Author(s):
Galymzhan Burayev, Medet Khamitov, Vassily Lozovoy, Rasulbek Aipov, Elena Lozovaya, Lyudmila Akhmaltdinova, Kamshat Burayeva
Email(s):
buraev.galymzhan@mail.ru
DOI:
10.5958/0974-360X.2020.00463.1 
Address:
Galymzhan Burayev1*, Medet Khamitov1, Vassily Lozovoy1, Rasulbek Aipov1, Elena Lozovaya1, Lyudmila Akhmaltdinova2, Kamshat Burayeva3
1Department of Pediatric Surgery, Astana Medical University - 010000 Nur-Sultan, Kazakhstan.
2Department of Immunology, Karaganda State Medical University - 100000 Karaganda, Kazakhstan.
3Department of Therapy, Khoja Akhmet Yassawi International Kazakh-Turkish University - 161200 Turkestan, Kazakhstan.
*Corresponding Author
Published In:
Volume - 13,
Issue - 6,
Year - 2020
ABSTRACT:
The purpose of the article is to study the state of the intestinal microflora in the context of chronic colonic stasis and assess the methods of its correction by means of siphon enema and probiotics’ implication (bifidumbacterin and lactobacterin) in order to prevent the development of HAEC. This study was conducted in the Astana City Children's Hospital No. 2 and involved 60 children, who applied for medical help with symptoms of chronic constipation. They were admitted to the hospital in a planned or emergency manner with suspected Hirschsprung's disease in the period from 2015 to 2017, after approval of the Ethics Committee. An X-ray contrast study of the large intestine (irrigography) was performed in all the children for a diagnostic purpose. The following parameters of the state of the large intestine were studied: the function of the ileocecal valve (Bauhin'svalve), the diameter and shape of the large intestine, the presence of pathological formations, symptoms of intestinal obstruction. A statistical analysis was carried out using Student's test for dependent and independent samples by means of the Biostatsoftware. After treatment, the bacteriological examination of stool samples demonstrated an increase in the number of beneficial microorganisms and a decrease in the number of opportunistic microorganisms. The growth in the number of patients with the normal intestinal microflora (14 patients – 70%) was observed along with the reduction in the number of patients with intestinal dysbiosis, with a statistically significant difference (p<0.001). The developed method of correcting the quantitative composition of the intestinal microflora is very effective and can be implemented in clinical practice.
Cite this article:
Galymzhan Burayev, Medet Khamitov, Vassily Lozovoy, Rasulbek Aipov, Elena Lozovaya, Lyudmila Akhmaltdinova, Kamshat Burayeva. The Correction Methods of the Intestinal Microflora in Chronic Colonic Stasis by Siphon Enema and Probiotics as a Means of the Hirschsprung-associated Enterocolitis Prevention. Research J. Pharm. and Tech 2020; 13(6):2601-2607. doi: 10.5958/0974-360X.2020.00463.1
Cite(Electronic):
Galymzhan Burayev, Medet Khamitov, Vassily Lozovoy, Rasulbek Aipov, Elena Lozovaya, Lyudmila Akhmaltdinova, Kamshat Burayeva. The Correction Methods of the Intestinal Microflora in Chronic Colonic Stasis by Siphon Enema and Probiotics as a Means of the Hirschsprung-associated Enterocolitis Prevention. Research J. Pharm. and Tech 2020; 13(6):2601-2607. doi: 10.5958/0974-360X.2020.00463.1 Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-6-13
REFERENCES:
1. Kenny SE, Tam PK, Garcia-Barcelo M. Hirschprung’sdisease, Semin. Pediatr. Surg. 2010; 19: 194-200.
2. McKeown SJ, Stamp L, Hao MM, Young HM. Hirschsprung disease: A developmental disorder of the enteric nervous system. Wiley Interdiscip Rev Dev Biol. 2013; 2: 113-29.
3. Langer JC. Hirschsprung disease. CurrOpinPediatr. 2013; 25: 368-74.
4. Kholostova VV. 2015. Surgical treatment of Hirschsprung's disease in children: Doctor of Medical Sciencesdis.: 14.01.19. Moscow. N.I. Pirogov Russian National Research University.
5. Krivcheniya DYu, Khursin VN, Pritula VP. Enterocolitis in Hirschsprung disease. Immunohistological features, pathogenesis, clinic, treatment. Ukrainian Medical Journal. 2001; 5(25): 10.
6. Baranovskiy AYu. Fundamentals of nutrition of Russians: Therapeutic starvation. Nutrition and pregnancy. Eating after surgery. Therapeutic nourishment. Diets. Food intolerance: Directory. St. Petersburg [and others]: Peter. 2007.
7. Bondarenko VM. Dysbacteriosis of the intestine as a clinical and laboratory syndrome: the current state of the problem. Manual for doctors. Moscow: GEOTAR-Media. 2007.
8. Gosain A, Brinkman AS. Hirschsprung's associated enterocolitis. Curr. Opin. Pediatr. 2015; 27(3): 364-369.
9. Austin KM. The pathogenesis of Hirschsprung`s disease-associated enterocolitis. Semin Pediatr. Surg. 2012; 21: 319-27.
10. Lenyushkin AI. Surgical coloproctology of childhood. Moscow: Medicine. 1999.
11. Kivva AN. Monitoring of the level of endogenous intoxication in Hirschsprung disease in children. Children's surgery. 2001; 6: 15-17.
12. Kolomeitsev PA. Chronic constipation. Bulletin of Surgery. 1999; 158(6): 52.
13. Reid JR. The barium enema in constipation comparison with rectal manometry and biopsy to exclude Hirschsprung's disease after the neonatal period. Padianradiol. 2000; 30(10): 681-684.
14. Sectoral standard “Protocol of treatment. Intestinal dysbiosis”, №231 from 9.06.2003. Order of the Ministryof Health of Russian Federation. Available from: https://www.evrika.ru/federal-standards/620 [Last accessed on 15 Apr 2019]
15. Kulchenko NG, Chibisov SM, Eremina I, Vekilyan MA, Syatkin SP, Skorik AS, Myandina GI, Shevkun NA, Neborak EV. Dynamic Changes of the Microflora Sensitivity to Antibiotics in Patients with Chronic Calculous yelonephritis. Research J. Pharm. and Tech. 2017; 10(3): 683-686.
16. Sanal Kumar MG, Nandakumar S, Bini B, Mahitha MS. Concentration Study of Antimicrobial Activity with Selected Oral Cleansers on Oral Microflora. Research J. Pharm. and Tech. 2016; 9(12): 2241-2245.
17. Sai ChRB, Gheena S. Comparison of Microflora in Patients with Controlled and Uncontrolled Diabetes. Research J. Pharm. and Tech. 2015; 8(8): 1184-1186.
18. Hossain MdK, Nahar K, Shokryazdan P, Abdullah N, Hamid K, Jahromi MF. Probiotic Potential of Lactic Acid Bacteria Isolated from Cheese, Yogurt and Poultry Faeces. Research J. Pharm. and Tech. 2017; 10(9): 2991-2998.
19. Bhagat BV, Jagtap AR, Hapse SA, Pagar HJ, Wagh AS., Kale NR. Formulation and Evaluation of Probiotic Gel Containing Anti-inflammatory agent for Treatment of IBDS. Research J. Pharm. and Tech. 2012; 5(3): 387-389.
20. Shinde N, Bangar B, Deshmukh S, Kumbhar P. Nutraceuticals: A Review on current statusResearch J. Pharm. and Tech. 2014; 7(1): 110-113.
21. Indira M, Venkateswarulu TC, Chakravarthy K, Ranganadha Reddy A, Vidya Prabhakar K. Isolation and Characterization of Bacteriocin Producing Lactic Acid Bacteria from Diary Effluent. Research J. Pharm. and Tech. 2015; 8(11): 1560-1565.
22. Swathi KV. Probiotics –A Human Friendly Bacteria. Research J. Pharm. and Tech. 2016; 9(8): 1260-1262.
23. Moorthy MK, Nayak BK, Nanda A. Antioxidant and antitumor activity of lactic bacteria isolated from natural beverage - Coconut toddy. Research J. Pharm. and Tech. 2017; 10(12): 4317-4320.
24. Cheryomushkina IV, Korneeva OS. Innovative Biotechnology Probiotic Feed Additives C and Immunostimulatory Effects. Research J. Pharm. and Tech. 2017; 10(4): 1165-1167.
25. Ursova NI. Disturbance of microflora and dysfunction of the biliary tract in children: a guide for practicing doctors. Ed. GVRimarchuk. Moscow: Prototype. 2005.
26. Fuller R. Probiotics in man and animals. Journal of Applied Bacteriology. 1989; 66(5): 365-378.
27. Elisseyev YY. Violation of intestinal microflora (dysbacteriosis), childhood diseases. Complete Reference Book. Moscow: Litres. 2008.
28. Dysbacteriosis. Mythical diagnosis. Health by Nature. Available from: http://www.russianweek.ca/articles/experts-advice/staroverskaya/disbakterioys/ [Last accessed on 15 Apr 2019]
29. Shen DH, Shi GR, Chen JJ. Detection of intestinal bifidobacteria and lactobacilli in patients with Hirschsprung's disease associated enterocolitis. World J Pediatr WJP. 2009; 5: 201-205.
30. El-Sawaf M, Siddigui S, Mahmoud M. Probiotic prophylaxis after pullthrough for Hirschsprung disease to reduce incidence of enterocolitis: a prospective, randomized, double-blind, placebo-controlled, multicenter trial. J Pediatr Surg. 2013; 48: 111-117.