Author(s): G. B. Jadhav, A. C. Deshmukh, K. N. Mundlod

Email(s): aaryajadhav@rediffmail.com

DOI: 10.5958/0974-360X.2020.00378.9   

Address: G. B. Jadhav*, A. C. Deshmukh, K. N. Mundlod
Department of Pharmacology, MVP’s College of Pharmacy, Gangapur Road , Nashik, Affiliated to S.P. Pune University, (M.S.), India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 5,     Year - 2020


ABSTRACT:
Diabetic neuropathy is common microvascular complication of diabetes mellitus. Objective of research was to evaluate the effect of Linagliptin and Niclosamide on Streptozotocin (STZ) induced neuropathy in experimental diabetic Wistar Rats. Here, animals were fed with high fat diet for about two weeks after that injected with streptozotocin 30 mg/kg i.p. dual dose in consequent week for production of diabetes. Animals were separated into eight groups. Locomotor activity, Tail immersion, hot plate and motor coordination after two week of diabetes induction were used for assessed Diabetic neuropathy. Disease animals treated with Linagliptin (3, 5mg/kg p.o) and Niclosamide (30, 75mg/kg i.p.) and their combination as Linagliptin + Niclosamide (5, 75mg/kg). Pregabalin(10 mg/kg i.p.) given as standard to one group. At the end of treatment to measure serum glucose, cholesterol, triglyceride level blood sample was taken. Sciatic nerve was removed and investigates for level of antioxidant such as SOD, CAT, LPO, NO etc. In order to assess role of Linagliptin and Niclosamide in the relevant activity, further histopathological studies provide a supportive evidence for this study to show protective effect of drugs. Treatment with Linagliptin and Niclosamide gives protective effect in neuropathy associated with streptozotocin induced diabetes in rats. Their combination for four week showed significant fall in serum glucose, cholesterol, triglyceride level and also improve SOD, CAT level while significant fall in LPO and NO level in sciatic nerve. Further combination showed marked improvement. Thus, combination of these drugs could be better in ameliorating diabetic neuropathy in STZ induced diabetes.


Cite this article:
G. B. Jadhav, A. C. Deshmukh, K. N. Mundlod. Effect of Linagliptin and Niclosamide on Streptozotocin Induced Diabetic Neuropathy in Rats. Research J. Pharm. and Tech 2020; 13(5): 2101-2106. doi: 10.5958/0974-360X.2020.00378.9

Cite(Electronic):
G. B. Jadhav, A. C. Deshmukh, K. N. Mundlod. Effect of Linagliptin and Niclosamide on Streptozotocin Induced Diabetic Neuropathy in Rats. Research J. Pharm. and Tech 2020; 13(5): 2101-2106. doi: 10.5958/0974-360X.2020.00378.9   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-5-8


REFERENCES:
1.    Pasnoor M, Dimachkie MM, Kluding RJ. Diabetic Neuropathy Part 1: Overview and Systemic Phenotype. Neurolclin. 2013; 31: 425-455.
2.    Smita S. Aher, Saroj P. Gajare, Ravindra B. Saudagar. Linagliptin: A Review on Therapeutic Role in Diabetes Mellitus. Research Journal of Pharmacy and Technology 2017; 10(9):3233-3236.
3.    Kanase KG, Shinde NV et al.. Diabetes Macroangiopathy- A Review. Research Journal of Pharmacology and Pharmacodynamics.2009;2(1).
4.    Kanase KG, Shinde NV et al., Diabetes Macroangiopathy- A Review. Research Journal of Pharmacology and Pharmacodynamics.2009;2(1).
5.    Kamal A. et al. diabetes mellitus in rats. Journal of Brain research. 2006; 276-280.
6.    Michale J, Fowler MD Synaptic transmission changes in the pyramidal cells of the hippocampus in streptozotocin-induced. Microvascular and Macrovascular Complication of Diabetes. Clinical Diabetes 2010; 9(3): 116-122.
7.    Amar Gangadhar Zalte, Saudagar R.B. et al. Validated UV- Spectroscopic estimation of Linagliptin Concentration in Bulk and Dosage form. Research Journal of Pharmacy and Technology.2016;5(9):490-492.
8.    Murugan S, Upendra Janardhan CH, Niranjan Babu M. RP-HPLC Method for Simultaneous Estimation of Albendazole and Niclosamide in Oral Suspension for Veterinary Use. Research J. Pharm. and Tech.2016;9 (1):27-32.
9.    Saroj Gajare, Amar Zalte, Ravindra B. Saudagar.  Simultaneous Spectrophotometric Determination of Linagliptin and Metformin Hydrochloride in Combined Tablet Dosage Form. Asian Journal of Pharmaceutical Analysis 2017;7(2).
10.    Samir Derouiche, Khaoula Zeghib, Safa Gherbi, Yahia Khelef. Protective effects of Aristolochia longa and Aquilaria malaccensis against lead-induced oxidative stress in rat cerebrum. Asian J. Res. Pharm. Sci. 2019; 9(1):57-63.
11.    Report of a WHO consultation: Definition, diagnosis and classification of diabetes mellitus and its complications. World Health Organization department of non-communicable disease surveillance, Geneva; 1999: 7
12.    Tesfaye S, Selvarajah D. Advance in the Epidemiology, Pathogenesis and Management of Diabetic Peripheral Neuropathy. Diabetes Metab. Res. Rev. 2012; 28: 8-14.
13.    Tesfaye S, Selvarajah D. Advance in the Epidemiology, Pathogenesis and Management of Diabetic Peripheral Neuropathy. Diabetes Metab. Res. Rev. 2012; 28: 8-14.
14.    Kulkarni Sk.Handbook of experimental pharmacology.Vallabh Prakashan, 4th edition. 2016; 99-102.
15.    Sandireddy R, Yerra VG, Areti A, Komirishetty P and Kumar A. Neuroinflammation and Oxidative Stress in Diabetic Neuropathy: Futuristic Strategies Based on These Targets. International Journal of Endicrinology 2014; 1-10.
16.    Vogel HG, Drug Discovery and Evaluation; Pharmacological Assays. Springer Germany; IInd edition; 2002; 951.
17.    Mittal R, Kumar A, Singh DP, Bishnoi M, Nag TC. Ameliorating potential of rutin in combination with nimesulide in STZ model of diabetic neuropathy: targeting NrF2/HO-1/NF-ΚB and COX signalling pathway. Inflammopharmacol. 2017.
18.    Vinik AI, Mehrabyan A. Diabetic neuropathies. Medical Clinics of North America. 2004; 88(4): 947-999.
19.    M. Vaishali. Antioxidants in Health and Diseases. Research Journal of Pharmacy and Technology 2014; 7(4):489-493.
20.    U.S MahadevaRao, Khamsah Suryati Mohd et al. Screening of Phytochemicals and Comparative Antioxidant activity of Leaf and Fruit of MalaysianMengkudu Using Aqueous and Organic Solvent Extracts. Research Journal of Pharmacy and Technology 2013;6 (9):1064-1072.
21.    U.S MahadevaRao, Khamsah Suryati Mohd et al. Screening of Phytochemicals and Comparative Antioxidant activity of Leaf and Fruit of MalaysianMengkudu Using Aqueous and Organic Solvent Extracts. Research Journal of Pharmacy and Technology 2013;6 (9):1064-1072.
22.    Shaikh AS, Somani RS. Animal model and biomarkers of neuropathy in diabetic rodents. Indian J Pharmacol 2010; 42(3): 129-134.
23.    Vinik AI, Mehrabyan A. Diabetic neuropathies. Medical Clinics of North America. 2004; 88(4): 947-999.
24.    Shaikh AS, Somani RS. Animal model and biomarkers of neuropathy in diabetic rodents. Indian J Pharmacol 2010; 42(3): 129-134.
25.    Vogel HG, Drug Discovery and Evaluation; Pharmacological Assays. Springer Germany; IInd edition; 2002; 951.
26.    Rangarajan Narasimhan, Sathiyamoorthy M. Phytochemical Screening and Antioxidant Studies in the Pulp Extracts of Cucurbita maxima. Asian Journal of Pharmaceutical Research2016; 6(1): 1-10.
27.    Rangarajan Narasimhan, Sathiyamoorthy M. Phytochemical Screening and Antioxidant Studies in the Pulp Extracts of Cucurbita maxima. Asian Journal of Pharmaceutical Research. 2016; 6(1): 1-10.
28.    Mahadeva Rao, Khamsah Suryati Mohd, et al.    Screening of Phytochemicals and Comparative Antioxidant activity of Leaf and Fruit of Malaysian Mengkudu Using Aqueous and Organic Solvent Extracts. Research Journal of Pharmacy and Technology. 2013; 6(9).

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available