Author(s): Chandiran Sharmila, Krishnamoorthy Renuka, Sorimuthu Pillai Subramanian


DOI: 10.5958/0974-360X.2020.00465.5   

Address: Chandiran Sharmila, Krishnamoorthy Renuka, Sorimuthu Pillai Subramanian*
Department of Biochemistry, University of Madras, Guindy Campus, Chennai- 600025.
*Corresponding Author

Published In:   Volume - 13,      Issue - 6,     Year - 2020

Type 2 diabetes mellitus accounts for more than 90% of the total diabetic population worldwide. Though several drugs having a different mechanism of actions are commercially available to maintain normoglycemia in diabetic individuals, none is found to be ideal due to undesirable side effects in addition to the development of drug resistance after prolonged use. This scenario necessitates the search for new drugs with maximum efficacy at a relatively low dose and without side effects. Medicinal plants serve as an immense source of new lead molecules and more than 60% of the currently available drugs are originally identified from the medicinal plants. The present study aims to systematically evaluate the anti diabetic properties of Strychnos potatorum Linn. seeds which have been traditionally used for the treatment for diabetes and its secondary complications. The Phytochemical screening and the total phenolic, flavonoids, carbohydrate and protein contents evidenced the presence of biologically important phytochemicals in the seeds extract. Acute toxicity and dosage fixation studies revealed the non-toxic nature of the seeds extract and oral administration of the ethanolic extract of seeds at a concentration of 500mg/kg/bw for 30 days to High Fat Diet Fed- low dose streptozotocin induced experimental type 2 diabetic rats significantly ameliorates the altered biochemical indices such as fasting blood glucose, glycosylated hemoglobulin, plasma insulin by improving the insulin levels as well as by regulating the activities of key enzymes of carbohydrate and glycogen metabolism. The assay of pathophysiological enzymes such as AST, ALT and ALP revealed the non-toxic nature of the seeds extract. The data obtained provide evidence for the folklore use of the seeds in the traditional medicine for the treatment of diabetes mellitus.

Cite this article:
Chandiran Sharmila, Krishnamoorthy Renuka, Sorimuthu Pillai Subramanian. Biochemical Evaluation of Antidiabetic properties of Strychnos potatorum Seeds extract studied in High Fat Diet Fed- Low dose Streptozotocin induced experimental type 2 diabetes in Rats. Research J. Pharm. and Tech 2020; 13(6):2615-2623. doi: 10.5958/0974-360X.2020.00465.5

Chandiran Sharmila, Krishnamoorthy Renuka, Sorimuthu Pillai Subramanian. Biochemical Evaluation of Antidiabetic properties of Strychnos potatorum Seeds extract studied in High Fat Diet Fed- Low dose Streptozotocin induced experimental type 2 diabetes in Rats. Research J. Pharm. and Tech 2020; 13(6):2615-2623. doi: 10.5958/0974-360X.2020.00465.5   Available on:

1.    Chawla A, Chawla R, Jaggi S. Microvasular and macrovascular complications in diabetes   mellitus: Distinct or continuum?. Indian J Endocrinol Metab. 2016;20(4):546–551.
2.    Raptis SA, Dimitriadis GD. Oral hypoglycemic agents: insulin secretagogues, alpha-glucosidase inhibitors and insulin sensitizers. Exp Clin Endocrinol Diabetes. 2001;109(2):265-87.
3.    Dhanish J, Junia G, Manju M M, Flowerlet M, Bimi V, Betsy S. A Compilation on Anti-Diabetic Profile of Cocos nucifera. Research Journal of Pharmacy and Technology.2019; 12(8): 3791-3796.
4.    Sharmila C, Subramanian SP, Studies on the Defluoridization Competency of a Mixture of Raw Vermiculite and Strychnos potatorum linn. Seeds. Der Pharma Chemica. 2019;11(4): 38-43.
5.    Madhuri M, Deshmukh, Chhaya S. Ambad, Nutan Kendre, Navnath G. Kashid. Biochemical Screening, Antibacterial and GC-MS Analysis of Ethanolic Extract of Hemidesmus indicus (L) R.Br. root. Res.  J. Pharmacognosy and Phytochem. 2019; 11(2):73-80.
6.    Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, et al. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol. 2000; 72 (1-2): 35-42.
7.    Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-275.
8.    OECD. Guideline Number 423 for the Testing of Chemicals: Revised Draft Guideline 423 (Acute Oral Toxicity).Paris. France: OECD; 2000.
9.    Suman RK, Mohanty IR, Borde MK, Maheshwari U, Deshmukh YA. Development of an experimental model of diabetes co-existing with metabolic syndrome in rats. Advances in Pharmacological Sciences. 2016; 1-11.
10.    Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: A model for Type 2 diabetes and pharmacological screening. Pharmacol Res. 2005;52:313-20.
11.    Mather K. Surrogate measures of insulin resistance: of rats, mice, and men. Am J Physiol Endocrinol Metab. 2009; 296(2):398-9.
12.    Matthews DR, Hosker JP, Rudenski AS, et al. Homeostasis model assessment: Insulin resistance and beta-cell function fromfasting plasma glucose and insulin concentrations in man. Diabetologia. 1985; 28:412–19.
13.    Yusuf-Babatunde AM, Osuntokun, OT, Ige, OO, Solaja OO. Secondary metabolite Constituents, Antimicrobial Activity and Gas chromatography-Mass spectroscopy Profile of Bombax buonopozense P. Beauv. (Bombacaceae) Stem bark Extract. Res.  J. Pharmacognosy and Phytochem. 2019; 11(2):87-92.
14.    Manish Pal Singh and Kamla Pathak. Animal models for biological screening of anti-diabetic drugs: An overview. European Journal of Experimental Biology, 2015, 5(5):37-48.
15.    Andallu B, Varadacharyulu NCh. Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats. Clin Chim Acta. 2003;338 (1-2):3-10.
16.    American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. Diabetes Care. 2013;36(1): S67–S74.
17.    Braunlich H, Marx F, Fleck C, Stein G. Kidney function in rats after 5/6 nephrectomy (5/6 NX); effort of treatment with vitamin E. Exp Toxicol Pathol. 1997;49:135 - 139.
18.    Kavanagh KL, Elling RA, Wilson DK. Structure of Toxoplasma gondii LDH1: active-site differences from human lactate dehydrogenases and the structural basis for efficient APAD+ use. Biochemistry. 2004;43(4):879-89.
19.    Minassian C, Mitheux G. Differential time course of liver and kidney glucose-6-phospahtase activity during fasting in rats. Comp Biochem physiol B: Biochem Mole Biol. 1994; 109: 99–104.
20.    Aoki K, Saito T, Satoh S, Mukasa K, Kaneshiro M, Kawasaki S, Okamura A, Sekihara H. Dehydroepiandrosterone suppresses the elevated hepatic glucose-6-phosphatase and fructose-1,6-bisphosphatase activities in C57BL/Ksj-db/db mice: comparison with troglitazone. Diabetes. 1999;48: 1579-85.
21.    Whitton, Hems D A. Glycogen synthesis in the perfused liver of streptozotocin-diabetic rats. Biochem J. 1975; 150(2): 153–165.
22.  Jung UJ, Lee MK, Jeong KS, Choi MS. The hypoglycemic effects of hesperidin and naringin are partly mediated by hepatic glucose-regulating enzymes in C57BL/KsJ-db/db mice. J Nutr. 2004; 134(10):2499-503.

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