pegg, M. Karamac and A. Kosinska. Antioxidant activity and free radical-scavenging capacity of ethanolic extracts of thyme, oregano, and marjoram. Eur. J. Lipid Sci. Technol. 2009; 111: 1111-1117.
2. H.F. Hasan, S.L. Hassan and O.K. Luaibi. Effect of Thymus vulgaris leaves Extract and Ceftriaxone on induced Pyometra in mice. Research J. Pharm. and Tech. 2019; 12(10):4683-4688.
3. A. Vallverdú-Queralt, J. Regueiro, M. Martínez-Huélamo, J. Alvarenga, L. Leal and R. Lamuela-Raventos. A comprehensive study on the phenolic profile of widely used culinary herbs and spices: rosemary, thyme, oregano, cinnamon, cumin and bay. Food Chemistry. 2014.
4. A. Thennarasu. Quercetin in Health and Disease. Research J. Pharm. and Tech. 2013; 6(12): 1397-1399.
5. U.S. Mahadeva Rao, K.S. Mohd, S.Z. Bt Abd Halim and M. Bt Khamis. Screening of Phytochemicals and Comparative Antioxidant activity of Leaf and Fruit of Malaysian Mengkudu Using Aqueous and Organic Solvent Extracts. Research J. Pharm. and Tech. 2013; 6(9): 1064-1072.
6. A. Yashin, Y. Yashin, X. Xia and B. Nemzer. Antioxidant Activity of Spices and Their Impact on Human Health: A Review. Antioxidants. 2017.
7. Mahadeva Rao US, Mohd KS, Bt Abd Halim SZ and Bt Khamis M. Screening of Phytochemicals and Comparative Antioxidant activity of Leaf and Fruit of Malaysian Mengkudu Using Aqueous and Organic Solvent Extracts. Research J. Pharm. and Tech. 2013; 6(9): 1064-1072.
8. T. Das. Role of Antioxidants in Health and Diseases-A Review. Research J. Pharm. and Tech. 2015; 8(8): 1033-1037.
9. P. Vishnu Mohan Reddy, K. Venkata Ratnam, R.R. Venkata Raju. In vitro Antioxidant Properties of Alangium salvifolium Seed Extracts. Research J. Pharm. and Tech. 2019; 12(10):4714-4718.
10. R. Selvam, D. Anandhi, D. Saravanan, K. Revathi. Antioxidant Properties of Punica grantum Fruit rind extract against liver Hepatocellular Carcinoma studied in HEPG2 Cell Line. Research J. Pharm. and Tech. 2019; 12(10): 4719-4723.
11. T. Rababah, F. Banat, A. Rababah, K. Ereifej and W. Yang. Optimization of Extraction Conditions of Total Phenolics, Antioxidant Activities, and Anthocyanin of Oregano, Thyme, Terebinth, and Pomegranate. Journal of Food Science. 2010; 75.
12. H. Sakkas and C. Papadopoulou. Antimicrobial Activity of Basil, Oregano, and Thyme Essential Oils. J. Microbiol. Biotechnol. 2017; 27: 429-438.
13. T. Kulisic, V. Dragovi-Uzelac and M. Milos. Antioxidant Activity of Aqueous Tea Infusions Prepared from Oregano, Thyme and Wild Thyme. Antioxidant Activity of Herbal Tea Infusions, Food Technol. Biotechnol. 2006; 44: 485-492.
14. I. Mourtzinos, E. Anastasopoulou, A. Petrou, S. Grigorakis, D. Makris and C. Biliaderis. Optimization of a green extraction method for the recovery of polyphenols from olive leaf using cyclodextrins and glycerin as co-solvents. J Food Sci Technol. 2016; 53: 3939-3947.
15. B. Karakashov, S. Grigorakis, S. Loupassaki, I. Mourtzinos and D. Makris. Optimisation of organic solvent-free polyphenol extraction from Hypericum triquetrifolium Turra using Box–Behnken experimental design and kinetics. Int J Ind Chem .2015; 6: 85-92.
16. E. Shehata, S. Grigorakis, S. Loupassaki and D. Makris. Extraction optimization using water/glycerol for the efficient recovery of polyphenolic antioxidants from tow Artemisia species. Separation and Purification Technology. 2015; 149: 462-469.
17. W. Brand-Williams, M.E. Cuvelier and C. Berset. Use of a Free Radical Method to Evaluate Antioxidant Activity. Lebensm.-Wiss. u.-Technol. 1995; 28: 25-30.
18. W. Zam, G. Bashour, W. Abdelwahed and W. Khayata. Separation and Purification of Proanthocyanidins Extracted from Pomegranate’s Peels (Punica granatum). International Journal of Pharmaceutical Sciences and Nanotechnology. 2012; 5(3): 1808-1814.
19. K.V.V.S. Krishna, B. Venkatesh, A. Madhavi, B. V. Padmavathi Devi, Christina Emette Jaldi, D. Sai Bhargavi. Evaluation of Antioxidant Activity of Panchachurnam: An Ayurvedic Polyherbomineral Formulation. Research J. Pharm. and Tech. 2019; 12(10): 4841-4847.
20. M.A. Melakhessou, N. Benkiki and S.E. Marref. Determination of Antioxidant Capacity, Flavonoids and Total Phenolic Content of Extracts from Atractylis flava Desf. Research J. Pharm. and Tech 2018; 11(12): 5221-5226.
21. W. Zam, G. Bashour, W. Abdelwahed and W. Khayata. Effective extraction of polyphenols and proanthocyanidins from pomegranate’s peel. International Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4(3): 675-682.
22. A. Adámková, L. Kouřimská and B. Kadlecová. The effect of drying on antioxidant activity of selected lamiaceae herbs. Potravinarstvo® Scientific Journal for Food Industry. 2015; 9: 252-257.
23. I. Spiridon, R. Bodirlau and C. Teaca. Total phenolic content and antioxidant activity of plants used in traditional Romanian herbal medicine. Central European Journal of Biology. 2011; 6: 388-396.
24. B. Magulska and M. Wesolowski. Total Phenolic Contents and Antioxidant Potential of Herbs Used for Medical and Culinary Purposes. Plant Foods for Human Nutrition .2019; 74: 61-67.
25. I. Mourtzinos, E. Anastasopoulou, A. Petrou, S. Grigorakis, D. Makris and C.G. Biliaderis. Optimization of a green extraction method for the recovery of polyphenols from olive leaf using cyclodextrins and glycerin as co-solvents. J Food Sci Technol. 2016; 53: 3939-3947.
26. L. Mutalib. Comparison between conventional and modern methods for extraction of Rosmarinus officinalis leaves. Zanco J. Med. Sci.2015; 19.