Author(s): Shravan Kumar Paswan, Sajal Srivastava, Chandana Venkateswara Rao

Email(s): ssrivastava2@lko.amity.edu

DOI: 10.5958/0974-360X.2020.00437.0   

Address: Shravan Kumar Paswan1,2, Sajal Srivastava2, Chandana Venkateswara Rao1
1Pharmacology Division, CSIR-National Botanical Research Institute, Lucknow-226001, Uttar Pradesh, India.
2Amity Institute of Pharmacy, Amity University, Uttar Pradesh, Lucknow Campus, Gomati Nagar, Lucknow- 226010, Uttar Pradesh, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 5,     Year - 2020


ABSTRACT:
The Aim of the present study to assess the Amaranthus spinonous in case of Incision wound model Anti-inflammatory and Analgesic activity (Hot plate, Tail flick method). The whole plant extraction was performed using 65% ethanol followed by phytochemical screening to determine secondary metabolites present in the plant. Wound healing activity of A. spinosus was determined using wound incision mode on rats. Animals were treated with two concentrations (5% and 10% w/w ointment) of A. spinosus and soframycin was used as standard and the study of analgesic and anti-inflammatory activity distributed in six different concentrations and observed different time period with the help of Plethysmometer at 0hr, 1hr, 2hr, 3hr, 4hr and 5hr after the administration of carrageenan to each group and Eddy Hot plate and Tail flick apparatus, the observation made at 0, 30, 60 and 90 min after oral administration of the samples. The evaluated the effect of 65% ethonolic extract of Amaranthus spinosus on incision wound, anti-inflammatory and analgesic activity. It has been observed that the ethonolic extract shown the maximum percentage protection toward inflammation comparison to other extract. The plant ASE prevents and reduces the development of inflammationin animals in carrageenan, hot plate and tail flick method. It can be concluded from the study that Amaranthus spinosus possess significant wound healing property and potential sources to treat inflammation.


Cite this article:
Shravan Kumar Paswan, Sajal Srivastava, Chandana Venkateswara Rao. Incision Wound healing, Anti-inflammatory and Analgesic activity of Amaranthus spinosus in Wistar rats. Research J. Pharm. and Tech 2020; 13(5): 2439-2444. doi: 10.5958/0974-360X.2020.00437.0

Cite(Electronic):
Shravan Kumar Paswan, Sajal Srivastava, Chandana Venkateswara Rao. Incision Wound healing, Anti-inflammatory and Analgesic activity of Amaranthus spinosus in Wistar rats. Research J. Pharm. and Tech 2020; 13(5): 2439-2444. doi: 10.5958/0974-360X.2020.00437.0   Available on: https://rjptonline.org/AbstractView.aspx?PID=2020-13-5-67


REFERENCES:
1.    Ayello, EA, Ads. Skin Wound Care. 2005; 18: 98-109.
2.    Rubin E, Fabrex JL. Repair, regeneration and fibrosis. In: Pathology, 2nd ed. J.B. Lippincott, Philadelphia, 1996; 7-15.
3.    Mantle D, Gok MA, Lennard TWJ. Adverse Drug Reactions and Toxicological Reviews. 2001; 20: 89-103.
4.    Martin P, Leibovich SJ. Inflammatory cells during wound repair: the good, the bad and the ugly. Trends Cell Biol. 2005;15: 599-607.
5.    Chan K. Some aspects of toxic contaminants in herbal medicines. Chemosphere. 2003; 52:1361–1371.
6.    Nagappa AN, Binu C. Wound healing activity of the aqueous extract of Thespesiapopulnea fruit. Fitotherapia. 2001; 72: 503-506.
7.    Kumar K, Singh KK, Asthana AK, Nath V. Ethno therapeutics of Bryophyte Plagiochasmaappendiculatum among the Gaddi Tribes of Kangra Valley, Hima-chal Pradesh, India. Pharm Biol. 2000; 38: 353–356.
8.    Martin P. Wound healing aiming for perfect skin regeneration. Science 1997; 276: 75–81.
9.    Ilhem R, Anouar BS, Najla H. Oxidative damage and hepatotoxicity associated with deltamethrin in rats: The protective effects of Amaranthus spinosus seed extract Biomedicine & Pharmacotherapy. 2016; 84: 853–860.
10.    Hilou OG. Nacoulma TR. Guiguemde. In vivo antimalarial activities of extracts from Amaranthus spinosus L. and Boerhaaviaerecta L. in mice, J. Ethnopharmacol. 2006; 103: 236–240.
11.    OECD. Acute dermal toxicity. In: OECD Draft Guidelines for Testing of Chemicals, No. 434, fixed procedures. Organization for Economic Cooperation and Development. Paris, France; 2004.
12.    Udupa AL, Rao SG, Kulkarni DR. Hound healing profile of septilin. Indian J Physiol Pharmacol. 1989; 33: 39-42.
13.    Govindarajan R, Vijayakumar M, Rao CV, Shirwaikar A, Mehrotra S, Puspangadan P. Healing potential of Anogeissuslatifolia for dermal wound in rats. Acta Pharm. 2004; 54: 331-338.
14.    Perez Gutierrez RM, Vargas SR. Evaluation of the wound healing properties of Acalyphalangiana in diabetic rats. Fitoterapia. 2006; 77: 286-289.
15.    Gbedema SY, Emelia K, Francis A, Kofi A, Eric W. Wound healing properties and kill kinetics of Clerodendronsplendens G. Don, A Ghanaian wound healing plant. Pharmacog. Res. 2010; 2: 63-68.
16.    Borgi W, Ghedira K, Chouchane N. Antiinflammatory and analgesic activities of Zizyphus lotus root barks. Fitoterapia. 2007; 78: 16-19.
17.    Vogel HG, Vogel WH. Drug Discovery and Evaluation. Verlag Berlin Heidelberg, New York, Springer, 1997; 390-417.
18.    Akindele AJ, Adeyemi OO. Anti inflammatoryactivity of the aqueous leaf extract of Byrsocarpuscoccineus. Fitoterapia. 2007; 78: 25-25.
19.    Dimo T, Fotio AL, Nguelefack TB, Asongalem EA, Kamtchouing P. Antiinflammatory activity of leaf extracts of Kalanchoecrenata Andr. Indian J Pharmacol. 2006; 38: 115-119.
20.    Raquibul Hasan SM, Hossain MM, Akter R, Jamila M, Mazumder MEH, Alam MA, Faruque A, Rana S, Rahman S. Analgesic Activity of the Different Fractions of the Aerial Parts of Commelinabenghalensis Linn. International Journal of Pharmacology. 2010; 6: 63-67.
21.    Rajnarayana K, Reddy MS, Chaluvadi MR, Krishna DR. Biflavonoids classification, pharmacological, biochemical effects and therapeutic potential. Indian Journal of Pharmacology. 2001; 33: 2-16.
22.    Rao MR, Rao YM, Rao AV, Prabhkar MC, Rao CS, Muralidhar N. Antinociceptive and antiinflammatory activity of a flavonoid isolated from Caralluma attenuate. Journal of Ethnopharmacology. 1998; 62: 63-66.
23.    Vanu MR, Palanivelu S, Panchanatham S. Immunomodulatory and anti-inflammatory effects of Semecarpusanacardium Linn. Nut milk extract in experimental inflammatory conditions. Biological and Pharmaceutical Bulletin. 2006; 29: 693-700.
24.    Assefa E, Alemayhu I, Endale M, Mammo F. Iridoid glycosides from the root of Acanthus sennii. Journal of Pharmacy and Pharmacognosy Research. 2016; 4: 231–237.
25.    Getie M, Gebre-Mariam T, Rietz R, et al. Evaluation of the anti-microbial and anti-inflammatory activities of the medicinal plants Dodonaeaviscosa, Rumexnervosus and Rumexabyssinicus. Fitoterapia. 2003; 74: 139-143.
26.    Arun M, Satish S, Anima P. Evaluation of wound healing, antioxidant and antimicrobial efficacy of Jasminumauriculatum Vahl. Leaves. Avicenna Journal of Phytomedicine. 2016; 6: 295.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

0.38
2018CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available