P. Maheswari, K. Arjun Kumar, S. Sankaralingam, N. Sivakumar
P. Maheswari1, K. Arjun Kumar1, S. Sankaralingam2, N. Sivakumar3
1Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi, Tamil Nadu, India.
2Department of Botany, Saraswathi Narayanan College, Madurai, Tamil Nadu, India.
3School of Biotechnology, Department of Molecular Microbiology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
Volume - 13,
Issue - 6,
Year - 2020
Exopolysaccharides are sugar containing compounds that are produced inside the bacteria and secreted into the outer environment. Mainly they are the capsular materials of the bacteria being acting as protective layer in bacteria. Polysaccharides are said to have various applications in the field of medicine, food, agriculture and so on. The polysaccharide producing bacteria was obtained from the marine sediment region and was optimized for its production in the media. The mass production yielded about 2grams of polysaccharide. The polysaccharide was characterized using FT - IR, XRD and GC – MS.
Cite this article:
P. Maheswari, K. Arjun Kumar, S. Sankaralingam, N. Sivakumar. Optimization and Characterization of Exopolysaccharide from Marine Soil Bacteria. Research J. Pharm. and Tech 2020; 13(6):2540-2544. doi: 10.5958/0974-360X.2020.00452.7
1. Ahmed Nasr Mohamed, M.Ranjard, L., Catroux, C., Catroux, G., Hartmann, A. 2005. Effect of natamycin on the enumeration, genetic structure and composition of bacterial community isolated from soils and soybean rhizosphere. J Microbiol Meth.60: 31-40.
2. Burgula, Y., D, Khali., S, Kim., S. S, Krishnan., M. A, Cousin., J. P. Gore., B. L, Reuhs and L. J. Mauer.2007. Review of Mid-Infrared Fourier Transform-Infrared Spectroscopy Applications for Bacterial detection. Journal of Rapid methods and Automation in Microbiology.15: 146-175.
3. Datta, K.K.R., Reddy, B.V.S., Zboril, R.2016. Polysaccharides as functional scaffolds for noble metal nanoparticles and their catalytic applications. In Encyclopedia of Nanoscience and Nanotechnology, American Scientific Publishers: Valencia, CA, USA, 1–20
4. Dogsa, I., Kriechbaum, M., Stopar, D., Laggner.2005. P. Structure of bacterial extracellular polymeric substances at different pH values as determined by SAXS. Biophys. J.89, 2711–2720.
5. Liu, S.B., Qiao, L.P., He, H.L., Zhang, Q. and Chen, X.L. 2011. Optimization of fermentation conditions and rheological properties of exopolysaccharide produced by Deep-Sea bacterium Zunongwangiaprofunda SM-A87. PLoS ONE.6(11): 268-273.
6. Madhurik, V and K, Vidyaprabhakar. 2014.Microbial Exopolysaccharides: Biosynthesis and Potential Applications, Orient. J. Chem.Vol. 30(3), 1401-1410.
7. Maeda, H., Wu, J., Sawa, T., Matsumura, Y., and Hori, K. 2000.Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review. J. 65:271-84
8. Maheswari, P. and M. Karthiga.2016. Media optimization of exopolysaccharide producing bacterium from marine sediment. International Journal of Scientific and Engineering Research., Volume 7, Issue 6.
9. Nadia Ruocco., Susan Costantini., Stefano Guariniello and Maria Costantini. 2016. Polysaccharides from the Marine Environment with Pharmacological, Cosmeceutical and Nutraceutical Potential. Molecules MDPI. 21 (551): 1-29.
10. Shankar, T., Sivakumar, T., Vijayabaskar, P., Babinastarlin, S and Anandapandian, K.T.K. 2011.Quantification and Characterization of Exopolysaccharides from Bacillus subtilis (MTCC 121). Advances in Biological Research.5 (2): 71-76.
11. Uchenchukwu, U., Nwodo., Ezeikel Green and Anthony. I., Okoh. 2015. Bacterial Exopolysaccharides: Functionality and Prospects. International Journal of Molecular Sciences.13: 14002-14015.
12. Zhemning, C., Yan, F. 2005. Exopolysaccharides from marine bacteria. J. Ocean Univ. China. 4, 67–74.