Author(s): Rohit Kumar Bargah, Anil Kushwaha, Arun Tirkey, Bimlesh Hariwanshi


DOI: 10.5958/0974-360X.2020.00466.7   

Address: Rohit Kumar Bargah*, Anil Kushwaha, Arun Tirkey, Bimlesh Hariwanshi
Department of Chemistry, Govt. S.P.M. College, Sitapur, Surguja (C.G.) India , 497111.
*Corresponding Author

Published In:   Volume - 13,      Issue - 6,     Year - 2020

Nature has been a powerful source of enormous medicines for thousands of years and number of modern drugs has been extracted and exploited from natural sources, which are used in traditional systems of medicines. The main aim of the present study was to evaluate. In- Vitro antioxidant, antibacterial activity and preliminary phytochemical screening of the ethanolic and aqueous extract of flowers of Cassia auriculata Linn. Belonging to the family Caesalpiniaceae. Phytochemicals screening of the crude extract revealed the presence of flavonoids, steroids, saponins, phenolic compound, terpenoids, carbohydrates, were present in all extract while alkaloids and tannins were absent only in ethanolic extract. The antioxidant activity of flowers of Cassia auriculata was assessed with the help of antioxidant activities like 2, 2-Diphenyl-1-picryl hydrazyl (DPPH) scavenging assay. Total phenol and flavonoids content were measured. Flowers show most antioxidant activity in both extracts due to higher phenolic and flavonoids content that indicated the direct correlation among antioxidant potential, total phenol and flavonoids. The presence of the bioactive constituents have been linked to the antimicrobial activity of the plant materials against gram positive and gram negative bacteria using agar disc diffusion method. Minimum inhibition concentration value ranged from 50, 75, 100 µg/ml.Ethanolicextract exhibited significantly good antibacterial activity in dose depended manner. Therefore, it is concluded that Cassia auriculata possess phenolic properties that has considerable antioxidant and antibacterial properties.

Cite this article:
Rohit Kumar Bargah, Anil Kushwaha, Arun Tirkey, Bimlesh Hariwanshi. In Vitro Antioxidant and Antibacterial Screening of flowers Extract from Cassia auriculata Linn. Research J. Pharm. and Tech 2020; 13(6):2624-2628. doi: 10.5958/0974-360X.2020.00466.7

01    Anushia C., Sampath kumar P., Ramkumar L.; Antibacterial and Antioxidant activities in Cassia auriculata; Glob J.Pharmacol.2009; l3:127-130.
02    Dr. Satya prakash, Wealth of India: Raw materials Publication and Information Directorate, New Delhi, Council of Scientific and Industrial Research 1950; 247.
03    Madkour FF, Abdel – Daim MM; Hepatoprotective and Antioxidant activity of Dunaliella salina in Paracetamol Induced acute toxicity in rats, Indian J. Pharm. Sci, 2013; 75:642-648.
04    Soundharajan SR, Ponnusamy RD; Chemical Composition and Characterization Studies of Cassia auriculata flower extract.Int. J. Pharm.Sci.2014; 6:327-330.
05    Rajgopal P., Reshmi V., Sreejith K., Kiran S., AneeshaS., Premlatha K.; “Cassia auriculata a Phytopharmecological Review”, American J. Pharm. Health Res., 2014; 2:1-20.
06    Anandan A., Eswaran R., Doss A., Sangeetha G., Anand S.; Chemical Compound Investigation of Cassia auriculata Leaves a potential folklore Medicinal Plants; Bull. Environ Pharmacol. Life Sci., 2011; 1:20-30.
07    Basu and Kirtikar; Indian Medicinal Plant, Vol.II, Second edition, International Book Distributiors Dehradun India.1935; 867-868.
08    Subedi L., Timalsena S., Dewadi P., Thapa R., Paudel A. and Prajuli K.; “Antioxidant activity and Phenol and Flavonoid contain of eight medicinal plant from western Nepal, J. Tradit. Clin. Med., 2014; 34:584-590.
09    Gaurav MO, Mukesh DS; Journal of Pharmaceutical Research and Clinical Practice; 2011; 2:50-58.
10    Sushma.K., Praveen K.; Pakistan Journal of Biological Science; 2012; 15(9):408-412.
11    Kulkarani A., Govindappa M., Channa Basava C., Ramchandra Y., Koka P.; “Phytochemical analysis of Cassia species and its In-vitro antimicrobial, antioxidant and anti-inflammatory activities”, Adv. Med. Plant Res; 2015; 3:8-17.
12    Kumaran, A., and Joel Karuna karan R.; Antioxidant activity of Cassia auriculata flower; Fitoterapia, 2007; 78[1]: 46-47.
13    Sabu, MC and T.Subburaju; “Effect of Cassia auriculata Linn-on serum glucose level, glucose utilization by isolated rat Hemi diaphragm, Journal of Ethanopharmacol.2002; 80: 203-206.
14    Harbone JB; Phytochemical Method: Chapmann and Hall, Landon, 1998(1-27).
15    Mc. Donald S., Prenzier PD., Antolovich M., Robards K. Phenolic Content and antioxidant activity of olive extracts; Food Chem., 2001; 73:73-84.
16    Chang C. Yang M., Wen, H.; Estimation of Total Flavonoids content –in propolis by two complement any colorimetric method.J. Food DrugAnal., 2002; 10: 178-182.
17    Bauer, AW, Kirby WMM, Sherries JC, Turck M.; “Antibiotics susceptibility testing by a stain oxidized single disc method”. Am.J. Clin. Pathol.; 1966; (4): 493-496.
18    Benignant, R., Sang G., Crisafi M.P; Germano R. Evaluation of medicinal plant products as antimicrobial agent. phytoth. Res, 1996; 12:1, 154-56.
19    Btranter, A. and E-Grein; “Antibacterial activity of plant extract used externally in traditional medicine, J. Ethanopharmacol., 1994; 44 35-40.
20    Cottelle, N., Coitelle A., Berrier, JL., Catteau, JP, Pommery, J., Wallet, JC and Gacydou EM; Antioxidant properties of hydroxyl –flavones Free radical Bio.Med.1996; 1 (20) 35-43.
21    Burits M., Bucar F.; “Anioxidant activity of Plant: Uigella savita essential oil” Phytother. Res. 2002; (14):323-338.
22    Anushia CP, Sampth Kumar and L. Ramkumar; “Antibacterial and Antioxidant activity in Cassia auriculata; Global Journal of Pharmacol.2009; 3:127-130.
23    Desai PV, Wadekar RR, Free Radical Scavenging activity of aqueous extract of roots of Baliospermum montarum muell-Arg, International Journal of Green Pharmacy. 2008; 2(1):31-33.
24    Cowan, MM; Plant Products as antimicrobial agents; Clnin Microbial.Rev.1999; 12:264-82.
25    Shahidi, F. and, Wanasunda P.K.; Phenolic antioxidants; Food Sci Nuter.1992; 32 : 67-103.
26    Dioxin RN, XieD.Y., Sharma S.B., A Final Frontier in Flavonoids Research, New Phytol, 2005; 165:9-28.
27.  Bargah, RK and Kushawaha PK; Extraction, Phytochemical Screening and In-Vitro Antioxidant activity of Cassia fistula extracts; International Journal of Research in Pharmacy and Chemistry; 2017; 7(4): 518-524.

Recomonded Articles:

Author(s): Rakhi Mehra, Renu Makhija, Neera Vyas

DOI: Not Available         Access: Open Access Read More

Author(s): Niha Naveed, Karthikeyan Murthykumar, Subasree Soundarajan, Sripradha Srinivasan

DOI: Not Available         Access: Open Access Read More

Author(s): U.S Mahadeva Rao, Khamsah Suryati Mohd, Abdurrazaq Muhammad, Bashir Ado Ahmad, Mohaslinda Mohamad, Rosmawati Mat Ali

DOI: Not Available         Access: Open Access Read More

Author(s): Sandeep D. S, Prashant Nayak, Jobin Jose, Rishal Relita M, Sumana D. R.

DOI: 10.5958/0974-360X.2017.00317.1         Access: Open Access Read More

Author(s): Ankit Patel, Pankaj Kushwah, Sujit Pillai, Ajay Raghuvanshi, Nitin Deshmukh

DOI: 10.5958/0974-360X.2017.00013.0         Access: Open Access Read More

Author(s): Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah

DOI: Not Available         Access: Open Access Read More

Author(s): Praveen D, Ranadheer Chowdary P, Gandikota Thanmayi, Gangabathina Poojitha, Vijey Aanandhi M

DOI: 0.5958/0974-360X.2016.00047.0         Access: Open Access Read More

Author(s): Vandana Gautam, Dhriti Kapoor, Saroj Arora, Renu Bhardwaj*

DOI: 10.5958/0974-360X.2016.00166.9         Access: Open Access Read More

Author(s): Dhanalekshmi UnniKrishnan Nair, MP Saraswathy, Narra Kishore, Neelakanta Reddy Pully

DOI: Not Available         Access: Open Access Read More

Author(s): S. R Suseem, Dhanish Joseph

DOI: 10.5958/0974-360X.2019.00067.2         Access: Open Access Read More

Author(s): A. Julius, Ramachandran Vedasendiyar, Archana Devakannan, Sujatha Rajaraman, Balamurugan Rangasamy, V. Saravanan

DOI: 10.5958/0974-360X.2017.00062.2         Access: Open Access Read More

Author(s): Malarkodi Velraj, P. Jasmine Shiney, Biplab Paul, Rashmi. S, Nivethitha

DOI: 10.5958/0974-360X.2017.00006.3         Access: Open Access Read More

Author(s): B. Thawkar, M. Kale, M. Oswal, K. Maniyar, K. Kadam, S. Kamat

DOI: 10.5958/0974-360X.2016.00044.5         Access: Open Access Read More

Author(s): Hayat M. Mukhtar, Vandna Kalsi

DOI: 10.5958/0974-360X.2018.00395.5         Access: Open Access Read More

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles