Niladry Sekhar Ghosh, Ekta Pandey, Ritu M Giilhotra, Ranjit Singh
Niladry Sekhar Ghosh1*, Ekta Pandey2, Ritu M Giilhotra3, Ranjit Singh1
1AVIPS, Shobhit University Gangoh, Saharanpur, UP-247341, India.
2Bundelkhand Institute of Engineering and Technology, Jhansi, UP, India.
3School of Pharmacy, Suresh Gyan Vihar University, Jaipur- 302017, Rajasthan, India.
Volume - 13,
Issue - 6,
Year - 2020
Chemical, physical, and biological methods have been developed to synthesis nanoparticles but chemical and physical methods are involved in the production of toxic byproducts which are hazardous moreover the methods are very expensive. To synthesis stable metal nanoparticles with controlled size and shape, there has been search for inexpensive, safe, and reliable and “green” approach. The present study reports an environmentally friendly, low cost, novel and rapid method for synthesis of silver nanoparticles. We have developed a green synthetic method for Gold nanoparticles using Desmodium gangeticum leaf extract which act as a reducing and capping agent. It was observed that use of Desmodium gangeticum leaf extract makes rapid and convenient method for synthesis of Gold nanoparticles and can reduce Gold ions in to Gold nanoparticles within few Minutes of reaction time. The prepared AuNPs showed surface Plasmon resonance centered at 525nm with average particle size of 16±4nm. The morphological studies of the biosynthesized nanoparticles are done using UV-vis, TEM, FESEM techniques. The nanoparticles formation takes place within short time as the reaction is completed few minutes. The EDAX and XRD confirm the crystallinity of the particles. This green-clean synthetic process is conducted in natural environmental conditions. Possible mechanism of the biosynthesis is studied by FTIR. The stability studies of the colloidal nanoparticles solution are done using Zeta potential analyzers which confirm that the solution is stable for many weeks. Study of bio-functionalized AuNP is done for in-vitro free radical scavenging activity using DPPH method. In free radical scavenging study, we observed that the AuNP mopped up the free radicals in dose dependent manner.
Cite this article:
Niladry Sekhar Ghosh, Ekta Pandey, Ritu M Giilhotra, Ranjit Singh. Biosynthesis of Gold Nanoparticles using Leaf Extract of Desmodium gangeticum and their Antioxidant Activity. Research J. Pharm. and Tech 2020; 13(6): 2685-2689. doi: 10.5958/0974-360X.2020.00477.1
Niladry Sekhar Ghosh, Ekta Pandey, Ritu M Giilhotra, Ranjit Singh. Biosynthesis of Gold Nanoparticles using Leaf Extract of Desmodium gangeticum and their Antioxidant Activity. Research J. Pharm. and Tech 2020; 13(6): 2685-2689. doi: 10.5958/0974-360X.2020.00477.1 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-6-27
1. Suganthy N, Ramkumar V., Pugazhendhi, A.; Benelli, G.; Archunan, G. Biogenic synthesis of gold nanoparticles from Terminalia arjuna bark extract: Assessment of safety aspects and neuroprotective potential via antioxidant, anticholinesterase, and antiamyloidogenic effects. Environmental Science and Pollution Research. 2017.
2. Deyev, S.; Proshkina, G.; Ryabova, A.; Tavanti, F.; Menziani, M.C.; Eidelshtein, G.; Avishai, G.; Kotlyar, A. Synthesis, Characterization, and Selective Delivery of DARPin-Gold Nanoparticle Conjugates to Cancer Cells. Bioconjugate Chemistry 2017.28; 2569–2574.
3. Khutale, G.V.; Casey, A. Synthesis and characterization of a multifunctional gold-doxorubicin nanoparticle system for pH triggered intracellular anticancer drug release. European Journal of Pharmaceutics and Biopharmaceutics. 2017. 119;37-46.
4. Kumar, V.K.; Gopidas, K.R. Synthesis and characterization of gold-nanoparticle-cored dendrimers stabilized by metal-carbon bonds. Chem. Asian J. 2010. 5; 887–896.
5. Laura Castro, M. Luisa Blázquez, Felisa Gonzalez, Jesus A. Munoz, Antonio, Ballester Extracellular biosynthesis of gold nanoparticles using sugar beet pulp, Chemical Engineering Journal 2010; 164: 92-97.
6. Lee, J.; Chatterjee, D.K.; Lee, M.H.; Krishnan, S. Gold nanoparticles in breast cancer treatment: Promise and potential pitfalls. Cancer Letters. 2014. 347; 46–53.
7. Qian, X.; Peng, X.H.; Ansari, D.O.; Yin-Goen, Q.; Chen, G.Z.; Shin, D.M.; Yang, L.; Young, A.N.; Wang, M.D.; Nie, S. In vivo tumor targeting and spectroscopic detection with surface-enhanced Raman nanoparticle tags. Nat. Biotechnol. 2008, 26, 83–90.
8. Gurunathan S., Park J.H., Han J.W., Kim J.H. Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in MDA-MB-231 human breast cancer cells: Targeting p53 for anticancer therapy. Int. J. Nanomed. 2015; 10:4203–4222.
9. Singhal, G.; Bhavesh, R.; Kasariya, K..; Sharma, A.R.; and Singh, R.P. (2011). Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. Journal of Nanoparticle Research, 13(7), 2981-2988.
10. Srivastava P., Screening and Identification of Salicin Compound from Desmodium gangeticum and its in vivo Anticancer Activity and Docking Studies with Cyclooxygenase (COX) Proteins from Mus musculus. J Proteomics Bioinformatics.2013; 109-124
11. Srivastava P., Singh B.D. and Tiwari K.N., Comparative in vitro regeneration study of mature and juvenile nodal explants and extraction, isolation, characterization of bio-active constituents from leaves of an endangered medicinal plant Desmodium gangeticum (L.) DC, Res. J. Chem. Environ. 2014. 18(2);1-15
12. Srivastava P., Srivastava G., Singh B.D. and Singh S.K., Comparative evaluation of anticancer activity of crude extracts and isolated compound salicin of Desmodium gangeticum (L) DC against Ehrlich ascites carcinoma in Swiss Albino mice. World J Pharmaceutical Research.2014. 4; 2883-2898
13. Srivastava P., Singh B.D., Tiwari K.N. and Srivastava G., High frequency shoot regeneration for mass multiplication of Desmodium gangeticum (L.) DC—An important anticancer, antidiabetic and hepatoprotective endangered medicinal plant. International Journal of Scientific Research.2015. 4, 508-512.
14. Govindarajan R., Vijayakumar M., Rao C.V., Shirwaikar A., Kumar S., Rawat A.K. and Pushpangadan P., Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats. Phytotherapy Research.2003. 21(10); 975-979
15. Arabshahi D.S., Devi D.V. and Urooj A., Evaluation of antioxidant activity of some plant extracts and their heat, pH and storage stability. Food Chemistry.100;1100-1105.
16. Inbakandan D, Venkatesan R, Ajmal Khan S, Biosynthesis of gold nanoparticles utilizing marine sponge Acanthella elongate (Dendy, 1905), Colloids and Surfaces B: Biointerfaces, 2010; 81:634–639.
17. Rocktotpal Konwarh, Biswajit Gogoi, Ruby Philip, Laskar M.A, Niranjan Karaka, Biomimetic preparation of polymer-supported free radical scavenging, cytocompatible and antimicrobial “green” silver nanoparticles using aqueous extract of Citrus sinensis peel, Colloids and Surfaces B: Biointerfaces, 2011;84:338-345
18. Cotelle, A., Bernier, J.L., Catteau, J.P., Pommery, J., Wallet, J.C. and Gaydou, E.M. Antioxidant properties of hydroxyl flavones” Free. Radic. Biol. Med. 1996,20, 35-43.
19. Song, J.Y.; and Kim, B.S. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess and Biosystems Engineering. 2015. 32(1); 79-84.
20. Begum S, Hassan SI, Ali SN, Siddiqui BS. Nat Prod Res. 2004; 18(2):135.
21. ASTM Standard D 4187-82, American society for testing and Materials, 1985.